Three Gram-positive, catalase-negative, motile, rod-shaped bacteria were isolated from fermented stinky tofu brine. These strains, designated YIT 11306, YIT 11317 and YIT 11318, were discriminated from five isolates on the basis of randomly amplified polymorphic DNA profiles. They produced -lactic acid as the main end product from glucose without gas formation, synthesized dextran from sucrose and hydrolysed aesculin. Ammonia was not produced from arginine. Comparative 16S rRNA gene sequence analysis demonstrated that the novel isolates were members of the genus . Based on levels of 16S rRNA gene sequence similarity, the three novel strains were related most closely to the type strains of (97.2 %) and (96.8 %). However, levels of DNA–DNA relatedness between the novel isolates and the type strains of and were less than 10 %. The phenotypic and genotypic data demonstrate that the three strains represent a single novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIT 11306 (=JCM 15044=BCRC 17811=DSM 19910).


Article metrics loading...

Loading full text...

Full text loading...



  1. Akopyanz, N., Bukanov, N. O., Westblom, T. U., Kresovich, S. & Berg, D. E.(1992). DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD fingerprinting. Nucleic Acids Res 20, 5137–5142.[CrossRef] [Google Scholar]
  2. Barrow, G. I. & Feltham, R. K. (editors)(1993).Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. London: Cambridge University Press.
  3. Carr, J. G. & Davies, P. A.(1970). Homofermentative lactobacilli of ciders including Lactobacillus mali nov. spec. J Appl Bacteriol 33, 768–774.[CrossRef] [Google Scholar]
  4. Cavalli-Sforza, L. L. & Edwards, A. W. F.(1967). Phylogenetic analysis models and estimation procedures. Am J Hum Genet 19, 233–257. [Google Scholar]
  5. Endo, A. & Okada, S.(2005).Lactobacillus satsumensis sp. nov., isolated from mashes of shochu, a traditional Japanese distilled spirit made from fermented rice and other starchy materials. Int J Syst Evol Microbiol 55, 83–85.[CrossRef] [Google Scholar]
  6. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  7. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  8. Felsenstein, J.(2007).phylip (phylogeny interface package), version 3.67. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  9. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  10. Kaneuchi, C., Seki, M. & Komagata, K.(1988). Taxonomic study of Lactobacillus mali Carr and Davis 1970 and related strains: validation of Lactobacillus mali Carr and Davis 1970 over Lactobacillus yamanashiensis Nonomura 1983. Int J Syst Bacteriol 38, 269–272.[CrossRef] [Google Scholar]
  11. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  12. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  13. Miyake, T., Watanabe, K., Watanabe, T. & Oyaizu, H.(1998). Phylogenetic analysis of the genus Bifidobacterium and related genera based on 16S rDNA sequences. Microbiol Immunol 42, 661–667.[CrossRef] [Google Scholar]
  14. Nonomura, H.(1983).Lactobacillus yamanashiensis subsp. yamanashiensis and Lactobacillus yamanashiensis subsp. mali sp. and subsp. nov., nom. rev. Int J Syst Bacteriol 33, 406–407.[CrossRef] [Google Scholar]
  15. Rodas, A. M., Chenoll, E., Macián, M. C., Ferrer, S., Pardo, I. & Aznar, R.(2006).Lactobacillus vini sp. nov., a wine lactic acid bacterium homofermentative for pentoses. Int J Syst Evol Microbiol 56, 513–517.[CrossRef] [Google Scholar]
  16. Saitou, N. & Nei, M.(1987). The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  17. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  18. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  19. Zhu, H., Qu, F. & Zhu, L. H.(1993). Isolation of genomic DNAs from plants, fungi, and bacteria using benzyl chloride. Nucleic Acids Res 21, 5279–5280.[CrossRef] [Google Scholar]

Data & Media loading...


Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences showing the relationship of strains YIT 11306 , YIT 11317 and YIT 11318 to closely related species. [PDF](17 KB)


Maximum-likelihood phylogenetic tree based on 16S rRNA gene sequences showing the relationship of strains YIT 11306 , YIT 11317 and YIT 11318 to closely related species. [PDF](17 KB)

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error