1887

Abstract

Three Gram-positive, catalase-negative, motile, rod-shaped bacteria were isolated from fermented stinky tofu brine. These strains, designated YIT 11306, YIT 11317 and YIT 11318, were discriminated from five isolates on the basis of randomly amplified polymorphic DNA profiles. They produced -lactic acid as the main end product from glucose without gas formation, synthesized dextran from sucrose and hydrolysed aesculin. Ammonia was not produced from arginine. Comparative 16S rRNA gene sequence analysis demonstrated that the novel isolates were members of the genus . Based on levels of 16S rRNA gene sequence similarity, the three novel strains were related most closely to the type strains of (97.2 %) and (96.8 %). However, levels of DNA–DNA relatedness between the novel isolates and the type strains of and were less than 10 %. The phenotypic and genotypic data demonstrate that the three strains represent a single novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIT 11306 (=JCM 15044=BCRC 17811=DSM 19910).

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2008-11-01
2019-10-22
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Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences showing the relationship of strains YIT 11306 , YIT 11317 and YIT 11318 to closely related species. [PDF](17 KB)

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Maximum-likelihood phylogenetic tree based on 16S rRNA gene sequences showing the relationship of strains YIT 11306 , YIT 11317 and YIT 11318 to closely related species. [PDF](17 KB)

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