1887

Abstract

Two strains of marine, heterotrophic, alkaliphilic bacteria, designated A3F-7 and ssthio04PA2-7c, were isolated from hard coral and marine sand, respectively, collected in the Republic of Palau. A phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates were related to members of the genus . However, the sequence similarities between the isolates and the type strains of the species were less than 93 %. The G+C contents of the isolates were around 57 mol%, the major respiratory quinone was Q-8 and the predominant cellular fatty acids were 16 : 17, 16 : 0, 18 : 17 and 14 : 0. On the basis of the phylogenetic data, phenotypic characteristics and DNA–DNA hybridization results, strains A3F-7 and ssthio04PA2-7c represent a novel species of a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is A3F-7 (=MBIC06429 =DSM 19956).

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2009-02-01
2020-01-23
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References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef]
    [Google Scholar]
  2. Barrow, G. I. & Feltham, R. K. A. ( 1993; ). Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press.
  3. Benediktsdóttir, E., Verdonck, L., Spróer, C., Helgason, S. & Swings, J. ( 2000; ). Characterization of Vibrio viscosus and Vibrio wodanis isolated at different geographical locations: a proposal for reclassification of Vibrio viscosus as Moritella viscosa comb. nov. Int J Syst Evol Microbiol 50, 479–488.[CrossRef]
    [Google Scholar]
  4. Bowman, J. P. ( 2005; ). Genus XIII. Shewanella MacDonell and Colwell 1986, 355VP. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part B, pp. 480–491. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  5. Bowman, J. P. & McMeekin, T. A. ( 2005; ). Order X. Alteromonadales ord. nov. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part B, p. 443. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  6. Bowman, J. P., McCammon, S. A., Nichols, D. S., Skerratt, J. H., Rea, S. M., Nichols, P. D. & McMeekin, T. A. ( 1997; ). Shewanella gelidimarina sp. nov. and Shewanella frigidimarina sp. nov., novel Antarctic species with the ability to produce eicosapentaenoic acid (20 : 5ω3) and grow anaerobically by dissimilatory Fe(III) reduction. Int J Syst Bacteriol 47, 1040–1047.[CrossRef]
    [Google Scholar]
  7. Bozal, N., Montes, M. J., Tudela, E., Jiménez, F. & Guinea, J. ( 2002; ). Shewanella frigidimarina and Shewanella livingstonensis sp. nov. isolated from Antarctic coastal areas. Int J Syst Evol Microbiol 52, 195–205.
    [Google Scholar]
  8. Brettar, I., Christen, R. & Höfle, M. G. ( 2002; ). Shewanella denitrificans sp. nov., a vigorously denitrifying bacterium isolated from the oxic–anoxic interface of the Gotland Deep in the central Baltic Sea. Int J Syst Evol Microbiol 52, 2211–2217.[CrossRef]
    [Google Scholar]
  9. Campbell, S., Harada, R. M. & Li, Q. X. ( 2007; ). Ferrimonas senticii sp. nov., a novel gammaproteobacterium isolated from the mucus of a puffer fish caught in Kaneohe Bay, Hawai'i. Int J Syst Evol Microbiol 57, 2670–2673.[CrossRef]
    [Google Scholar]
  10. DSMZ ( 1993; ). Catalogue of Strains, 5th edn. Braunschweig: Gesellschaft für Biotechnologische Forschung.
  11. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  12. Gao, H., Obraztova, A., Stewart, N., Popa, R., Fredrickson, J. K., Tiedje, J. M., Nealson, K. H. & Zhou, J. ( 2006; ). Shewanella loihica sp. nov., isolated from iron-rich microbial mats in the Pacific Ocean. Int J Syst Evol Microbiol 56, 1911–1916.[CrossRef]
    [Google Scholar]
  13. Hirota, K., Nodasaka, Y., Orikasa, Y., Okuyama, H. & Yumoto, I. ( 2005; ). Shewanella pneumatophori sp. nov., an eicosapentaenoic acid-producing marine bacterium isolated from the intestines of Pacific mackerel (Pneumatophorus japonicus). Int J Syst Evol Microbiol 55, 2355–2359.[CrossRef]
    [Google Scholar]
  14. Hosoya, S. & Yokota, A. ( 2007; ). Loktanella atrilutea sp. nov., isolated from seawater in Japan. Int J Syst Evol Microbiol 57, 1966–1969.[CrossRef]
    [Google Scholar]
  15. Hosoya, S., Arunpairojana, V., Suwannachart, C., Kanjana-Opas, A. & Yokota, A. ( 2006; ). Aureispira marina gen. nov., sp. nov., a gliding, arachidonic acid-containing bacterium isolated from the southern coastline of Thailand. Int J Syst Evol Microbiol 56, 2931–2935.[CrossRef]
    [Google Scholar]
  16. Ivanova, E. P., Sawabe, T., Gorshkova, N. M., Svetashev, V. I., Mikhailov, V. V., Nicolau, D. V. & Christen, R. ( 2001; ). Shewanella japonica sp. nov. Int J Syst Evol Microbiol 51, 1027–1033.[CrossRef]
    [Google Scholar]
  17. Ivanova, E. P., Nedashkovskaya, O. I., Zhukova, N. V., Nicolau, D. V., Christen, R. & Mikhailov, V. V. ( 2003; ). Shewanella waksmanii sp. nov., isolated from a sipuncula (Phascolosoma japonicum). Int J Syst Evol Microbiol 53, 1471–1477.[CrossRef]
    [Google Scholar]
  18. Ivanova, E. P., Gorshkova, N. M., Bowman, J. P., Lysenko, A. M., Zhukova, N. V., Sergeev, A. F., Mikhailov, V. V. & Nicolau, D. V. ( 2004a; ). Shewanella pacifica sp. nov., a polyunsaturated fatty acid-producing bacterium isolated from sea water. Int J Syst Evol Microbiol 54, 1083–1087.[CrossRef]
    [Google Scholar]
  19. Ivanova, E. P., Flavier, A. & Christen, R. ( 2004b; ). Phylogenetic relationships among marine Alteromonas-like proteobacteria: emended description of the family Alteromonadaceae and proposal of Pseudoalteromonadaceae fam. nov., Colwelliaceae fam. nov., Shewanellaceae fam. nov., Moritellaceae fam. nov., Ferrimonadaceae fam. nov., Idiomarinaceae fam. nov. and Psychromonadaceae fam. nov. Int J Syst Evol Microbiol 54, 1773–1788.[CrossRef]
    [Google Scholar]
  20. Katsuta, A., Adachi, K., Matsuda, S., Shizuri, Y. & Kasai, H. ( 2005; ). Ferrimonas marina sp. nov. Int J Syst Evol Microbiol 55, 1851–1855.[CrossRef]
    [Google Scholar]
  21. Khan, S. T. & Harayama, S. ( 2007; ). Paraferrimonas sedimenticola gen. nov., sp. nov., a marine bacterium of the family Ferrimonadaceae. Int J Syst Evol Microbiol 57, 1493–1498.[CrossRef]
    [Google Scholar]
  22. Kim, H. J., Park, S., Lee, J. M., Park, S., Jung, W., Kang, J.-S., Joo, H. M., Seo, K.-W. & Kang, S.-H. ( 2008; ). Moritella dasanensis sp. nov., a psychrophilic bacterium isolated from the Arctic ocean. Int J Syst Evol Microbiol 58, 817–820.[CrossRef]
    [Google Scholar]
  23. Lee, O. O., Lau, S. C. K., Tsoi, M. M. Y., Li, X., Plakhotnikova, I., Dobretsov, S., Wu, M. C. S., Wong, P.-K., Weinbauer, M. & Qian, P.-Y. ( 2006; ). Shewanella irciniae sp. nov., a novel member of the family Shewanellaceae, isolated from the marine sponge Ircinia dendroides in the Bay of Villefranche, Mediterranean Sea. Int J Syst Evol Microbiol 56, 2871–2877.[CrossRef]
    [Google Scholar]
  24. Leonardo, M. R., Moser, D. P., Barbieri, E., Brantner, C. A., MacGregor, B. J., Paster, B. J., Stackebrandt, E. & Nealson, K. H. ( 1999; ). Shewanella pealeana sp. nov., a member of the microbial community associated with the accessory nidamental gland of the squid Loligo pealei. Int J Syst Bacteriol 49, 1341–1351.[CrossRef]
    [Google Scholar]
  25. Lunder, T., Sørum, H., Holstad, G., Steigerwalt, A. G., Mowinckel, P. & Brenner, D. J. ( 2000; ). Phenotypic and genotypic characterization of Vibrio viscosus sp. nov. and Vibrio wodanis sp. nov. isolated from Atlantic salmon (Salmo salar) with ‘winter ulcer’. Int J Syst Evol Microbiol 50, 427–450.[CrossRef]
    [Google Scholar]
  26. Miyazaki, M., Nogi, Y., Usami, R. & Horikoshi, K. ( 2006; ). Shewanella surugensis sp. nov., Shewanella kaireitica and Shewanella abyssi sp. nov., isolated from deep-sea sediments of Suruga Bay, Japan. Int J Syst Evol Microbiol 56, 1607–1613.[CrossRef]
    [Google Scholar]
  27. Nakagawa, T., Iino, T., Suzuki, K. & Harayama, S. ( 2006; ). Ferrimonas futtsuensis sp. nov. and Ferrimonas kyonanensis sp. nov., selenate-reducing bacteria belonging to the Gammaproteobacteria isolated from Tokyo Bay. Int J Syst Evol Microbiol 56, 2639–2645.[CrossRef]
    [Google Scholar]
  28. Nogi, Y. & Kato, C. ( 1999; ). Taxonomic studies of extremely barophilic bacteria isolated from the Mariana Trench and description of Moritella yayanosii sp. nov., a new barophilic bacterial isolate. Extremophiles 3, 71–77.[CrossRef]
    [Google Scholar]
  29. Nogi, Y., Kato, C. & Horikoshi, K. ( 1998a; ). Taxonomic studies of deep-sea barophilic Shewanella strains and description of Shewanella violacea sp. nov. Arch Microbiol 170, 331–338.[CrossRef]
    [Google Scholar]
  30. Nogi, Y., Kato, C. & Horikoshi, K. ( 1998b; ). Moritella japonica sp. nov., a novel barophilic bacterium isolated from a Japan Trench sediment. J Gen Appl Microbiol 44, 289–295.[CrossRef]
    [Google Scholar]
  31. Rosselló-Mora, R. A., Ludwig, W., Kämpfer, P., Amann, R. & Schleifer, K.-H. ( 1995; ). Ferrimonas balearica gen. nov., spec. nov., a new marine facultative Fe(III)-reducing bacterium. Syst Appl Microbiol 18, 196–202.[CrossRef]
    [Google Scholar]
  32. Saito, H. & Miura, K. ( 1963; ). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72, 619–629.[CrossRef]
    [Google Scholar]
  33. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  34. Satomi, M., Oikawa, H. & Yano, Y. ( 2003; ). Shewanella marinintestina sp. nov., Shewanella schlegeliana sp. nov. and Shewanella sairae sp. nov., novel eicosapentaenoic-acid-producing marine bacteria isolated from sea-animal intestines. Int J Syst Evol Microbiol 53, 491–499.[CrossRef]
    [Google Scholar]
  35. Satomi, M., Fonnesbech Vogel, B., Gram, L. & Venkateswaran, K. ( 2006; ). Shewanella hafniensis sp. nov. and Shewanella morhuae sp. nov., isolated from marine fish of the Baltic Sea. Int J Syst Evol Microbiol 56, 243–249.[CrossRef]
    [Google Scholar]
  36. Satomi, M., Fonnesbech Vogel, B., Venkateswaran, K. & Gram, L. ( 2007; ). Description of Shewanella glacialipiscicola sp. nov. and Shewanella algidipiscicola sp. nov., isolated from marine fish of the Danish Baltic Sea, and proposal that Shewanella affinis is a later heterotypic synonym of Shewanella colwelliana. Int J Syst Evol Microbiol 57, 347–352.[CrossRef]
    [Google Scholar]
  37. Skerratt, J. H., Bowman, J. P. & Nichols, P. D. ( 2002; ). Shewanella olleyana sp. nov., a marine species isolated from a temperate estuary which produced high levels of polyunsaturated fatty acids. Int J Syst Evol Microbiol 52, 2101–2106.[CrossRef]
    [Google Scholar]
  38. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  39. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  40. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  41. Toffin, L., Bidault, A., Pignet, P., Tindall, B. J., Slobodkin, A., Kato, C. & Prieur, D. ( 2004; ). Shewanella profunda sp. nov., isolated from deep marine sediment of the Nankai Trough. Int J Syst Evol Microbiol 54, 1943–1949.[CrossRef]
    [Google Scholar]
  42. Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K. & Colwell, R. R. ( 1998; ). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS Microbiol Lett 165, 373–378.[CrossRef]
    [Google Scholar]
  43. Venkateswaran, K., Moser, D. P., Dollhopf, M. E., Lies, D. P., Saffarini, D. A., MacGregor, B. J., Ringelberg, D. B., White, D. C., Nishijima, M. & other authors ( 1999; ). Polyphasic taxonomy of the genus Shewanella and description of Shewanella oneidensis sp. nov. Int J Syst Bacteriol 49, 705–724.[CrossRef]
    [Google Scholar]
  44. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  45. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J. ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703.
    [Google Scholar]
  46. Xiao, X., Wang, P., Zeng, X., Bartlett, D. H. & Wang, F. ( 2007; ). Shewanella psychrophila sp. nov. and Shewanella piezotolerans sp. nov., isolated from west Pacific deep-sea sediment. Int J Syst Evol Microbiol 57, 60–65.[CrossRef]
    [Google Scholar]
  47. Xu, Y., Nogi, Y., Kato, C., Liang, Z., Rüger, H.-J., De Kegel, D. & Glansdorff, N. ( 2003; ). Moritella profunda sp. nov. and Moritella abyssi sp. nov., two psychropiezophilic organisms isolated from deep Atlantic sediments. Int J Syst Evol Microbiol 53, 533–538.[CrossRef]
    [Google Scholar]
  48. Xu, M., Guo, J., Cen, Y., Zhong, X., Cao, W. & Sun, G. ( 2005; ). Shewanella decolorationis sp. nov., a dye-decolorizing bacterium isolated from activated sludge of a waste-water treatment plant. Int J Syst Evol Microbiol 55, 363–368.[CrossRef]
    [Google Scholar]
  49. Yang, S.-H., Kwon, K. K., Lee, H.-S. & Kim, S.-J. ( 2006; ). Shewanella spongiae sp. nov., isolated from a marine sponge. Int J Syst Evol Microbiol 56, 2879–2882.[CrossRef]
    [Google Scholar]
  50. Yang, S.-H., Lee, J.-H., Ryu, J.-S., Kato, C. & Kim, S.-J. ( 2007; ). Shewanella donghaensis sp. nov., a psychrophilic, piezosensitive bacterium producing high levels of polyunsaturated fatty acid, isolated from deep-sea sediments. Int J Syst Evol Microbiol 57, 208–212.[CrossRef]
    [Google Scholar]
  51. Yoon, J.-H., Kang, K. H., Oh, T.-K. & Park, Y.-H. ( 2004a; ). Shewanella gaetbuli sp. nov., a slight halophile isolated from a tidal flat in Korea. Int J Syst Evol Microbiol 54, 487–491.[CrossRef]
    [Google Scholar]
  52. Yoon, J.-H., Yeo, S.-H., Kim, I.-G. & Oh, T.-K. ( 2004b; ). Shewanella marisflavi sp. nov. and Shewanella aquimarina sp. nov., slightly halophilic organisms isolated from sea water of the Yellow Sea in Korea. Int J Syst Evol Microbiol 54, 2347–2352.[CrossRef]
    [Google Scholar]
  53. Zhao, J.-S., Manno, D., Beaulieu, C., Paquet, L. & Hawari, J. ( 2005; ). Shewanella sediminis sp. nov., a Na+-requiring and hexahydro-1,3,5-trinitro-1,3,5-triazine-degrading bacterium from marine sediment. Int J Syst Evol Microbiol 55, 1511–1520.[CrossRef]
    [Google Scholar]
  54. Zhao, J.-S., Manno, D., Leggiadro, C., O'Neil, D. & Hawari, J. ( 2006; ). Shewanella halifaxensis sp. nov., a novel obligately respiratory denitrifying psychrophile. Int J Syst Evol Microbiol 56, 205–212.[CrossRef]
    [Google Scholar]
  55. Zhao, J.-S., Manno, D., Thiboutot, S., Ampleman, G. & Hawari, J. ( 2007; ). Shewanella canadensis sp. nov. and Shewanella atlantica sp. nov., manganese dioxide- and hexahydro-1,3,5-trinitro-1,3,5-triazine-reducing, psychrophilic marine bacteria. Int J Syst Evol Microbiol 57, 2155–2162.[CrossRef]
    [Google Scholar]
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