1887

Abstract

A Gram-positive, moderately halophilic bacterium, designated strain EN8d, was isolated from sediment from Lake Erliannor in the Inner Mongolia Autonomous Region, China. Cells were facultatively anaerobic, rod-shaped and motile and did not display endospore formation. Isolate EN8d grew in a complex medium supplemented with 0–20 % (w/v) marine salts (optimally at 5–7.5 %, w/v). Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that strain EN8d was a member of the family , belonging to a cluster with (96.3 % gene sequence similarity) and (95.0–96.0 %), albeit emerging as an independent lineage from members of these two genera. Strain EN8d contained cell-wall peptidoglycan based on -diaminopimelic acid and possessed MK-7 as the major respiratory isoprenoid quinone. The major fatty acids were anteiso-C and anteiso-C. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid. The DNA G+C content was 47.5 mol%. Strain EN8d could be clearly differentiated from its phylogenetic neighbours on the basis of several phenotypic, genotypic and chemotaxonomic features. Therefore strain EN8d is considered to represent a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is EN8d (=CCM 7364 =CECT 7148 =CGMCC 1.6199 =DSM 18088).

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2008-08-01
2020-01-21
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