1887

Abstract

Six acetic acid bacterial isolates, obtained during a study of the microbial diversity of spontaneous fermentations of Ghanaian cocoa beans, were subjected to a polyphasic taxonomic study. (GTG)-PCR fingerprinting grouped the isolates together, but they could not be identified using this method. Phylogenetic analysis based on 16S rRNA gene sequences allocated the isolates to the genus and revealed , and to be nearest neighbours. DNA–DNA hybridizations demonstrated that the isolates belonged to a single novel genospecies that could be differentiated from its phylogenetically nearest neighbours by the following phenotypic characteristics: no production of 2-keto--gluconic acid from -glucose; growth on methanol and -xylose, but not on maltose, as sole carbon sources; no growth on yeast extract with 30 % -glucose; and weak growth at 37 °C. The DNA G+C contents of four selected strains were 56.8–58.0 mol%. The results obtained prove that the isolates should be classified as representatives of a novel species, for which the name sp. nov. is proposed. The type strain is strain 985 (=R-36330 =LMG 24244 =DSM 19596).

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2008-09-01
2019-10-14
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Maximum-parsimony phylogenetic tree based on nearly complete 16S rRNA gene sequences. [PDF](18 KB)

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Additional differential characteristics of sp. nov. and phylogenetically related species. [PDF](17 KB)

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