1887

Abstract

A filamentous actinomycete, designated strain D65, was isolated from a root of a wild tree, D. Don (Nepalese Alder), collected in Xishuangbanna, China. It produced the bioactive agents daidzein and -acetyltyramine and had morphological and chemical properties characteristic of streptomycetes. Pink to brownish red diffusible pigments were produced on several ISP media. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain D65 formed a distinct phyletic line that was most closely, albeit loosely, associated with YIM 001, NRRL B-5416, NBRC 12874, NBRC 15428 and NBRC 13473. A number of phenotypic properties allowed differentiation of the strain from related species. Therefore strain D65 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is D65 (=CGMCC 4.3510=NRRL B-24611).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65769-0
2009-02-01
2020-01-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/2/254.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65769-0&mimeType=html&fmt=ahah

References

  1. Bérdy, J. ( 2005; ). Bioactive microbial metabolites. J Antibiot 58, 1–26.[CrossRef]
    [Google Scholar]
  2. Chun, J. & Goodfellow, M. ( 1995; ). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45, 240–245.[CrossRef]
    [Google Scholar]
  3. Collins, M. D. ( 1985; ). Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  4. Coombs, J. T. & Franco, C. M. M. ( 2003; ). Isolation and identification of actinobacteria from surface-sterilized wheat roots. Appl Environ Microbiol 69, 5603–5608.[CrossRef]
    [Google Scholar]
  5. Dietera, A., Hamm, A., Fiedler, H.-P., Goodfellow, M., Müller, W. E. G., Brun, R., Beil, W. & Bringmann, G. ( 2003; ). Pyrocoll, an antibiotic, antiparasitic and antitumor compound produced by a novel alkalophilic Streptomyces strain. J Antibiot 56, 639–646.[CrossRef]
    [Google Scholar]
  6. Evtushenko, L. I., Akimov, V. N., Dobritsa, S. V. & Taptykova, S. D. ( 1989; ). A new species of actinomycete, Amycolata alni. Int J Syst Bacteriol 39, 72–77.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  8. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  9. Gu, Q., Luo, H., Zheng, W., Liu, Z. & Huang, Y. ( 2006; ). Pseudonocardia oroxyli sp. nov., a novel actinomycete isolated from surface-sterilized Oroxylum indicum root. Int J Syst Evol Microbiol 56, 2193–2197.[CrossRef]
    [Google Scholar]
  10. Gurney, K. A. & Mantle, P. G. ( 1993; ). Biosynthesis of 1-N-methylalbonoursin by an endophytic Streptomyces sp. isolated from perennial ryegrass. J Nat Prod 56, 1194–1198.[CrossRef]
    [Google Scholar]
  11. Hasegawa, T., Takizawa, M. & Tanida, S. ( 1983; ). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–332.[CrossRef]
    [Google Scholar]
  12. Kämpfer, P., Kroppenstedt, R. M. & Dott, W. ( 1991; ). A numerical classification of the genera Streptomyces and Streptoverticillium using miniaturized physiological tests. J Gen Microbiol 137, 1831–1891.[CrossRef]
    [Google Scholar]
  13. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  14. Kudo, T., Matsushima, K., Itoh, T., Sasaki, J. & Suzuki, K. I. ( 1998; ). Description of four new species of the genus Kineosporia: Kineosporia succinea sp. nov., Kineosporia rhizophila sp. nov., Kineosporia mikuniensis sp. nov. and Kineosporia rhamnosa sp. nov., isolated from plant samples, and amended description of the genus Kineosporia. Int J Syst Bacteriol 48, 1245–1255.[CrossRef]
    [Google Scholar]
  15. Labeda, D. P., Lechevalier, M. P. & Testa, R. T. ( 1997; ). Streptomyces stramineus sp. nov., a new species of the verticillate streptomycetes. Int J Syst Bacteriol 47, 747–753.[CrossRef]
    [Google Scholar]
  16. Lechevalier, H. A. & Lechevalier, M. P. ( 1980; ). The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy, Special Publication no. 6, pp. 277–284. Edited by A. Dietz & D. W. Thayer. Arlington, VA: Society for Industrial Microbiology.
  17. Lechevalier, M. P., De Bièvre, C. & Lechevalier, H. A. ( 1977; ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef]
    [Google Scholar]
  18. Manfio, G. P., Zakrzewska-Czerwinska, J., Atalan, E. & Goodfellow, M. ( 1995; ). Towards minimal standards for the description of Streptomyces species. Biotekhnologia 7–8, 242–253.
    [Google Scholar]
  19. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  20. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  21. Saitou, N. & Nei, M. ( 1987; ). The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  22. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  23. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  24. Williams, S. T., Goodfellow, M., Alderson, G., Wellington, E. M. H., Sneath, P. H. A. & Sackin, M. J. ( 1983; ). Numerical classification of Streptomyces and related genera. J Gen Microbiol 129, 1743–1813.
    [Google Scholar]
  25. Williams, S. T., Goodfellow, M. & Alderson, G. ( 1989; ). Genus Streptomyces Waksman and Henrici 1943, 339AL. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2452–2492. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65769-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65769-0
Loading

Data & Media loading...

Supplements

vol. , part 2, pp. 254 - 258

Scanning electron micrographs showing spore chains and spore-surface ornamentation of cells of strain D65 ( sp. nov.) grown on ISP 2 agar. [PDF](55 KB)



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error