A novel moderately thermophilic bacterium, designated strain BT 13, was isolated from a geothermal water source in Dolni Bogrov, near Sofia, Bulgaria. The isolate was spore-forming, Gram-positive, facultatively anaerobic, alkalitolerant and heterotrophic, and was able to ferment a wide variety of carbon sources including -glucose, sucrose, -arabinose, -rhamnose, starch, sorbitol and glycogen. Strain BT 13 grew optimally at pH 8.0 and 65 °C. Intracellular amylolytic activity was registered with glucose as the main product of starch hydrolysis. Phylogenetic analysis based on the 16S rRNA gene revealed that the strain belonged to the genus , the closest relatives being and . The DNA G+C content was 44.1 mol%. The fatty acid profile with a content of iso-branched fatty acids of around 80 % of the total fatty acids is similar to that of recognized species. On the basis of genotypic differentiation and significant differences in phenotypic characteristics, it was concluded that strain BT 13 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BT 13 (=DSM 17956=NBIMCC 8427).


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  1. Belduz, A. O., Dulger, S. & Demirbag, Z.(2003).Anoxybacillus gonensis sp. nov., a moderately thermophilic, xylose-utilizing, endospore-forming bacterium. Int J Syst Evol Microbiol 53, 1315–1320.[CrossRef] [Google Scholar]
  2. Boone, D. R., Liu, Y., Zhao, Z. J., Balkwill, D. L., Drake, G. R., Stevens, T. O. & Aldrich, H. C.(1995).Bacillus infernus sp. nov., an Fe(III)- and Mn(IV)-reducing anaerobe from the deep terrestrial subsurface. Int J Syst Bacteriol 45, 441–448.[CrossRef] [Google Scholar]
  3. Derekova, A., Sjøholm, C., Mandeva, R. & Kambourova, M.(2007).Anoxybacillus rupiensis sp. nov., a novel thermophilic bacterium isolated from Rupi basin (Bulgaria). Extremophiles 11, 577–583.[CrossRef] [Google Scholar]
  4. Dulger, S., Demirbag, Z. & Belduz, A. O.(2004).Anoxybacillus ayderensis sp. nov. and Anoxybacillus kestanbolensis sp. nov. Int J Syst Evol Microbiol 54, 1499–1503.[CrossRef] [Google Scholar]
  5. Heinen, W., Lauwers, A. M. & Mulders, J. W. M.(1982).Bacillus flavothermus, a newly isolated facultative thermophile. Antonie van Leeuwenhoek 48, 265–272.[CrossRef] [Google Scholar]
  6. Johansen, J. E., Nielsen, P. & Sjøholm, C.(1999). Description of Cellulophaga baltica gen. nov., sp. nov. and Cellulophaga fucicola gen. nov., sp. nov. and reclassification of [Cytophaga] lytica to Cellulophaga lytica gen. nov., comb. nov. Int J Syst Bacteriol 49, 1231–1240.[CrossRef] [Google Scholar]
  7. Kevbrin, V. V., Zengler, K., Lysenko, A. M. & Wiegel, J.(2005).Anoxybacillus kamchatkensis sp. nov., a novel thermophilic facultative aerobic bacterium with a broad pH optimum from the Geyser valley, Kamchatka. Extremophiles 9, 391–398.[CrossRef] [Google Scholar]
  8. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  9. Miller, G. L.(1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31, 426–428.[CrossRef] [Google Scholar]
  10. Pantschev, C., Klenz, G. & Hafner, B.(1981). Vergleichende Charakterisierung von Alfa-Amylasepraparaten. Lebensmittelindustrie 28, 71–74. (. in German [Google Scholar]
  11. Pikuta, E., Lysenko, A., Chuvilskaya, N., Mendrock, U., Hippe, H., Suzina, N., Nikitin, D., Osipov, G. & Laurinavichius, K.(2000).Anoxybacillus pushchinensis gen. nov., sp. nov., a novel anaerobic, alkaliphilic, moderately thermophilic bacterium from manure, and description of Anoxybacillus flavithermus comb. nov. Int J Syst Evol Microbiol 50, 2109–2117.[CrossRef] [Google Scholar]
  12. Pikuta, E., Cleland, D. & Tang, J.(2003). Aerobic growth of Anoxybacillus pushchinoensis K1T: emended descriptions of A. pushchinoensis and the genus Anoxybacillus. Int J Syst Evol Microbiol 53, 1561–1562.[CrossRef] [Google Scholar]
  13. Poli, A., Esposito, E., Lama, L., Orlando, P., Nicolaus, G., de Appolonia, F., Gambacorta, A. & Nicolaus, B.(2006).Anoxybacillus amylolyticus sp. nov., a thermophilic amylase producing bacterium from Mount Rittmann (Antarctica). Syst Appl Microbiol 29, 300–307.[CrossRef] [Google Scholar]
  14. Smibert, R. M. & Krieg, N. R.(1981). General characterization. In Manual of Methods for General Microbiology, pp. 409–443. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Washington, DC: American Society for Microbiology.
  15. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  16. Strunck, O. & Ludwig, W.(1995). ARB – a software environment for sequence data, Department of Microbiology, University of Munich, Munich, Germany. Available at http://www.arb-home.de

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