A Gram-positive, alkaliphilic bacterium, designated strain BA288, was isolated from sandy soil. Cells were facultatively anaerobic, endospore-forming rods that were motile by means of peritrichous flagella. The strain grew at 15–40 °C and pH 7.0–11.0 (optimally at 30 °C and pH 9.0–9.5) and at salinities of 0–4 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain BA288 belonged to the genus and that D-1,5a, LMG 22167, DSM 6307 and SW-211 were the closest neighbours (96.2, 96.0, 96.0 and 95.9 % sequence similarity, respectively). The genomic DNA G+C content was 37.9 mol% and the predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C, iso-C, C and iso-C. The peptidoglycan type was A1 (-diaminopimelic acid). Therefore, on the basis of phylogenetic, phenotypic and chemotaxonomic properties, strain BA288 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BA288 (=KCTC 3947 =DSM 16976).


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vol. , part 11, pp. 2629 - 2634

Maximum-likelihood (Fig. S1) and maximum-parsimony (Fig. S2) phylogenetic trees, based on 16S rRNA gene sequences, showing the relationships between strain BA 288 and related taxa. Numbers at branching nodes are bootstrap percentages (based on 1000 replications); only values ≥50% are indicated. Bars, 0.1 substitutions per nucleotide position. [PDF](29 KB)


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