1887

Abstract

A novel marine actinomycete, designated strain SSW1-48, was isolated from a dried seaweed sample collected from a beach in Jeju, Republic of Korea. Cells of the organism were non-motile, non-endospore-forming, Gram-positive and rod-shaped. Colonies were circular, smooth, translucent and yellow-coloured. The diagnostic diamino acid in the peptidoglycan was 2,4-diaminobutyric acid and the murein was of the acetyl type. Mycolic acids were absent. The predominant menaquinones were MK-10 and MK-9. The major fatty acids were anteiso-C and iso-C. The G+C content of the DNA was 73.0 mol%. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that the isolate formed a distinct branch between and in the family . The values for 16S rRNA gene sequence similarity between strain SSW1-48 and type strains of recognized species were in the range 95.5–97.5 % and those between this bacterium and other representatives of the family were in the range 93.7–96.4 %. On the basis of the combined data from the phenotypic and phylogenetic analyses, strain SSW1-48 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SSW1-48 (=KCTC 19198=JCM 14256).

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2008-10-01
2020-01-20
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References

  1. Bora, N., Vancanneyt, M., Gelsomino, R., Swings, J., Brennan, N., Cogan, T. M., Larpin, S., Desmasures, N., Lechner, F. E. & other authors ( 2007; ). Agrococcus casei sp. nov., isolated from the surfaces of smear-ripened cheeses. Int J Syst Evol Microbiol 57, 92–97.[CrossRef]
    [Google Scholar]
  2. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  3. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  4. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  5. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef]
    [Google Scholar]
  6. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  7. Hopwood, D. A., Bibb, M. J., Chater, K. F., Kieser, T., Bruton, C. J., Kieser, H. M., Lydiate, D. J., Smith, C. P., Ward, J. M. & other authors ( 1985; ). Genetic Manipulation of Streptomyces: a Laboratory Manual. Norwich: John Innes Foundation.
  8. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  9. Kroppenstedt, R. M. ( 1985; ). Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series, vol. 20), pp. 173–199. Edited by M. Goodfellow & D. E. Minnikin. New York: Academic Press.
  10. Lee, S. D. ( 2006; ). Blastococcus jejuensis sp. nov., an actinomycete from beach sediment, and emended description of the genus Blastococcus Ahrens and Moll 1970. Int J Syst Evol Microbiol 56, 2391–2396.[CrossRef]
    [Google Scholar]
  11. Lee, S. D. ( 2007; ). Labedella gwakjiensis gen. nov., sp. nov., a novel actinomycete of the family Microbacteriaceae. Int J Syst Evol Microbiol 57, 2498–2502.[CrossRef]
    [Google Scholar]
  12. Lee, S. D. & Jeong, H. S. ( 2006; ). Actinomadura hallensis sp. nov., a novel actinomycete isolated from Mt. Halla in Korea. Int J Syst Evol Microbiol 56, 259–264.[CrossRef]
    [Google Scholar]
  13. Lee, S. D., Kang, S.-O. & Hah, Y. C. ( 2000; ). Hongia gen. nov., a new genus of the order Actinomycetales. Int J Syst Evol Microbiol 50, 191–199.[CrossRef]
    [Google Scholar]
  14. MacFaddin, J. F. ( 1980; ). Biochemical Tests for Identification of Medical Bacteria, 2nd edn. Baltimore: Williams & Wilkins.
  15. Mayilraj, S., Suresh, K., Schumann, P., Kroppenstedt, R. M. & Saini, H. S. ( 2006; ). Agrococcus lahaulensis sp. nov., isolated from a cold desert of the Indian Himalayas. Int J Syst Evol Microbiol 56, 1807–1810.[CrossRef]
    [Google Scholar]
  16. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  17. Minnikin, D. E., Patel, P. V., Alshamaony, L. & Goodfellow, M. ( 1977; ). Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27, 104–117.[CrossRef]
    [Google Scholar]
  18. Minnikin, D. E., Hutchinson, I. G., Caldicott, A. B. & Goodfellow, M. ( 1980; ). Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188, 221–233.[CrossRef]
    [Google Scholar]
  19. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  20. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  21. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  22. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  23. Uchida, K. & Aida, K. ( 1984; ). An improved method for the glycolate test for simple identification of acyl type of bacterial cell walls. J Gen Appl Microbiol 30, 131–134.[CrossRef]
    [Google Scholar]
  24. Wieser, M., Schumann, P., Martin, K., Altenburger, P., Burghardt, J., Lubitz, W. & Busse, H. J. ( 1999; ). Agrococcus citreus sp. nov., isolated from a medieval wall painting of the chapel of Castle Herberstein (Austria). Int J Syst Bacteriol 49, 1165–1170.[CrossRef]
    [Google Scholar]
  25. Zlamala, C., Schumann, P., Kämpfer, P., Rosselló-Mora, R., Lubitz, W. & Busse, H. J. ( 2002; ). Agrococcus baldri sp. nov., isolated from the air in the ‘Virgilkapelle’ in Vienna. Int J Syst Evol Microbiol 52, 1211–1216.[CrossRef]
    [Google Scholar]
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Transmission electron micrograph of cells of strain SSW1-48 ( sp. nov.). [PDF](319 KB)



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