1887

Abstract

A Gram-negative, facultatively anaerobic, motile, slightly curved rod-shaped bacterial strain was isolated from black sand collected from Soesoggak, Jeju island, Korea. The strain, designated J74, was able to grow in the presence of 1–7.5 % NaCl, at temperatures of 4–45 °C and at pH 5–10. Strain J74 was oxidase- and catalase-positive, arginine dihydrolase-negative and sensitive to the vibriostatic agent O/129. Strain J74 was characterized based on physiology, morphology, biochemical features and 16S rRNA gene sequence analysis. The isolate required sodium ions for growth and utilized a wide range of compounds as sole sources of carbon and energy. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain J74 belongs to the class . It was found to be associated with the genus and was phylogenetically related most closely to the type strain of (98.7 % 16S rRNA gene sequence similarity). However, DNA–DNA hybridization experiments between strain J74 and KCTC 12827 revealed a level of relatedness of 37.7 %. Thus, phenotypic and phylogenetic data suggested that J74 should be placed in the genus as representing a novel species, for which the name sp. nov. is proposed. The type strain is J74 (=KCTC 22122 =JCM 14949).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65726-0
2008-08-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/8/1903.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65726-0&mimeType=html&fmt=ahah

References

  1. Austin, B. ( 1988; ). Marine Microbiology. Cambridge: Cambridge University Press.
  2. Chun, J., Lee, J. H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  3. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  4. Gonzalez, J. M., Mayer, F., Moran, M. A., Hodson, R. E. & Whitman, W. B. ( 1997; ). Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. Int J Syst Bacteriol 47, 369–376.[CrossRef]
    [Google Scholar]
  5. Gordon, R. E., Haynes, W. C. & Pang, C. H.-N. ( 1973; ). The Genus Bacillus, US Department of Agriculture Handbook no. 427. Washington, DC: US Department of Agriculture.
  6. Hjeltnes, B. & Roberts, R. J. ( 1993; ). Vibriosis. In Bacterial Diseases of Fish, pp. 109–121. Edited by V. Inglis, R. J. Roberts & N. R. Bromage. Oxford: Blackwell Scientific.
  7. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  8. Kushmaro, A., Banin, E., Loya, Y., Stackebrandt, E. & Rosenberg, E. ( 2001; ). Vibrio shiloi sp. nov., the causative agent of bleaching of the coral Oculina patagonica. Int J Syst Evol Microbiol 51, 1383–1388.
    [Google Scholar]
  9. Lambert, C., Nicolas, J. L., Cilia, V. & Corre, S. ( 1998; ). Vibrio pectenicida sp. nov., a pathogen of scallop (Pecten maximus) larvae. Int J Syst Bacteriol 48, 481–487.[CrossRef]
    [Google Scholar]
  10. Lightner, D. V. ( 1993; ). Diseases of cultured penaeid shrimp. In CRC Handbook of Mariculture, 2nd edn, vol. 1, pp. 393–486. Edited by J. P. McVey. Boca Raton, FL: CRC Press.
  11. Pacini, F. ( 1854; ). Osservazione microscopiche e deduzioni patologiche sul cholera asiatico. Gaz Med Ital Toscano Firenze 6, 405–412 (in Italian).
    [Google Scholar]
  12. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  13. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  14. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  15. Stackebrandt, E., Liesack, W. & Goebel, B. M. ( 1993; ). Bacterial diversity in a soil sample from a subtropical Australian environment as determined by 16S rDNA analysis. FASEB J 7, 232–236.
    [Google Scholar]
  16. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  17. Thompson, F. L., Li, Y., Gomez-Gil, B., Thompson, C. C., Hoste, B., Vandemeulebroecke, K., Rupp, G. S., Pereira, A., De Bem, M. M. & other authors ( 2003; ). Vibrio neptunius sp. nov., Vibrio brasiliensis sp. nov. and Vibrio xuii sp. nov., isolated from the marine aquaculture environment (bivalves, fish, rotifers and shrimps). Int J Syst Evol Microbiol 53, 245–252.[CrossRef]
    [Google Scholar]
  18. Thompson, F. L., Iida, T. & Swings, J. ( 2004; ). Biodiversity of vibrios. Microbiol Mol Biol Rev 68, 403–431.[CrossRef]
    [Google Scholar]
  19. Yoon, J. H., Lee, S. T. & Park, Y. H. ( 1998; ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65726-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65726-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error