Skip to content
1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 58, Issue 9

sp. nov., isolated from the Pindari glacier of the Indian Himalayas, and emended description of the genus Free

Abstract

Strain PON10 is a yellow-pigmented, Gram-positive, aerobic, motile, rod-shaped bacterium isolated from the Pindari glacier of the Indian Himalayas. The cell-wall peptidoglycan contained -diaminobutyric acid as the diamino acid. The predominant fatty acids were anteiso-C, anteiso-C and iso-C and the major isoprenoid quinones were MK-10 and MK-11. Based on the above characteristics, strain PON10 was assigned to the genus . sequence similarity results indicated that and were the nearest relatives, with 16S rRNA gene sequence similarity of 97.0 and 96.8 % to the respective type strains. A difference of 3 % in the 16S rRNA gene sequence indicated that PON10 represents a novel species of the genus , and therefore DNA–DNA hybridization was not done. In addition, PON10 showed a number of differences from and with respect to phenotypic and chemotaxonomic characteristics. Thus, based on the differences it exhibited from and strain PON10 was identified as representing a novel species named sp. nov. The type strain is PON10 (=LMG 24222 =MTCC9128). An emended description of the genus is also presented.

  • Published Online:
Keyword(s): DAB, dl-2,4-diaminobutyric acid
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65715-0
2008-09-01
2025-04-30
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/9/2229.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65715-0&mimeType=html&fmt=ahah

References

  1. Ageta, Y. & Kadota, T.(1992). Predictions of changes of glacier mass balance in the Nepal Himalaya and Tibetan Plateau: a case study of air temperature increase for three glaciers. Ann Glaciol 16, 89–94. [Google Scholar]
  2. Chaturvedi, P. & Shivaji, S.(2006).Exiguobacterium indicum sp. nov., a psychrophilic bacterium from the Hamta glacier of the Himalayan mountain ranges of India. Int J Syst Evol Microbiol 56, 2765–2770.[CrossRef] [Google Scholar]
  3. Chaturvedi, P., Reddy, G. S. N. & Shivaji, S.(2005).Dyadobacter hamtensis sp. nov, from Hamta glacier, located in the Himalayas, India. Int J Syst Evol Microbiol 55, 2113–2117.[CrossRef] [Google Scholar]
  4. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef] [Google Scholar]
  5. Davis, M. J., Gillaspie, A. G., Jr, Vidaver, A. K. & Harris, R. W.(1984).Clavibacter: a new genus containing some phytopathogenic coryneform bacteria, including Clavibacter xyli subsp. xyli sp. nov., subsp. nov. and Clavibacter xyli subsp cynodontis subsp. nov., pathogens that cause ratoon stunting disease of sugarcane and burmudagrass stunting disease. Int J Syst Bacteriol 34, 107–117.[CrossRef] [Google Scholar]
  6. Dyurgerov, M. B. & Meier, M. F.(1997). Mass balance of mountain and subpolar glaciers: a new global assessment for 1961–1990. Arct Alp Res 29, 379–391.[CrossRef] [Google Scholar]
  7. Evtushenko, L. I., Dorofeeva, L. V., Subbotin, S. A., Cole, J. R. & Tiedje, J. M.(2000).Leifsonia poae gen. nov., sp. nov., isolated from nematode galls on Poa annua, and reclassification of ‘Corynebacterium aquaticum’ Leifson 1962 as Leifsonia aquatica (ex Leifson 1962) gen. nov., nom. rev., comb. nov. and Clavibacter xyli Davis et al. 1984 with two subspecies as Leifsonia xyli (Davis et al. 1984) gen. nov., comb. nov. Int J Syst Evol Microbiol 50, 371–380.[CrossRef] [Google Scholar]
  8. Evtushenko, L. I., Dorofeeva, L. V., Dobrovolskaya, T. G., Streshinskaya, G. M., Subbotin, S. A. & Tiedje, J. M.(2001).Agreia bicolorata gen. nov., sp. nov., to accommodate actinobacteria isolated from narrow reed grass infected by the nematode Heteroanguina graminophila. Int J Syst Evol Microbiol 51, 2073–2079.[CrossRef] [Google Scholar]
  9. Fushimi, H., Ikegami, K., Higuchi, K. & Shankar, K.(1985). Nepal case study: catastrophic floods. In Techniques for Prediction of Runoff from Glacierized Areas (publication 149), pp. 125–130. Edited by G. J. Young. Wallingford, UK: International Association of Hydrological Sciences.
  10. Ghosh, A., Bhardwaj, M., Satyanarayana, T., Khurana, M., Mayilraj, S. & Jain, R. K.(2007).Bacillus lehensis sp. nov., an alkalitolerant bacterium isolated from soil. Int J Syst Evol Microbiol 57, 238–242.[CrossRef] [Google Scholar]
  11. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  12. Komagata, K. & Suzuki, K.(1987). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19, 161–206. [Google Scholar]
  13. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  14. Lányí, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  15. Leifson, E.(1962). The bacterial flora of distilled and stored water. III. New species of the genera Corynebacterium, Flavobacterium, Spirillum and Pseudomonas. Int Bull Bacteriol Nomencl Taxon 12, 161–170. [Google Scholar]
  16. Männistö, M. K., Schumann, P., Rainey, F. A., Kämpfer, P., Tsitko, I., Tiirola, M. A. & Salkinoja-Salonen, M. S.(2000).Subtercola boreus gen. nov., sp. nov. and Subtercola frigoramans sp. nov., two new psychrophilic actinobacteria isolated from boreal groundwater. Int J Syst Evol Microbiol 50, 1731–1739. [Google Scholar]
  17. Mayilraj, S., Prasad, G. S., Suresh, K., Saini, H. S., Shivaji, S. & Chakrabarti, T.(2005).Planococcus stackebrandtii sp. nov., isolated from a cold desert of the Himalayas, India. Int J Syst Evol Microbiol 55, 91–94.[CrossRef] [Google Scholar]
  18. Mayilraj, S., Krishnamurthi, S., Saha, P. & Saini, H. S.(2006a).Rhodococcus kroppenstedtii sp. nov., a novel actinobacterium isolated from a cold desert of the Himalayas, India. Int J Syst Evol Microbiol 56, 979–982.[CrossRef] [Google Scholar]
  19. Mayilraj, S., Saha, P., Suresh, K. & Saini, H. S.(2006b).Ornithinimicrobium kibberense sp. nov., isolated from the Indian Himalayas. Int J Syst Evol Microbiol 56, 1657–1661.[CrossRef] [Google Scholar]
  20. Mayilraj, S., Suresh, K., Kroppenstedt, R. M. & Saini, H. S.(2006c).Dietzia kunjamensis sp. nov., isolated from the Indian Himalayas. Int J Syst Evol Microbiol 56, 1667–1671.[CrossRef] [Google Scholar]
  21. Mayilraj, S., Suresh, K., Schumann, P., Kroppenstedt, R. M. & Saini, H. S.(2006d).Agrococcus lahaulensis sp. nov., isolated from a cold desert of the Indian Himalayas. Int J Syst Evol Microbiol 56, 1807–1810.[CrossRef] [Google Scholar]
  22. Mayilraj, S., Kroppenstedt, R. M., Suresh, K. & Saini, H. S.(2006e).Kocuria himachalensis sp. nov., an actinobacterium isolated from the Indian Himalayas. Int J Syst Evol Microbiol 56, 1971–1975.[CrossRef] [Google Scholar]
  23. Qiu, F., Huang, Y., Sun, L., Zhang, X., Liu, Z. & Song, W.(2007).Leifsonia ginsengi sp. nov., isolated from ginseng root. Int J Syst Evol Microbiol 57, 405–408.[CrossRef] [Google Scholar]
  24. Reddy, G. S. N., Aggarwal, R. K., Matsumoto, G. I. & Shivaji, S.(2000).Arthrobacter flavus sp. nov., a psychrophilic bacterium isolated from a pond in McMurdo Dry Valley, Antarctica. Int J Syst Evol Microbiol 50, 1553–1561.[CrossRef] [Google Scholar]
  25. Reddy, G. S. N., Prakash, J. S. S., Srinivas, R., Matsumoto, G. I. & Shivaji, S.(2003).Leifsonia rubra sp. nov. and Leifsonia aurea sp. nov., psychrophiles from a pond in Antarctica. Int J Syst Evol Microbiol 53, 977–984.[CrossRef] [Google Scholar]
  26. Reddy, G. S. N., Matsumoto, G. I., Schumann, P., Stackebrandt, E. & Shivaji, S.(2004). Psychrophilic pseudomonads from Antarctica: Pseudomonas antarctica sp. nov., Pseudomonas meridiana sp. nov. and Pseudomonas proteolytica sp. nov. Int J Syst Evol Microbiol 54, 713–719.[CrossRef] [Google Scholar]
  27. Reddy, G. S. N., Nagy, M. & Garcia-Pichel, F.(2006).Belnapia moabensis gen. nov., sp. nov., an alphaproteobacterium from biological soil crusts in the Colorado Plateau, USA. Int J Syst Evol Microbiol 56, 51–58.[CrossRef] [Google Scholar]
  28. Shivaji, S., Chaturvedi, P., Reddy, G. S. N. & Suresh, K.(2005).Pedobacter himalayensis sp. nov., from the Hamta glacier located in the Himalayan mountain ranges of India. Int J Syst Evol Microbiol 55, 1083–1088.[CrossRef] [Google Scholar]
  29. Singla, A. K., Mayilraj, S., Kudo, T., Krishnamurthi, S., Prasad, G. S. & Vohra, R. M.(2005).Actinoalloteichus spitiensis sp. nov., a novel actinobacterium isolated from a cold desert of the Indian Himalayas. Int J Syst Evol Microbiol 55, 2561–2564.[CrossRef] [Google Scholar]
  30. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  31. Suresh, K., Reddy, G. S. N., Sengupta, S. & Shivaji, S.(2004).Deinococcus indicus sp. nov., an arsenic-resistant bacterium from an aquifer in West Bengal, India. Int J Syst Evol Microbiol 54, 457–461.[CrossRef] [Google Scholar]
  32. Suzuki, K. T., Suzuki, M., Sasaki, J., Park, Y. H. & Komagata, K.(1999).Leifsonia gen. nov., a genus for 2,4-diaminobutyric acid-containing actinomycetes to accommodate “Corynebacterium aquaticum” Leifson 1962 and Clavibacter xyli subsp. cynodontis Davis et al. 1984. J Gen Appl Microbiol 45, 253–262.[CrossRef] [Google Scholar]
  33. Tamaoka, J.(1986). Analysis of bacterial menaquinone mixtures by reverse-phase high-performance liquid chromatography. Methods Enzymol 123, 251–256. [Google Scholar]
  34. Tamaoka, J., Katayama-Fujimura, Y. & Kuraishi, H.(1983). Analysis of bacterial menaquinone mixture by high performance liquid chromatography. J Appl Bacteriol 54, 31–36.[CrossRef] [Google Scholar]
  35. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  36. Yamada, T.(1991).Preliminary Report on Glacier Lake Outburst Flood in the Nepal Himalayas, Report 4/1/291191/1/l, Seq. No. 387. Kathmandu, Nepal: Water and Energy secretariat, HMG.
  37. Yamada, T., Shiraiwa, T., Iida, H., Kadota, T., Watanabe, T., Rana, B., Ageta, Y. & Fushimi, H.(1992). Fluctuations of the glaciers from the 1970s to 1989 in the Khumbu, Shorong and Langtang regions, Nepal Himalayas. Bull Glaciol Res 10, 11–19. [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.65715-0
Loading
Leifsonia pindariensis sp. nov., isolated from the Pindari glacier of the Indian Himalayas, and emended description of the genus Leifsonia
Int J Syst Evol Microbiol 58, 2229 (2008); https://doi.org/10.1099/ijs.0.65715-0
/content/journal/ijsem/10.1099/ijs.0.65715-0
/content/journal/ijsem/10.1099/ijs.0.65715-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error