Five Gram-negative, rod-shaped, moderately halophilic and denitrifying strains, designated XK1, XK2, XK3, XK4 and XK5, were isolated from a saline and alkaline soil in Korla, north-western China. These isolates could grow anaerobically using either nitrate or nitrite as terminal electron acceptors and produced gas from nitrate vigorously. A comparison and phylogenetic analysis of 16S rRNA gene sequences placed these isolates in the genus within the family . The isolates shared the highest 16S rRNA gene sequence similarities with Al12(95.6 %), YKJ-16 (95.5 %) and SL014B-85 (95.2 %) (values determined by 3.1; direct comparison results with GenBank were even lower, not ≥94 %). Sequence similarities with other recognized species were below 95.0 %, far below the 97.0 % threshold generally accepted for the delineation of separate species. BOX-PCR fingerprinting and DNA–DNA hybridization showed high similarities among the five strains which indicated they were members of the same species. The major fatty acids were C 8, C and C 7. The DNA G+C content was 65.3 mol%. All the results of the phenotypic and genetic analyses supported the hypothesis that the five new strains represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is XK1 (=CGMCC 1.6981=DSM 19633).


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Transmission electron micrograph of cells of strain XK1 . [ PDF] 70 KB


Neighbour-joining phylogenetic relationships between the five strains of sp. nov. and other species of the family . [ PDF] 40 KB


BOX-PCR fingerprint patterns of the five novel isolates. [ PDF] 67 KB


Differential phenotypic characteristics among strains XK1 , XK2, XK3, XK4 and XK5. [ PDF] 39 KB


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