1887

Abstract

Members of the saltwater genus , formerly known as the marine , are obligate predatory bacteria that prey selectively on other Gram-negative bacteria. Previous phylogenetic analysis based on the 16S rRNA genes of saltwater isolates from environmental samples revealed 11 distinct phylogenetic clusters based on ≥96.5 % gene sequence similarity. In other micro-organisms, the gene coding for the -subunit of RNA polymerase () has been shown to be more discriminating than 16S rRNA genes. In this study, sequences from isolates were analysed to determine whether the results would be consistent with those based on 16S rRNA gene sequences. A 1242 bp region of the gene from 74 saltwater strains and two freshwater isolates, Uki2 and A3.12, was amplified by PCR and analysed. The sequences were aligned and phylogenetic trees were constructed using a neighbour-joining algorithm. The resulting tree showed that the sequences produced smaller subdivisions of isolates, but were nevertheless consistent with the clusters determined using 16S rRNA gene sequences. Thus, the highly conserved 16S rRNA gene sequences provided good phylogenetic information and the gene sequences permitted greater differentiation in order to further subdivide phylogenetically distinct groups within the genus . Also, on the basis of the extensive diversity and large distance between the saltwater members of the genus and the freshwater/soil , a reclassification of as gen. nov., comb. nov. is proposed. A new family, fam. nov., is also described.

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2008-05-01
2019-09-15
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