An actinomycete strain 3-44-a(19) was isolated from sandy soil collected in Bangladesh. The strain formed irregular pseudosporangia directly from aggregated spore chains above the rudimentary aerial mycelium. The pseudosporangia developed singly. Each pseudosporangium contained many small, non-motile, spherical, smooth-surfaced spores in chains. Strain 3-44-a(19) contained - and 3-hydroxydiaminopimelic acid in the cell wall and MK-9(H) as the major menaquinone and arabinose, galactose, glucose, mannose, ribose and xylose were present in the whole-cell hydrolysate. The diagnostic phospholipid was phosphatidylethanolamine and iso-C (24.6 %), C 9 (15.5 %), C (10.6 %), C (9.4 %), iso-C (8.6 %) and anteiso-C (6.0 %) were detected as the major cellular fatty acids. The acyl type of the peptidoglycan was glycolyl and mycolic acids were not detected. The G+C content of the DNA was 73.6 mol%. The chemotaxonomic data indicated that the strain belonged to the family . Phylogenetic analysis based on 16S rRNA gene sequence data also suggested that strain 3-44-a(19) fell within the family . On the basis of phylogenetic analysis and characteristic patterns of 16S rRNA gene signature nucleotides as well as morphological and chemotaxonomic data, this strain should be classified as a member of a new genus and species, gen. nov., sp. nov., in the family . The type strain of is 3-44-a(19) (=JCM 14710 =MTCC9007).


Article metrics loading...

Loading full text...

Full text loading...



  1. Ara, I. & Kudo, T.(2006). Three novel species of the genus Catellatospora, Catellatospora chokoriensis sp. nov., Catellatospora coxensis sp. nov. and Catellatospora bangladeshensis sp. nov., and transfer of Catellatospora citrea subsp. methionotrophica Asano and Kawamoto 1988 to Catellatospora methionotrophica sp. nov., comb. nov. Int J Syst Evol Microbiol 56, 393–400.[CrossRef] [Google Scholar]
  2. Ara, I. & Kudo, T.(2007a).Luedemannella gen. nov., a new genus of the family Micromonosporaceae and description of Luedemannella helvata sp. nov. and Luedemannella flava sp. nov. J Gen Appl Microbiol 53, 39–51.[CrossRef] [Google Scholar]
  3. Ara, I. & Kudo, T.(2007b). Two new species of the genus Micromonospora: Micromonospora chokoriensis sp. nov. and Micromonospora coxensis sp. nov., isolated from sandy soil. J Gen Appl Microbiol 53, 29–37.[CrossRef] [Google Scholar]
  4. Ara, I. & Kudo, T.(2007c).Krasilnikovia gen. nov., a new member of the family Micromonosporaceae and description of Krasilnikovia cinnamonea sp. nov. Actinomycetologica 21, 1–10.[CrossRef] [Google Scholar]
  5. Ara, I. & Kudo, T.(2007d).Sphaerosporangium gen. nov., a new member of the family Streptosporangiaceae, with descriptions of three new species as Sphaerosporangium melleum sp. nov., Sphaerosporangium rubeum sp. nov. and Sphaerosporangium cinnabarinum sp. nov., and transfer of Streptosporangium viridialbum Nonomura and Ohara 1960 to Sphaerosporangium viridialbum comb. nov. Actinomycetologica 21, 11–21.[CrossRef] [Google Scholar]
  6. Asano, K. & Kawamoto, I.(1986).Catellatospora, a new genus of the Actinomycetales. Int J Syst Bacteriol 36, 512–517.[CrossRef] [Google Scholar]
  7. Becker, B., Lechevalier, M. P. & Lechevalier, H. A.(1965). Chemical composition of cell-wall preparations from strains of various form-genera of aerobic actinomycetes. Appl Microbiol 13, 236–243. [Google Scholar]
  8. Brosius, J., Palmer, J. L., Kennedy, J. P. & Noller, H. F.(1978). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75, 4801–4805.[CrossRef] [Google Scholar]
  9. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef] [Google Scholar]
  10. Collins, M. D., Faulkner, M. & Keddie, R. M.(1984). Menaquinone composition of some spore forming actinomycetes. Syst Appl Microbiol 5, 20–29.[CrossRef] [Google Scholar]
  11. Couch, J. N.(1950).Actinoplanes. A new genus of the Actinomycetales. J Elisha Mitchell Sci Soc 66, 87–92. [Google Scholar]
  12. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  13. Goodfellow, M.(1991). The family Streptosporangiaceae. In The Prokaryotes, 2nd edn, pp. 1115–1138. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  14. Goodfellow, M. L., Stanton, J., Simpson, K. E. & Minnikin, D. E.(1990). Numerical and chemical classification of Actinoplanes and some related actinomycetes. J Gen Microbiol 136, 19–36.[CrossRef] [Google Scholar]
  15. Hayakawa, M. & Nonomura, H.(1987). Humic acid-vitamin agar, a new medium for selective isolation of soil actinomycetes. J Ferment Technol 65, 501–509.[CrossRef] [Google Scholar]
  16. Horan, A. C. & Brodsky, B.(1986).Actinoplanes caeruleus sp. nov., a blue-pigmented species of the genus Actinoplanes. Int J Syst Bacteriol 36, 187–191.[CrossRef] [Google Scholar]
  17. Jacobson, E., Grauville, W. C. & Fogs, C. E.(1958).Color Harmony Manual, 4th edn. Chicago: Container Corporation of America.
  18. Kane, W. D.(1966). A new genus of the Actinoplanaceae, Pilimelia, with a description of two species, Pilimelia terevasa and Pilimelia anulata. J Elisha Mitchell Sci Soc 82, 220–230. [Google Scholar]
  19. Koch, C., Kroppenstedt, R. M., Rainey, F. A. & Stackebrandt, E.(1996). 16S ribosomal DNA analysis of the genera Micromonospora, Actinoplanes, Catellatospora, Catenuloplanes, Couchioplanes, Dactylosporangium, and Pilimelia and emendation of the family Micromonosporaceae. Int J Syst Bacteriol 46, 765–768.[CrossRef] [Google Scholar]
  20. Krasil'nikov, N. A.(1938).Ray Fungi and Related Organisms – Actinomycetales. Moscow: Izdatel'stvo Akademii Nauk SSSR (in Russian).
  21. Kroppenstedt, R. M.(1985). Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20), pp. 173–199. Edited by M. Goodfellow & D. E. Minnikin. New York: Academic Press.
  22. Kroppenstedt, R. & Goodfellow, M.(1991). The family Thermomonosporaceae. In The Prokaryotes, 2nd edn, pp. 1085–1114. Edited by A. Balows, H. G. Trüper, M, Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  23. Kudo, T., Nakajima, Y. & Suzuki, K.(1999).Catenuloplanes crispus (Petrolini et al. 1993) comb. nov.: incorporation of the genus Planopolyspora Petrolini 1993 into the genus Catenuloplanes Yokota et al. 1993 with an amended description of the genus Catenuloplanes. Int J Syst Bacteriol 49, 1853–1860.[CrossRef] [Google Scholar]
  24. Lechevalier, M. P. & Lechevalier, H. A.(1970). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef] [Google Scholar]
  25. Lechevalier, M. P., Stern, A. E. & Lechevalier, H. A.(1981). Phospholipids in the taxonomy of actinomycetes. Zentralbl Bakteriol Hyg Abt 1 (Suppl. 11), 111–116. [Google Scholar]
  26. Lee, S. D. & Hah, Y. C.(2002). Proposal to transfer Catellatospora ferrugineum and ‘Catellatospora ishikariense’ to Asanoa gen. nov. as Asanoa ferrugineum comb. nov. and Asanoa ishikariensis sp. nov., with the emended description of the genus Catellatospora. Int J Syst Evol Microbiol 52, 967–972.[CrossRef] [Google Scholar]
  27. Lee, S. D., Kang, S.-O. & Hah, Y. C.(2000).Catellatospora koreensis sp. nov., a novel actinomycete isolated from a gold-mine cave. Int J Syst Evol Microbiol 50, 1103–1111.[CrossRef] [Google Scholar]
  28. Maldonado, L. A., Fenical, W., Jensen, P. R., Kauffman, C. A., Mincer, T. J., Ward, A. C., Bull, A. T. & Goodfellow, M.(2005).Salinispora arenicola gen. nov., sp. nov. and Salinispora tropica sp. nov., obligate marine actinomycetes belonging to the family Micromonosporaceae. Int J Syst Evol Microbiol 55, 1759–1766.[CrossRef] [Google Scholar]
  29. Matsumoto, A., Takahashi, Y., Shinose, M., Seino, A., Iwai, Y. & Omura, S.(2003).Longispora albida gen. nov., sp. nov., a novel genus of the family Micromonosporaceae. Int J Syst Evol Microbiol 53, 1553–1559.[CrossRef] [Google Scholar]
  30. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  31. Nakajima, Y., Kitpreechavanich, V., Suzuki, K. & Kudo, T.(1999).Microbispora corallina sp. nov., a new species of the genus Microbispora isolated from Thai soil. Int J Syst Bacteriol 49, 1761–1767.[CrossRef] [Google Scholar]
  32. Ørskov, J.(1923).Investigations into the Morphology of the Ray Fungi. Copenhagen: Levin and Munksgaard.
  33. Raeder, U. & Broda, P.(1985). Rapid preparation of DNA from filamentous fungi. Lett Appl Microbiol 1, 17–20.[CrossRef] [Google Scholar]
  34. Rheims, H., Schumann, P., Rohde, M. & Stackebrandt, E.(1998).Verrucosispora gifhornensis gen. nov., sp. nov., a new member of the actinobacterial family Micromonosporaceae. Int J Syst Bacteriol 48, 1119–1127.[CrossRef] [Google Scholar]
  35. Saito, H. & Miura, K.(1963). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72, 619–629.[CrossRef] [Google Scholar]
  36. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  37. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  38. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  39. Stackebrandt, E. & Kroppenstedt, R. M.(1987). Union of the genera Actinoplanes Couch, Ampullariella Couch, and Amorphosporangium Couch in a redefined genus Actinoplanes. Syst Appl Microbiol 9, 110–114.[CrossRef] [Google Scholar]
  40. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L.(1997). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef] [Google Scholar]
  41. Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231. [Google Scholar]
  42. Tamaoka, J.(1994). Determination of DNA base composition. In Chemical Methods in Prokaryotic Systematics, pp. 463–470. Edited by M. Goodfellow & A. G. O'Donnell. Chichester: Wiley.
  43. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  44. Tamaoka, J., Katayama-Fujimura, Y. & Kuraishi, H.(1983). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54, 31–36.[CrossRef] [Google Scholar]
  45. Tamura, T. & Hatano, K.(2001). Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and Actinoplanes aurantiacus to Cryptosporangium minutisporangius comb. nov. and Cryptosporangium aurantiacum sp. nov. Int J Syst Evol Microbiol 51, 2119–2125.[CrossRef] [Google Scholar]
  46. Tamura, T., Nakagaito, Y., Nishii, T., Hasegawa, T., Stackebrandt, E. & Yokota, A.(1994). A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov. Int J Syst Bacteriol 44, 193–203.[CrossRef] [Google Scholar]
  47. Tamura, T., Hayakawa, M. & Hatano, K.(1997). A new genus of the order Actinomycetales, Spirilliplanes gen. nov., with description of Spirilliplanes yamanashiensis sp. nov. Int J Syst Bacteriol 47, 97–102.[CrossRef] [Google Scholar]
  48. Tamura, T., Hayakawa, M. & Hatano, K.(2001). A new genus of the order Actinomycetales, Virgisporangium gen. nov., with descriptions of Virgisporangium ochraceum sp. nov. and Virgisporangium aurantiacum sp. nov. Int J Syst Evol Microbiol 51, 1809–1816.[CrossRef] [Google Scholar]
  49. Tamura, T., Hatano, K. & Suzuki, K.(2006). A new genus of the family Micromonosporaceae, Polymorphospora gen. nov., with description of Polymorphospora rubra sp. nov. Int J Syst Evol Microbiol 56, 1959–1964.[CrossRef] [Google Scholar]
  50. Thawai, C., Tanasupawat, S., Itoh, T. & Kudo, T.(2006).Actinocatenispora thailandica gen. nov., sp. nov., a new member of the family Micromonosporaceae. Int J Syst Evol Microbiol 56, 1789–1794.[CrossRef] [Google Scholar]
  51. Thiemann, J. E., Pagani, H. & Beretta, G.(1967). A new genus of the Actinoplanaceae: Dactylosporangium gen. nov. Arch Mikrobiol 58, 42–52.[CrossRef] [Google Scholar]
  52. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  53. Tomiyasu, I.(1982). Mycolic acid composition and thermally adaptative changes in Nocardia asteroides. J Bacteriol 151, 828–837. [Google Scholar]
  54. Uchida, K. & Aida, K.(1984). An improved method for the glycolate test for simple identification of acyl type of bacterial cell walls. J Gen Appl Microbiol 30, 131–134.[CrossRef] [Google Scholar]
  55. Vobis, G.(1987). Actinoplanetes. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2418–2419. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  56. Waksman, S. A.(1950).The Actinomycetes: Their Nature, Occurrence, Activities and Importance. Waltham, MA: Chronica Botanica.
  57. Waksman, S. A.(1961).The Actinomycetes, vol. 2, Classification, Identification, and Description of Genera and Species. Baltimore: Williams & Wilkins.
  58. Yokota, A., Tamura, T., Hasegawa, T. & Huang, L. H.(1993).Catenuloplanes japonicus gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales. Int J Syst Bacteriol 43, 805–812.[CrossRef] [Google Scholar]

Data & Media loading...


vol. , part 7, pp. 1644 - 1652

Cultural characteristics of strain 3-44-a(19) .

Physiological characteristics of strain 3-44-a(19) .

Cellular fatty acid composition of strain 3-44-a(19) .

16S rRNA gene sequence similarity (%) of strain 3-44-a(19) and members of closely related genera.

[PDF file of Supplementary Tables S1-S4](56 KB)


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error