1887

Abstract

A Gram-negative, strictly aerobic, rod-shaped bacterium, designated strain BD-c54, was isolated from BTEX (benzene, toluene, ethylbenzene and xylenes)-contaminated soil in Sacheon, Korea. Growth of strain BD-c54 was observed at 15–35 °C (optimum 25–30 °C) and pH 6.0–9.5 (optimum pH 7.0–8.0). The predominant fatty acids were iso-C, iso-C 9, iso-C 3-OH, iso-C, iso-C and iso-C. The strain contained large amounts of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol and a small amount of an unknown amino-group-containing polar lipid as polar lipids. The major quinone was ubiquinone-8 (Q-8) and the G+C content of the genomic DNA was 67.5 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain BD-c54 formed a tight phylogenetic lineage with GR12-1 within the genus and was most closely related to GR12-1 and [] DS-16, with 98.3 and 96.6 % 16S rRNA gene sequence similarity, respectively. The DNA–DNA relatedness between strain BD-c54 and GR12-1 was 24.5 %. On the basis of chemotaxonomic data and molecular properties, strain BD-c54 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is BD-c54 (=KCTC 22080 =DSM 19373). In addition, the transfer of to as comb. nov. and an emended description of the genus are proposed.

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2008-09-01
2019-10-14
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References

  1. Assih, E. A., Ouattara, A. S., Thierry, S., Cayol, J.-L., Labat, M. & Macarie, H. ( 2002; ). Stenotrophomonas acidaminiphila sp. nov., a strictly aerobic bacterium isolated from an upflow anaerobic sludge blanket (UASB) reactor. Int J Syst Evol Microbiol 52, 559–568.
    [Google Scholar]
  2. Batrakov, S. G., Nikitin, D. I., Sheichenko, V. I. & Ruzhitsky, A. O. ( 1997; ). Unusual lipid composition of the gram-negative, freshwater, stalked bacterium Caulobacter bacteroides NP-105. Biochim Biophys Acta 1347, 127–139.[CrossRef]
    [Google Scholar]
  3. Chang, J. S., Chou, C. L., Lin, G. H., Sheu, S. Y. & Chen, W. M. ( 2005; ). Pseudoxanthomonas kaohsiungensis, sp. nov., a novel bacterium isolated from oil-polluted site produces extracellular surface activity. Syst Appl Microbiol 28, 137–144.[CrossRef]
    [Google Scholar]
  4. Chen, M.-Y., Tsay, S.-S., Chen, K.-Y., Shi, Y.-C., Lin, Y.-T. & Lin, G.-H. ( 2002; ). Pseudoxanthomonas taiwanensis sp. nov., a novel thermophilic, N2O-producing species isolated from hot springs. Int J Syst Evol Microbiol 52, 2155–2161.[CrossRef]
    [Google Scholar]
  5. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 2002; ). phylip (phylogeny inference package) version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  7. Finkmann, W., Altendorf, K., Stackebrandt, E. & Lipski, A. ( 2000; ). Characterization of N2O-producing Xanthomonas-like isolates from biofilters as Stenotrophomonas nitritireducens sp. nov., Luteimonas mephitis gen. nov., sp. nov. and Pseudoxanthomonas broegbernensis gen. nov., sp. nov. Int J Syst Evol Microbiol 50, 273–282.[CrossRef]
    [Google Scholar]
  8. Gomori, G. ( 1955; ). Preparation of buffers for use in enzyme studies. Methods Enzymol 1, 138–146.
    [Google Scholar]
  9. Harada, R. M., Campbell, S. & Li, Q. X. ( 2006; ). Pseudoxanthomonas kalamensis sp. nov., a novel gammaproteobacterium isolated from Johnston Atoll, North Pacific Ocean. Int J Syst Evol Microbiol 56, 1103–1107.[CrossRef]
    [Google Scholar]
  10. Jeon, C. O., Park, W., Ghiorse, W. C. & Madsen, E. L. ( 2004; ). Polaromonas naphthalenivorans sp. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment. Int J Syst Evol Microbiol 54, 93–97.[CrossRef]
    [Google Scholar]
  11. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  12. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  13. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  14. Lányí, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  15. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  16. Rosselló-Mora, R. & Amann, R. ( 2001; ). The species concept for prokaryotes. FEMS Microbiol Rev 25, 39–67.[CrossRef]
    [Google Scholar]
  17. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  18. Stanier, R. Y., Palleroni, N. J. & Doudoroff, M. ( 1966; ). The aerobic pseudomonads: a taxonomic study. J Gen Microbiol 43, 159–271.[CrossRef]
    [Google Scholar]
  19. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  20. Thierry, S., Macarie, H., Iizuka, T., Geißdörfer, W., Assih, E. A., Spanevello, M., Verhe, F., Thomas, P., Fudou, R. & other authors ( 2004; ). Pseudoxanthomonas mexicana sp. nov. and Pseudoxanthomonas japonensis sp. nov., isolated from diverse environments, and emended descriptions of the genus Pseudoxanthomonas Finkmann et al. 2000 and of its type species. Int J Syst Evol Microbiol 54, 2245–2255.[CrossRef]
    [Google Scholar]
  21. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  22. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  23. Weon, H. Y., Kim, B. Y., Kim, J. S., Lee, S. Y., Cho, Y. H., Go, S. J., Hong, S. B., Im, W. T. & Kwon, S. W. ( 2006; ). Pseudoxanthomonas suwonensis sp. nov., isolated from cotton waste composts. Int J Syst Evol Microbiol 56, 659–662.[CrossRef]
    [Google Scholar]
  24. Yang, D.-C., Im, W.-T., Kim, M. K. & Lee, S.-T. ( 2005; ). Pseudoxanthomonas koreensis sp. nov. and Pseudoxanthomonas daejeonensis sp. nov. Int J Syst Evol Microbiol 55, 787–791.[CrossRef]
    [Google Scholar]
  25. Yoo, S.-H., Weon, H.-Y., Kim, B.-Y., Kim, J.-H., Baek, Y.-K., Kwon, S.-W., Go, S.-J. & Stackebrandt, E. ( 2007; ). Pseudoxanthomonas yeongjuensis sp. nov., isolated from soil cultivated with Korean ginseng. Int J Syst Evol Microbiol 57, 646–649.[CrossRef]
    [Google Scholar]
  26. Yoon, J. H., Kang, S. J., Oh, H. W. & Oh, T. K. ( 2006; ). Stenotrophomonas dokdonensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 56, 1363–1367.[CrossRef]
    [Google Scholar]
  27. Young, C. C., Ho, M.-J., Arun, A. B., Chen, W.-M., Lai, W.-A., Shen, F.-T., Rekha, P. D. & Yassin, A. F. ( 2007; ). Pseudoxanthomonas spadix sp. nov., isolated from oil-contaminated soil. Int J Syst Evol Microbiol 57, 1823–1827.[CrossRef]
    [Google Scholar]
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Transmission electron micrograph of cells of strain BD-c54 . [PDF](23 KB)

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Results of TLC of the polar lipids of strain BD-c54 . [PDF](22 KB)

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