A Gram-negative, strictly aerobic, rod-shaped bacterium, designated strain BD-c54, was isolated from BTEX (benzene, toluene, ethylbenzene and xylenes)-contaminated soil in Sacheon, Korea. Growth of strain BD-c54 was observed at 15–35 °C (optimum 25–30 °C) and pH 6.0–9.5 (optimum pH 7.0–8.0). The predominant fatty acids were iso-C, iso-C 9, iso-C 3-OH, iso-C, iso-C and iso-C. The strain contained large amounts of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol and a small amount of an unknown amino-group-containing polar lipid as polar lipids. The major quinone was ubiquinone-8 (Q-8) and the G+C content of the genomic DNA was 67.5 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain BD-c54 formed a tight phylogenetic lineage with GR12-1 within the genus and was most closely related to GR12-1 and [] DS-16, with 98.3 and 96.6 % 16S rRNA gene sequence similarity, respectively. The DNA–DNA relatedness between strain BD-c54 and GR12-1 was 24.5 %. On the basis of chemotaxonomic data and molecular properties, strain BD-c54 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is BD-c54 (=KCTC 22080 =DSM 19373). In addition, the transfer of to as comb. nov. and an emended description of the genus are proposed.


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Transmission electron micrograph of cells of strain BD-c54 . [PDF](23 KB)


Results of TLC of the polar lipids of strain BD-c54 . [PDF](22 KB)


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