1887

Abstract

The taxonomic position of five gene clusters of complex (Bcc) isolates was determined using a polyphasic taxonomic approach. The levels of 16S rRNA and gene sequence similarity, multilocus sequence typing (MLST) data and the intermediate DNA–DNA binding values demonstrated that these five clusters represented five novel species within the Bcc. Biochemical identification of these species is difficult, as is the case for most Bcc species. However, identification of these novel species can be accomplished through gene sequence analysis, MLST and BOX-PCR profiling and by RFLP analysis. For diagnostic laboratories, gene sequence analysis offers the best combination of accuracy and simplicity. Based on these results, we propose five novel Bcc species, sp. nov. (type strain FIRENZE 3 =LMG 24064 =CCUG 54555), sp. nov. (type strain AU1075 =LMG 24065 =CCUG 54558), sp. nov. (type strain ES0263A =LMG 24066 =CCUG 54561), sp. nov. (type strain AU0475 =LMG 24067 =CCUG 54564) and sp. nov. (type strain AU0553 =LMG 24068 =CCUG 54567). In the present study, we also demonstrate that should be considered a member of the Bcc.

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2008-07-01
2019-08-20
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vol. , part 7, pp. 1580 - 1590

DNA base ratios and DNA–DNA binding values of all strains examined.

III/ I gene RFLP patterns observed in clusters 1–5.

Dendrogram derived from UPGMA linkage of correlation coefficients between BOX-PCR fingerprint patterns from Bcc isolates.

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