1887

Abstract

Two yellow-pigmented, Gram-negative, aerobic bacterial strains, designated FR1087 and FR1093, were isolated from surface seawater off Jeju Island, Republic of Korea. Both strains required sea salts for growth. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two isolates belong to the genus , showing the highest level of sequence similarity with respect to SW-151 (97.9 %). The two isolates shared 98.5 % sequence similarity. DNA–DNA hybridization between the isolates and the type strain of clearly suggested that strains FR1087 and FR1093 represent two separate novel species in the genus . Several phenotypic characteristics served to differentiate these two isolates from recognized members of the genus . The data from the polyphasic study presented here indicated that strains FR1087 and FR1093 should be classified as representing novel species in the genus , for which the names sp. nov. and sp. nov., respectively, are proposed. The type strain of sp. nov. is FR1087 (=IMSNU 14132=KCTC 12763=JCM 14161) and the type strain of sp. nov. is FR1093 (=IMSNU 14133=KCTC 12764=JCM 14162).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65614-0
2008-10-01
2020-01-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/10/2415.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65614-0&mimeType=html&fmt=ahah

References

  1. Busse, H., El-Banna, T. & Auling, G. ( 1989; ). Evaluation of different approaches for identification of xenobiotic-degrading pseudomonads. Appl Environ Microbiol 55, 1578–1583.
    [Google Scholar]
  2. Busse, H.-J., Denner, E. B. M., Buczolits, S., Salkinoja-Salonen, M., Bennasar, A. & Kampfer, P. ( 2003; ). Sphingomonas aurantiaca sp. nov., Sphingomonas aerolata sp. nov. and Sphingomonas faeni sp. nov., air- and dustborne and Antarctic, orange-pigmented, psychrotolerant bacteria, and emended description of the genus Sphingomonas. Int J Syst Evol Microbiol 53, 1253–1260.[CrossRef]
    [Google Scholar]
  3. Chun, J. & Goodfellow, M. ( 1995; ). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45, 240–245.[CrossRef]
    [Google Scholar]
  4. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y.-W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  5. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  6. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  8. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  9. Flores, H. E. & Galston, A. W. ( 1982; ). Analysis of polyamines in higher plants by high performance liquid chromatography. Plant Physiol 69, 701–706.[CrossRef]
    [Google Scholar]
  10. Godoy, F., Vancanneyt, M., Martinez, M., Steinbuchel, A., Swings, J. & Rehm, B. H. ( 2003; ). Sphingopyxis chilensis sp. nov., a chlorophenol-degrading bacterium that accumulates polyhydroxyalkanoate, and transfer of Sphingomonas alaskensis to Sphingopyxis alaskensis comb. nov. Int J Syst Evol Microbiol 53, 473–477.[CrossRef]
    [Google Scholar]
  11. Jeon, Y. S., Chung, H., Park, S., Hur, I., Lee, J. H. & Chun, J. ( 2005; ). jPHYDIT: a JAVA-based integrated environment for molecular phylogeny of ribosomal RNA sequences. Bioinformatics 21, 3171–3173.[CrossRef]
    [Google Scholar]
  12. Kämpfer, P., Witzenberger, R., Denner, E. B. M., Busse, H.-J. & Neef, A. ( 2002; ). Sphingopyxis witflariensis sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 52, 2029–2034.[CrossRef]
    [Google Scholar]
  13. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  14. Lee, J.-S., Shin, Y. K., Yoon, J.-H., Takeuchi, M., Pyun, Y.-R. & Park, Y.-H. ( 2001; ). Sphingomonas aquatilis sp. nov., Sphingomonas koreensis sp. nov. and Sphingomonas taejonensis sp. nov., yellow-pigmented bacteria isolated from natural mineral water. Int J Syst Evol Microbiol 51, 1491–1498.
    [Google Scholar]
  15. Lyman, J. & Fleming, R. H. ( 1940; ). Composition of sea water. J Mar Res 3, 134–146.
    [Google Scholar]
  16. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  17. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  18. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  19. Swofford, D. L. ( 1998; ). paup: Phylogenetic analysis using parsimony, version 4. Sunderland, MA: Sinauer Associates.
  20. Takeuchi, M., Kawai, F., Shimada, Y. & Yokota, A. ( 1993; ). Taxonomic study of polyethylene glycol-utilizing bacteria: emended description of the genus Sphingomonas and new descriptions of Sphingomonas macrogoltabidus sp. nov., Sphingomonas sanguis sp. nov. and Sphingomonas terrae sp. nov. Syst Appl Microbiol 16, 227–238.[CrossRef]
    [Google Scholar]
  21. Takeuchi, M., Hamana, K. & Hiraishi, A. ( 2001; ). Proposal of the genus Sphingomonas sensu stricto and three new genera, Sphingobium, Novosphingobium and Sphingopyxis, on the basis of phylogenetic and chemotaxonomic analyses. Int J Syst Evol Microbiol 51, 1405–1417.
    [Google Scholar]
  22. Vancanneyt, M., Schut, F., Snauwaert, C., Goris, J., Swings, J. & Gottschal, J. C. ( 2001; ). Sphingomonas alaskensis sp. nov., a dominant bacterium from a marine oligotrophic environment. Int J Syst Evol Microbiol 51, 73–79.
    [Google Scholar]
  23. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  24. Yabuuchi, E., Kosako, Y., Fujiwara, N., Naka, T., Matsunaga, I., Ogura, H. & Kobayashi, K. ( 2002; ). Emendation of the genus Sphingomonas Yabuuchi et al. 1990 and junior objective synonymy of the species of three genera, Sphingobium, Novosphingobium and Sphingopyxis, in conjunction with Blastomonas ursincola. Int J Syst Evol Microbiol 52, 1485–1496.[CrossRef]
    [Google Scholar]
  25. Yi, H. & Chun, J. ( 2006; ). Thalassobius aestuarii sp. nov., isolated from tidal flat sediment. J Microbiol 44, 171–176.
    [Google Scholar]
  26. Yi, H., Oh, H.-M., Lee, J.-H., Kim, S.-J. & Chun, J. ( 2005; ). Flavobacterium antarcticum sp. nov., a novel psychrotolerant bacterium isolated from the Antarctic. Int J Syst Evol Microbiol 55, 637–641.[CrossRef]
    [Google Scholar]
  27. Yoon, J.-H. & Oh, T.-K. ( 2005; ). Sphingopyxis flavimaris sp. nov., isolated from sea water of the Yellow Sea in Korea. Int J Syst Evol Microbiol 55, 369–373.[CrossRef]
    [Google Scholar]
  28. Yoon, J.-H., Lee, C.-H., Yeo, S.-H. & Oh, T.-K. ( 2005; ). Sphingopyxis baekryungensis sp. nov., an orange-pigmented bacterium isolated from sea water of the Yellow Sea in Korea. Int J Syst Evol Microbiol 55, 1223–1227.[CrossRef]
    [Google Scholar]
  29. ZoBell, C. E. ( 1941; ). Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 4, 42–75.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65614-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65614-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error