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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 58, Issue 10

sp. nov., a spore-forming lactic acid bacterium isolated from vineyard soil Free

Abstract

Two spore-forming, facultatively anaerobic, lactic acid bacteria, strains SL153 and SL1153, were isolated from vineyard soil in Korea. Cells of both strains were slightly curved, Gram-positive, motile rods that measured between 1 and 4 μm in length and were approximately 0.5 μm in diameter. Strains SL153 and SL1153 fermented glucose, fructose, mannose and sorbitol, but were negative for nitrate reduction, catalase and oxidase. The predominant cellular fatty acids of the two isolates were iso-C, anteiso-C and anteiso-C. Diaminopimelic acid, glucose, mannose and galactose were determined in their whole-cell hydrolysates. 16S rRNA gene sequences from the two strains were almost identical (99.9 %) and they could be placed in the genus according to phylogenetic analysis. The species most closely related to SL153 were and with 16S rRNA gene similarities of 95.7 and 95.5 %, respectively, with the type strains. Levels of DNA–DNA relatedness between strain SL153 and the type strains of , and were 18.5, 18.0 and 17.0 %, respectively. On the basis of the phylogenetic (16S rRNA gene), chemotaxonomic and phenotypic evidence given in this study, it is proposed that strains SL153 and SL1153 should be assigned to the genus as representatives of the novel species sp. nov. The type strain is SL153 (=KCTC 5376=JCM 14637).

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2008-10-01
2025-12-05

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References

  1. Chang, Y.-H., Kim, J.-K., Kim, H.-J., Kim, W.-Y., Kim, Y.-B. & Park, Y.-H.(2001). Selection of a potential probiotic Lactobacillus strain and subsequent in vivo studies. Antonie van Leeuwenhoek 80, 193–199.[CrossRef] [Google Scholar]
  2. Chang, Y.-H., Han, J.-I., Chun, J., Lee, K. C., Rhee, M.-S., Kim, Y.-B. & Bae, K. S.(2002).Comamonas koreensis sp. nov., a non-motile species from wetland in Woopo, Korea. Int J Syst Evol Microbiol 52, 377–381. [Google Scholar]
  3. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  4. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  5. Felsenstein, J.(1993).phylip (phylogeny inference package) version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  6. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  7. Fitch, W. M. & Margoliash, E.(1967). Construction of phylogenetic trees. Science 155, 279–284.[CrossRef] [Google Scholar]
  8. Garrity, G. M. & Holt, J. G.(2001). The road map to the Manual. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 119–166. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer
  9. Hatayama, K., Shoun, H., Ueda, Y. & Nakamura, A.(2006).Tuberibacillus calidus gen. nov., sp. nov., isolated from a compost pile and reclassification of Bacillus naganoensis Tomimura et al. 1990 as Pullulanibacillus naganoensis gen. nov., comb. nov. and Bacillus laevolacticus Andersch et al. 1994 as Sporolactobacillus laevolacticus comb. nov. Int J Syst Evol Microbiol 56, 2545–2551.[CrossRef] [Google Scholar]
  10. Holzapfel, W. H. & Botha, S. J.(1988). Physiology of Sporolactobacillus strains isolated from different habitats and the indication of in vitro antagonism against Bacillus species. Int J Food Microbiol 7, 161–168.[CrossRef] [Google Scholar]
  11. Huang, H.-Y., Huang, S.-Y., Chen, P.-Y., King, V.-A., Lin, Y.-P. & Tsen, J.-H.(2007). Basic characteristics of Sporolactobacillus inulinus BCRC 14647 for potential probiotic properties. Curr Microbiol 54, 396–404.[CrossRef] [Google Scholar]
  12. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  13. Kitahara, K. & Suzuki, J.(1963).Sporolactobacillus nov. subgen. J Gen Appl Microbiol 9, 59–71.[CrossRef] [Google Scholar]
  14. Kitahara, K. & Toyota, T.(1972). Auto-spheroplastization and cell-permeation in Sporolactobacillus inulinus. J Gen Appl Microbiol 18, 99–107.[CrossRef] [Google Scholar]
  15. Maidak, B. L., Cole, J. R., Lilburn, T. G., Parker, C. T., Jr, Saxman, P. R., Farris, R. J., Garrity, G. M., Olsen, G. J., Schmidt, T. M. & Tiedje, J. M.(2001). The RDP-II (Ribosomal Database Project). Nucleic Acids Res 29, 173–174.[CrossRef] [Google Scholar]
  16. Mandel, M. & Marmur, J.(1968). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B, 195–206. [Google Scholar]
  17. Nakayama, O. & Yanoshi, M.(1967a). Spore-bearing lactic acid bacteria isolated from rhizosphere. II. Taxonomic studies on the catalase-negative strains. J Gen Appl Microbiol 13, 155–165.[CrossRef] [Google Scholar]
  18. Nakayama, O. & Yanoshi, M.(1967b). Spore-bearing lactic acid bacteria isolated from rhizosphere. I. Taxonomic studies on Bacillus laevolacticus nov. sp. and Bacillus racemilacticus nov. sp. J Gen Appl Microbiol 13, 139–153.[CrossRef] [Google Scholar]
  19. Rhuland, L. E., Work, E., Denman, R. F. & Hoare, D. S.(1955). The behavior of the isomers of α,ϵ-diaminopimelic acid on paper chromatograms. J Am Chem Soc 77, 4844–4846.[CrossRef] [Google Scholar]
  20. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  21. Schleifer, K. H. & Kandler, O.(1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477. [Google Scholar]
  22. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  23. Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231. [Google Scholar]
  24. Yanagida, F., Suzuki, K.-I., Kaneko, T., Kozaki, M. & Komagata, K.(1987). Morphological, biochemical, and physiological characteristics of spore-forming lactic acid bacteria. J Gen Appl Microbiol 33, 33–45.[CrossRef] [Google Scholar]
  25. Yanagida, F., Suzuki, K.-I., Kozaki, M. & Komagata, K.(1997). Proposal of Sporolactobacillus nakayamae subsp. nakayamae sp. nov., subsp. nov., Sporolactobacillus nakayamae subsp. racemicus subsp. nov., Sporolactobacillus terrae sp. nov., Sporolactobacillus kofuensis sp. nov., and Sporolactobacillus lactosus sp. nov. Int J Syst Bacteriol 47, 499–504.[CrossRef] [Google Scholar]
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Sporolactobacillus vineae sp. nov., a spore-forming lactic acid bacterium isolated from vineyard soil
Int J Syst Evol Microbiol 58, 2316 (2008); https://doi.org/10.1099/ijs.0.65608-0
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vol. , part 10, pp. 2316 - 2320

Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showing the position of strains SL153 and SL1153 ( sp. nov.) and closely related strains of the family ' '. [PDF](15 KB)

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