A marine bacterial strain, designated strain JUB59, was isolated from surface seawater in Antarctica and subsequently characterized. Cells were found to be Gram-negative, non-motile rods forming butyrous, shiny, yellowish orange colonies on marine agar. Growth occurred at 2–28 °C (optimally at 22–25 °C) but not at 30 °C; Na ions were required, but 9 % NaCl (w/v) was not tolerated. Phylogenetic analysis, based on comparisons of the complete 16S rRNA gene sequence of the novel isolate with the sequences of closely related strains, showed that strain JUB59 belonged to the family , representing a novel species of the genus . The highest levels of sequence similarity were found with respect to ADA-4 (97.4 %) and APA-1 (97.1 %). However, the DNA–DNA relatedness of strain JUB59 with respect to these two strains was low (15.9–17.3  and 19.3–22.1 %, respectively). The predominant fatty acids of strain JUB59 were iso-15 : 110 (18.1 %), iso-15 : 0 (17.3 %), anteiso-15 : 0 (13.9 %), iso-17 : 0 3-OH (9.2 %), 15 : 0 (6.0 %) and iso-16 : 0 3-OH (5.3 %). The main polar lipids were phosphatidylethanolamine, an aminolipid, an amino-positive phospholipid and two unidentified lipids. MK-6 was the major respiratory quinone (>90 %) and the DNA G+C content was 34 mol%. On the basis of the data obtained, strain JUB59 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JUB59 (=DSM 19628=CCM-A-29 1259).


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Cellular fatty acid composition (%) of strain JUB59 and the type strains of recognized species of the genus . [ PDF] 103 KB

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