Four novel strains of budding bacteria, designated MOB10, PO2, MPL1015 and BG32, were isolated from acidic wetlands of northern Russia. Cells of these four strains were aerobic, non-motile spheres that occurred singly or in shapeless aggregates and attached to surfaces by means of a holdfast material. The isolates were moderately acidophilic, mesophilic organisms capable of growth between pH 4.2 and 7.5 (optimum growth at pH 5.0–6.2) and at temperatures between 4 and 33 °C (optimum growth at 20–26 °C). The strains possessed a complex intracellular membrane system that compartmentalized the cells. The major fatty acids were C, C 9 and C 6,12. The major quinone was menaquinone-6 (MK-6). The G+C content of the DNA was 57.8–59.9 mol%. 16S rRNA gene sequence analysis showed that strains MOB10, PO2, MPL1015 and BG32 were members of the order and belonged to a phylogenetic lineage defined by the genus , exhibiting 90 % sequence similarity to the type strain of the thermophilic planctomycete and 95–95.5 % sequence similarity to a taxonomically uncharacterized group of filamentous bacteria from activated sludge, ‘’ III. However, compared with ‘’ III and , the new isolates from acidic wetlands were non-filamentous, unpigmented bacteria, which possessed highly distinctive phospholipid fatty acid profiles and were capable of growth and of degrading several biopolymers under acidic, microaerobic and cold conditions. The data suggest that the four isolates should be considered as representing a novel species of a new genus of the order , for which the name gen. nov., sp. nov. is proposed. The type strain of is MOB10 (=ATCC BAA-1392 =VKM B-2454 =DSM 18658).


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vol. , part 5, pp. 1186 - 1193

Influence of medium pH on the growth of strain MOB10 .


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