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Abstract

A Gram-negative, motile, rod-shaped, extremely halophilic archaeon, designated strain HPC1-2, was isolated from , a salt-fermented fish product of Thailand. Strain HPC1-2 was able to grow at 20–60 °C (optimum at 37–40 °C), at 2.6–5.1 M NaCl (optimum at 3.4–4.3 M NaCl) and at pH 5.0–8.0 (optimum at pH 7.0–7.5). Hypotonic treatment with less than 1.7 M NaCl caused cell lysis. The major polar lipids of the isolate were CC derivatives of phosphatidylglycerol, methylated phosphatidylglycerol phosphate, phosphatidylglycerol sulfate, triglycosyl diether, sulfated triglycosyl diether and sulfated tetraglycosyl diether. The G+C content of the DNA was 65.5 mol%. 16S rRNA gene sequence analysis indicated that the isolate represented a member of the genus in the family . Based on 16S rRNA gene sequence similarity, strain HPC1-2 was related most closely to DSM 3754 (99.2 %) and JCM 13558 (97.8 %). However, low levels of DNA–DNA relatedness suggested that strain HPC1-2 was genotypically different from these closely related type strains. Strain HPC1-2 could also be differentiated based on physiological and biochemical characteristics. Therefore, strain HPC1-2 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HPC1-2 (=BCC 24372=JCM 14661=PCU 302).

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2008-09-01
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vol. , part 9, pp. 2136 - 2140

Maximum-parsimony phylogenetic tree showing the relationships between strain HPC1-2 and related archaeal species based on 16S rRNA gene sequences.

Maximum-likelihood phylogenetic tree showing the relationships between strain HPC1-2 and related archaeal species based on 16S rRNA gene sequences.

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