1887

Abstract

Three Gram-positive, coccoid to rod-shaped actinobacteria, designated strains MSL-09, MSL-19 and MSL-28, were isolated from a soil sample collected from Bigeum Island in Korea, and were subjected to a polyphasic taxonomic analysis. All three isolates grew optimally at pH 7.5–9.0 and 28–30 °C. 16S rRNA gene analysis revealed that all three strains belonged to the genus , with strains MSL-09, MSL-19 and MSL-28 respectively showing the highest sequence similarity to DSM 11439 (96.25 %), GW-9 (95.75 %) and DS-17 (95.61 %). Chemotaxonomically, they contain -2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H) as the predominant menaquinone and diphosphatidylglycerol, phosphatidylglycerol and some unknown lipids as the polar lipids found in the cell wall. iso-C is a major fatty acid. The G+C content of the DNA was respectively 69.9, 69.3 and 69.4 mol% for strains MSL-09, MSL-19 and MSL-28. Based on morphological, physiological, biochemical and chemotaxonomic characters presented in this study, the three strains represent novel species of the genus . The names sp. nov. (type strain MSL-09 =KCTC 19272 =DSM 19266), sp. nov. (type strain MSL-19 =KCTC 19290 =DSM 19320) and sp. nov. (type strain MSL-28 =KCTC 19276 =DSM 19323) are proposed.

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2008-10-01
2019-10-23
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Scanning electron micrographs of cells of strains MSL-09 (a), MSL-19 (b) and MSL-28 (c) grown on R2A agar for 7 days at 28 °C. Bars, 2 µm (a) and 1 µm (b, c).

IMAGE

Scanning electron micrographs of cells of strains MSL-09 (a), MSL-19 (b) and MSL-28 (c) grown on R2A agar for 7 days at 28 °C. Bars, 2 µm (a) and 1 µm (b, c).

IMAGE

Scanning electron micrographs of cells of strains MSL-09 (a), MSL-19 (b) and MSL-28 (c) grown on R2A agar for 7 days at 28 °C. Bars, 2 µm (a) and 1 µm (b, c).

IMAGE

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