1887

Abstract

Two 4-chlorophenol-degrading bacteria, strains 4C1-a and 4C1-b, were isolated from sewage flowing into Geumho River near the Daegu industrial complex in Korea. Cells of the strains were Gram-positive and non-motile, displayed a rod–coccus life cycle and formed creamy white colonies on R2A agar or peptone-carbohydrate agar. The strains had chemotaxonomic markers that were consistent with classification in the genus , i.e. MK-9(H) as the major menaquinone, iso- and anteiso-branched components as the predominant fatty acids, galactose, glucose and rhamnose as cell-wall sugars, peptidoglycan-type A3, and DNA G+C content of 63.5–64.4 mol%. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that the strains were most similar to DSM 12829, DSM 20119 and JCM 12642 (with 98.4, 97.8 and 97.8 % similarity, respectively) and formed a separate lineage with in the genus . Combined phenotypic data and DNA–DNA hybridization data supported the conclusion that strains 4C1-a and 4C1-b represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is 4C1-a (=KCTC 19209=DSM 18782).

Keyword(s): 4-CP, 4-chlorophenol
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2008-08-01
2021-06-23
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References

  1. Bates, R. G. & Bower, V. E.(1956). Alkaline solutions for pH control. Anal Chem 28, 1322–1324.[CrossRef] [Google Scholar]
  2. Borodina, E., Kelly, D. P., Schumann, P., Rainey, F. A., Ward-Rainey, N. L. & Wood, A. P.(2002). Enzymes of dimethylsulfone metabolism and the phylogenetic characterization of the facultative methylotrophs Arthrobacter sulfonivorans sp. nov., Arthrobacter methylotrophus sp. nov., and Hyphomicrobium sulfonivorans sp. nov. Arch Microbiol 177, 173–183.[CrossRef] [Google Scholar]
  3. Conn, H. J. & Dimmick, I.(1947). Soil bacteria similar in morphology to Mycobacterium and Corynebacterium. J Bacteriol 54, 291–303. [Google Scholar]
  4. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  5. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  6. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  7. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors)(1994).Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  8. Gomori, G.(1955). Preparation of buffers for use in enzyme studies. Methods Enzymol 1, 138–146. [Google Scholar]
  9. Hall, T. A.(1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98. [Google Scholar]
  10. Huang, Y., Zhao, N., He, L., Wang, L., Liu, Z., You, M. & Guan, F.(2005).Arthrobacter scleromae sp. nov. isolated from human clinical specimens. J Clin Microbiol 43, 1451–1455.[CrossRef] [Google Scholar]
  11. Ishikawa, T. & Yokota, A.(2006). Reclassification of Arthrobacter duodecadis Lochhead 1958 as Tetrasphaera duodecadis comb. nov. and emended description of the genus Tetrasphaera. Int J Syst Evol Microbiol 56, 1369–1373.[CrossRef] [Google Scholar]
  12. Jones, D. & Keddie, R. M.(1992). The genus Arthrobacter. In The Prokaryotes: a Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2nd edn, vol. 2, pp. 1283–1299. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  13. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  14. Kageyama, A., Takahashi, Y., Morisaki, K. & Omura, S.(2008).Arthrobacter oryzae sp. nov. and Arthrobacter humicola sp. nov. Int J Syst Evol Microbiol 58, 53–56.[CrossRef] [Google Scholar]
  15. Kämpfer, P. & Kroppenstedt, R. M.(1996). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef] [Google Scholar]
  16. Keddie, R. M., Collins, M. D. & Jones, D.(1986). Genus Arthrobacter Conn and Dimmick 1947, 300AL. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1288–1301. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  17. Klatte, S., Rainey, F. A. & Kroppenstedt, R. M.(1994). Transfer of Rhodococcus aichiensis Tsukamura 1982 and Nocardia amarae Lechevalier & Lechevalier 1974 to the genus Gordona as Gordona aichiensis comb. nov. and Gordona amarae comb. nov. Int J Syst Bacteriol 44, 769–773.[CrossRef] [Google Scholar]
  18. Koch, C., Schumann, P. & Stackebrandt, E.(1995). Reclassification of Micrococcus agilis (Ali-Cohen 1889) to the genus Arthrobacter as Arthrobacter agilis comb. nov. and emendation of the genus Arthrobacter. Int J Syst Bacteriol 45, 837–839.[CrossRef] [Google Scholar]
  19. Kodama, Y., Yamamoto, H., Amano, N. & Amachi, T.(1992). Reclassification of two strains of Arthrobacter oxydans and proposal of Arthrobacter nicotinovorans sp. nov. Int J Syst Bacteriol 42, 234–239.[CrossRef] [Google Scholar]
  20. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M.(2001).mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef] [Google Scholar]
  21. Miller, J. H.(1972).Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
  22. Mudarris, M., Austin, B., Segers, P., Vancanneyt, M., Hoste, B. & Bernardet, J. F.(1994).Flavobacterium scophthalmum sp. nov., a pathogen of turbot (Scophthalmus maximus L.). Int J Syst Bacteriol 44, 447–453.[CrossRef] [Google Scholar]
  23. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E.(1996). The genus Nocardiopsis represents a phylogenetically coherent taxon and a district actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef] [Google Scholar]
  24. Reddy, G. S. N., Prakash, J. S. S., Matsumoto, G. I., Stackebrandt, E. & Shivaji, S.(2002).Arthrobacter roseus sp. nov., a psychrophilic bacterium isolated from an Antarctic cyanobacterial mat sample. Int J Syst Evol Microbiol 52, 1017–1021.[CrossRef] [Google Scholar]
  25. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  26. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  27. Schleifer, K. H. & Kandler, O.(1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477. [Google Scholar]
  28. Shin, Y. K., Lee, J.-S., Chun, C. O., Kim, H.-J. & Park, Y.-H.(1996). Isoprenoid quinone profiles of the Leclercia adecarboxylata KCTC 1036T. J Microbiol Biotechnol 6, 68–69. [Google Scholar]
  29. Skerman, V. B. D.(1967).A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins.
  30. Stackebrandt, E., Fowler, V. J., Fiedler, F. & Seiler, H.(1983). Taxonomic studies on Arthrobacter nicotianae and related taxa: description of Arthrobacter uratoxydans sp. nov. and Arthrobacter sulfureus sp. nov. and reclassification of Brevibacterium protophormiae as Arthrobacter protophormiae comb. nov. Syst Appl Microbiol 4, 470–486.[CrossRef] [Google Scholar]
  31. Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231. [Google Scholar]
  32. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phased high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  33. Tarrand, J. J. & Groschel, D. H. M.(1982). Rapid, modified oxidase test for oxidase-variable bacterial isolates. J Clin Microbiol 16, 772–774. [Google Scholar]
  34. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  35. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  36. Westerberg, K., Elväng, A. M., Stackebrandt, E. & Jansson, J. K.(2000).Arthrobacter chlorophenolicus sp. nov., a new species capable of degrading high concentrations of 4-chlorophenol. Int J Syst Evol Microbiol 50, 2083–2092.[CrossRef] [Google Scholar]
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vol. , part 8, pp. 1916 - 1921

Extended neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showing the positions of strains 4C1-a and 4C1-b ( sp. nov.) and recognized species. [PDF](22 KB)



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