1887

Abstract

A moderately alkaliphilic and halophilic bacterium was isolated from sediment of Xiarinaoer soda lake located in the Inner Mongolia municipality. This bacterium, designated strain H-5, was a facultative anaerobe, Gram-positive, rod-shaped and non-motile. Strain H-5 grew in complex medium with 0.5–30 % (w/v) NaCl and at pH 7.5–13. The cell wall peptidoglycan contained -diaminopimelic acid. The major isoprenoid quinones found in this strain were MK-9H and MK-9H, and the major cellular fatty acids were C and anteiso-C. The DNA G+C content of strain H-5 was 38.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain H-5 was located in the genus . The 16S rRNA gene sequence similarities between strain H-5 and the type strains of the two recognized species of the genus were 98.6 and 98.0 %. The DNA–DNA relatedness values between strain H-5 and the two type strains were 19 and 5 %. Based on the phenotypic and chemotaxonomic data, the phylogenetic analysis and genomic distinctiveness, strain H-5 is considered to represent a novel species of the genus , for which the name is proposed. The type strain is H-5 (=CGMCC AS 1.6843=NBRC 103919).

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2008-09-01
2019-10-22
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vol. , part 9, pp. 2169 - 2173

Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences. [PDF](15 KB)



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