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Abstract
A moderately alkaliphilic and halophilic bacterium was isolated from sediment of Xiarinaoer soda lake located in the Inner Mongolia municipality. This bacterium, designated strain H-5T, was a facultative anaerobe, Gram-positive, rod-shaped and non-motile. Strain H-5T grew in complex medium with 0.5–30 % (w/v) NaCl and at pH 7.5–13. The cell wall peptidoglycan contained meso-diaminopimelic acid. The major isoprenoid quinones found in this strain were MK-9H4 and MK-9H2, and the major cellular fatty acids were C16 : 0 and anteiso-C13 : 0. The DNA G+C content of strain H-5T was 38.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain H-5T was located in the genus Halolactibacillus. The 16S rRNA gene sequence similarities between strain H-5T and the type strains of the two recognized species of the genus Halolactibacillus were 98.6 and 98.0 %. The DNA–DNA relatedness values between strain H-5T and the two type strains were 19 and 5 %. Based on the phenotypic and chemotaxonomic data, the phylogenetic analysis and genomic distinctiveness, strain H-5T is considered to represent a novel species of the genus Halolactibacillus, for which the name Halolactibacillus alkaliphilus is proposed. The type strain is H-5T (=CGMCC AS 1.6843T=NBRC 103919T).
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