1887

Abstract

A Gram-negative, non-spore-forming bacterium, designated strain BD-d46, was isolated from a playground soil sample in Jinju, South Korea. Cells were straight or curved rods and showed catalase- and oxidase-positive reactions. Growth of strain BD-d46 was observed between 15 and 35 °C (optimum 25–30 °C) and between pH 6.5 and 8.0 (optimum pH 7.0–7.5). The predominant fatty acids were summed feature 3 (C 7 and/or iso-C 2-OH), C 3-OH and C. Strain BD-d46 contained phosphatidylethanolamine and phosphatidylglycerol as major polar lipids. Isoprenoid quinones were Q-8 (75 %) and MK-7 (25 %). The G+C content of the genomic DNA was 49.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BD-d46 formed a distinct lineage with K19414 within the genus . Levels of 16S rRNA gene sequence similarity between strain BD-d46 and the type strains of recognized species ranged from 94.4 to 96.9 %. On the basis of chemotaxonomic data and molecular properties, strain BD-d46 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BD-d46 (=KCTC 22077 =DSM 19413).

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2008-10-01
2019-10-23
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Transmission electron micrograph showing the general morphology of a negatively stained cell of strain BD-c46 after growth for 3 days at 30 °C in R2A broth. Bar, 1 µm.

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Polar lipid thin-layer chromatograms sprayed with molybdatophosphoric acid reagent of strain BD-d46 (A) and K19414 (B). Ascending solvent systems: (I) chloroform/methanol/water (65:25:4, by vol.); (II) chloroform/acetic acid/methanol/water (80:13:10:2, by vol.). For detection of polar lipids, molybdatophosphoric acid (PE, PG, PL1, AL1, AL2 and AL3), ninhydrin (PE, AL1, AL2 and AL3) and molybdenum blue reagent (PG and PE) were applied. PE, Phosphatidylethanolamine; PG, phosphatidylglycerol; PL1, unknown polar lipid; AL1, AL2 and AL3, unknown polar lipids containing amino groups.

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Cellular fatty acid compositions of strain BD-d46 cultivated on MA and TSA. [PDF](43 KB)

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