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Abstract

A Gram-negative, non-spore-forming bacterium, designated strain BD-d46, was isolated from a playground soil sample in Jinju, South Korea. Cells were straight or curved rods and showed catalase- and oxidase-positive reactions. Growth of strain BD-d46 was observed between 15 and 35 °C (optimum 25–30 °C) and between pH 6.5 and 8.0 (optimum pH 7.0–7.5). The predominant fatty acids were summed feature 3 (C 7 and/or iso-C 2-OH), C 3-OH and C. Strain BD-d46 contained phosphatidylethanolamine and phosphatidylglycerol as major polar lipids. Isoprenoid quinones were Q-8 (75 %) and MK-7 (25 %). The G+C content of the genomic DNA was 49.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BD-d46 formed a distinct lineage with K19414 within the genus . Levels of 16S rRNA gene sequence similarity between strain BD-d46 and the type strains of recognized species ranged from 94.4 to 96.9 %. On the basis of chemotaxonomic data and molecular properties, strain BD-d46 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BD-d46 (=KCTC 22077 =DSM 19413).

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2008-10-01
2024-11-04
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References

  1. Batrakov, S. G., Nikitin, D. I., Sheichenko, V. I. & Ruzhitsky, A. O.(1997). Unusual lipid composition of the gram-negative, freshwater, stalked bacterium Caulobacter bacteroides NP-105. Biochim Biophys Acta 1347, 127–139.[CrossRef] [Google Scholar]
  2. Brettar, I., Christen, R. & Höfle, M. G.(2002).Rheinheimera baltica gen. nov., sp. nov., a blue-coloured bacterium isolated from the central Baltic Sea. Int J Syst Evol Microbiol 52, 1851–1857.[CrossRef] [Google Scholar]
  3. Brettar, I., Christen, R. & Höfle, M. G.(2006).Rheinheimera perlucida sp. nov., a marine bacterium of the Gammaproteobacteria isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 56, 2177–2183.[CrossRef] [Google Scholar]
  4. Felsenstein, J.(2002).phylip (phylogeny inference package), version 3.6a. Department of Genome Sciences, University of Washington, Seattle, USA.
  5. Gomori, G.(1955). Preparation of buffers for use in enzyme studies. Methods Enzymol 1, 138–146. [Google Scholar]
  6. Halpern, M., Senderovich, Y. & Snir, S.(2007).Rheinheimera chironomi sp. nov., isolated from a chironomid (Diptera; Chironomidae) egg mass. Int J Syst Evol Microbiol 57, 1872–1875.[CrossRef] [Google Scholar]
  7. Jeon, C. O., Park, W., Ghiorse, W. C. & Madsen, E. L.(2004).Polaromonas naphthalenivorans sp. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment. Int J Syst Evol Microbiol 54, 93–97.[CrossRef] [Google Scholar]
  8. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  9. Komagata, K. & Suzuki, K.(1987). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19, 161–208. [Google Scholar]
  10. Lane, D. J.(1991). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  11. Lányí, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  12. Leifson, E.(1963). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184. [Google Scholar]
  13. Merchant, M. M., Welsh, K. A. & McLean, J. C. R.(2007).Rheinheimera texasensis sp. nov., a halointolerant freshwater oligotroph. Int J Syst Evol Microbiol 57, 2376–2380.[CrossRef] [Google Scholar]
  14. Romanenko, L. A., Uchino, M., Falsen, E., Zhukova, N. V., Mikhailov, V. V. & Uchimura, T.(2003).Rheinheimera pacifica sp. nov., a novel halotolerant bacterium isolated from deep sea water of the Pacific. Int J Syst Evol Microbiol 53, 1973–1977.[CrossRef] [Google Scholar]
  15. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt. Washington, DC: American Society for Microbiology.
  16. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  17. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  18. Yoon, J.-H., Park, S. E., Kang, S.-J. & Oh, T.-K.(2007).Rheinheimera aquimaris sp. nov., isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 57, 1386–1390.[CrossRef] [Google Scholar]
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Transmission electron micrograph showing the general morphology of a negatively stained cell of strain BD-c46 after growth for 3 days at 30 °C in R2A broth. Bar, 1 µm.

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Polar lipid thin-layer chromatograms sprayed with molybdatophosphoric acid reagent of strain BD-d46 (A) and K19414 (B). Ascending solvent systems: (I) chloroform/methanol/water (65:25:4, by vol.); (II) chloroform/acetic acid/methanol/water (80:13:10:2, by vol.). For detection of polar lipids, molybdatophosphoric acid (PE, PG, PL1, AL1, AL2 and AL3), ninhydrin (PE, AL1, AL2 and AL3) and molybdenum blue reagent (PG and PE) were applied. PE, Phosphatidylethanolamine; PG, phosphatidylglycerol; PL1, unknown polar lipid; AL1, AL2 and AL3, unknown polar lipids containing amino groups.

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Cellular fatty acid compositions of strain BD-d46 cultivated on MA and TSA. [PDF](43 KB)

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