A mycelium-forming actinomycete, designated strain D8-87, was isolated from a soil collected from a cave in Jeju, Republic of Korea. Comparative 16S rRNA gene sequence analysis showed that the organism was closely related to the type and only species of the genus . The level of 16S rRNA gene sequence similarity between strain D8-87 and the type strain of was 99.2 %. The novel organism produced extensively branched aerial and substrate mycelia, which fragmented into short or elongated rod-shaped cells. Whole-cell hydrolysates of the isolate contained -diaminopimelic acid, glucose, mannose and arabinose. The predominant menaquinone was MK-9(H). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannoside and an unknown phospholipid. The predominant fatty acids were anteiso-C, iso-C and iso-C. The DNA G+C content of strain D8-87 was 71.5 mol%. Levels of DNA–DNA relatedness between strain D8-87 and KCTC 19044 were 23.4 and 34.6 %. On the basis of the phenotypic and DNA–DNA hybridization data presented, strain D8-87 is considered to represent a novel species of the genus , for which the name is proposed. The type strain is D8-87 (=JBRI 2008=KCTC 19222=DSM 45079).


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vol. , part 5, pp. 1176 - 1179

Scanning electron micrograph of cells of strain D8-87 grown on ISP 4 medium at 28 °C for 2 weeks.

A neighbour-joining phylogenetic tree showing the phylogenetic relationships of strain D8-87 within the radiation of the order .

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