A taxonomic study was carried out on strain C10-2, a moderately halophilic denitrifier isolated from activated sludge samples collected in China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain C10-2 belonged to the genus , with highest sequence similarity of 98.1 and 97.7 % to ‘’ IAM 12023 and JCM 11590, respectively. The , and gene sequence similarity between strain C10-2 and JCM 11590 was respectively 84.1, 79.0 and 88.2 %. Phylogenetic trees based on these housekeeping genes showed that strain C10-2 and JCM 11590 form a clade at the periphery of the genus . The DNA–DNA hybridization value between strain C10-2 and JCM 11590 was 30–32 %. The G+C content of the chromosomal DNA was 61.2 mol%. The combined genotypic and phenotypic data show that strain C10-2 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain C10-2 (=CGMCC 1.6446 =JCM 13530 =MCCC 1A00089).


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Neighbour-joining trees showing the phylogenetic positions of strain C10-2 and representatives of some other related taxa, based on 16S rRNA, , and gene sequences. [PDF](42 KB)


Transmission electron micrographs of three different negatively stained cells of strain C10-2 grown on LB agar for 24 h. Bar, 1 µm.


[PDF file of Supplementary Tables S1 and S2](48 KB)

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