1887

Abstract

A new actinobacterial strain, RS16, was isolated from sandy soil collected in Zamora, Spain, and was studied to determine its taxonomic position. A neighbour-joining tree based on 16S rRNA gene sequence analysis revealed that the novel isolate formed an independent branch between DSM 44121 and DSM 43110. Levels of 16S rRNA gene sequence similarity between the novel isolate and its phylogenetic neighbours ranged from 98.7 to 98.9 %. Chemotaxonomic properties, such as the predominant menaquinone and polar lipids, supported the assignment of the novel isolate to the genus , however, a significant number of physiological differences were found between the novel isolate and the other recognized species of the genus . On the basis of these results, it is proposed that strain RS16 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RS16 (=DSM 19349=LMG 24382).

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2008-06-01
2020-01-27
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References

  1. Busse, H.-J., Zlamala, C., Buczolits, S., Lubitz, W., Kämpfer, P. & Takeuchi, M. ( 2003; ). Promicromonospora vindobonensis sp. nov. and Promicromonospora aerolata sp. nov., isolated from the air in the medieval ‘Virgilkapelle’ in Vienna. Int J Syst Evol Microbiol 53, 1503–1507.[CrossRef]
    [Google Scholar]
  2. Cazemier, A. E., Verdoes, J. C., Reubsaet, F. A. G., Hackstein, J. H. P., van der Drift, C. & Op den Camp, H. J. M. ( 2003; ). Promicromonospora pachnodae sp. nov., a member of the (hemi) cellulolytic hindgut flora of larvae of the scarab beetle Pachnoda marginata. Antonie van Leeuwenhoek 83, 135–148.[CrossRef]
    [Google Scholar]
  3. Chun, J. ( 2001; ). phydit version 3.1 (available at http://plaza.snu.ac.kr/∼jchun/phydit/).
  4. Doetsch, R. N. ( 1981; ). Determinative methods of light microscopy. In Manual of Methods for General Bacteriology, pp. 21–33. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. H. Phillips. Washington, DC: American Society for Microbiology.
  5. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  6. Jones, K. L. ( 1949; ). Fresh isolates of actinomycetes in which the presence of sporogeneous aerial mycelia is a fluctuating characteristic. J Bacteriol 57, 141–145.
    [Google Scholar]
  7. Kalakoutskii, L. V., Agre, N. S., Prauser, H. & Evtushenko, L. I. ( 1989; ). Genus Promicromonospora Krasil'nikov, Kalakoutskii and Kirillova 1961a, 107AL. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2392–2395. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  8. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  9. Krasilnikov, N. A., Kalakoutskii, L. V. & Kirillova, N. F. ( 1961; ). A new genus of Actinomycetales, Promicromonospora gen. nov. Bull Acad Sci USSR Ser Biol 1, 107–112.
    [Google Scholar]
  10. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  11. MacKenzie, S. L. ( 1987; ). Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70, 151–160.
    [Google Scholar]
  12. Mandel, M. & Marmur, J. ( 1968; ). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B, 195–206.
    [Google Scholar]
  13. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  14. Rivas, R., Sánchez, M., Trujillo, M. E., Zurdo-Piñeiro, J. L., Mateos, P. F., Martínez-Molina, E. & Velázquez, E. ( 2003; ). Xylanimonas cellulosilytica gen. nov., sp. nov., a xylanolytic bacterium isolated from a decayed tree (Ulmus nigra). Int J Syst Evol Microbiol 53, 99–103.[CrossRef]
    [Google Scholar]
  15. Rivas, R., Trujillo, M. E., Schumann, P., Kroppenstedt, R. M., Sánchez, M., Mateos, P. F., Martínez-Molina, E. & Velázquez, E. ( 2004; ). Xylanibacterium ulmi gen. nov., sp. nov., a novel xylanolytic member of the family Promicromonosporaceae. Int J Syst Evol Microbiol 54, 557–561.[CrossRef]
    [Google Scholar]
  16. Schleifer, K. H. ( 1985; ). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123–156.
    [Google Scholar]
  17. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  18. Schröder, K.-H., Naumann, L., Kroppenstedt, R. M. & Reischl, U. ( 1997; ). Mycobacterium hassiacum sp. nov., a new rapidly growing thermophilic Mycobacterium. Int J Syst Bacteriol 47, 86–91.[CrossRef]
    [Google Scholar]
  19. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  20. Stackebrandt, E. & Ebers, J. ( 2006; ). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33, 152–155.
    [Google Scholar]
  21. Stackebrandt, E. & Schumann, P. ( 2004; ). Reclassification of Promicromonospora pachnodae Cazemier et al. 2004 as Xylanimicrobium pachnodae gen. nov., comb. nov. Int J Syst Evol Microbiol 54, 1383–1386.[CrossRef]
    [Google Scholar]
  22. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to the identification of aerobic actinomycetes by thin layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  23. Takahashi, Y., Tanaka, Y., Iwai, Y. & Omura, S. ( 1987; ). Promicromonospora sukumoe sp. nov., a new species of the Actinomycetales. J Gen Appl Microbiol 33, 507–519.[CrossRef]
    [Google Scholar]
  24. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  25. Trujillo, M. E., Fernández-Molinero, C., Velázquez, E., Kroppenstedt, R. M., Schumann, P., Mateos, P. F. & Martínez-Molina, E. ( 2005; ). Micromonospora mirobrigensis sp. nov. Int J Syst Evol Microbiol 55, 877–880.[CrossRef]
    [Google Scholar]
  26. Trujillo, M. E., Kroppenstedt, R. M., Schumann, P. & Martínez-Molina, E. ( 2006; ). Kribbella lupini sp. nov., isolated from the roots of Lupinus angustifolius. Int J Syst Evol Microbiol 56, 407–411.[CrossRef]
    [Google Scholar]
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Scanning electron micrographs of cells of strain RS16 after 28 h (A) and 72 h (B). [ PDF] 300 KB

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Fatty acid content of strain RS16 and related strains. [ PDF] 29 KB

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