1887

Abstract

A Gram-positive, spore-forming, halophilic bacterial strain, FP5, was isolated from a salt lake in southern Spain and subjected to a polyphasic taxonomic study. Strain FP5 was strictly aerobic. Cells were coccoidal, occurring singly or in clusters. The cell-wall peptidoglycan type of strain FP5 was A4 based on -Orn–-Asp. Strain FP5 was characterized chemotaxonomically by having MK-7 as the major menaquinone and anteiso-C, anteiso-C, iso-C and iso-C as the main fatty acids. The isolate grew optimally at 37 °C and in presence of 10 % NaCl; no growth was observed in the absence of NaCl. The DNA G+C content was 43.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain FP5 falls within the evolutionary radiation of species of the genus . Levels of 16S rRNA gene sequence similarity between strain FP5 and the type strains of nine recognized species were in the range 97.0–99.0 %. Levels of DNA–DNA relatedness indicated that strain FP5 represents a genomic species that is distinct from recognized species. Strain FP5 could be differentiated from recognized species based on several phenotypic characteristics. On the basis of phenotypic, phylogenetic and genomic data, strain FP5 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FP5 (=DSM 18525 =ATCC BAA-1361).

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2008-04-01
2019-10-19
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Scanning electronic micrograph of cells of strain FP5 . Bar, 5 µm.

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Total polar lipids of strain FP5 . (A) Two-dimensional TLC of phospholipids detected by spraying the plate with Dittmer and Lester reagent. (B) Two-dimensional TLC of glycolipids detected by spraying the plate with α-naphthol reagent. The solvent system was chloroform/methanol/water (65:25:4 by vol.) for the first dimension and chloroform/methanol/acetic acid/water (85:15:12:4 by vol.) for the second dimension. (C) TLC of total polar lipids; the right half was sprayed with α-naphthol reagent and the left half was sprayed with Dittmer and Lester reagent. The solvent system was chloroform/methanol/water (65:25:4 by vol.).

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