We have used amplified fragment length polymorphism (AFLP), multilocus sequence analysis (MLSA) and DNA–DNA hybridization for genotypic classification of pathovars associated with the plant family Anacardiaceae. AFLP and MLSA results showed congruent phylogenetic relationships of the pathovar (responsible for mango bacterial canker) with strains of subgroup 9.5. This subgroup includes pv. (synonym ). Similarly, the pathovar , which causes cashew bacterial spot in Brazil, was included in subgroup 9.6 (synonym ). Based on the thermal stability of DNA reassociation, consistent with the AFLP and MLSA data, the two pathovars share a level of similarity consistent with their being members of the same species. The recent proposal to elevate pv. to species level as is supported by our data. Therefore, the causal agents of mango bacterial canker and cashew bacterial spot should be classified as pathovars of , namely pv. (pathotype strain CFBP 1716) and pv. (pathotype strain CFBP 2913), respectively. should be considered to be a later heterotypic synonym of .


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