1887

Abstract

While studying the taxonomy of six lactic acid bacterium isolates from Finnish porcine intestine and faeces, the taxonomic positions of type strain DSM 16698 and strain AD5 based on comparative 16S rRNA sequence analysis were found to be controversial, as they showed high similarity to strains. Therefore, the taxonomy of these species was addressed in a polyphasic taxonomy study that included, in addition to re-evaluating the 16S rRNA gene sequence and DNA–DNA reassociation results, multilocus sequence analysis (MLSA) of the housekeeping genes encoding the phenylalanyl-tRNA synthase alpha subunit () and RNA polymerase alpha subunit () as well as numerical analysis of dIII and RI ribotypes. 16S rRNA gene sequence analysis demonstrated a very high similarity between the and type and reference strains and representative Finnish porcine isolates (99.6–99.9 %). The MLSA data showed the close phylogenetic relationship of these strains; and gene sequence similarities were 98.5–100 % and 99.6–99.8 %, respectively. Numerical analyses of dIII/RI ribotypes placed these strains in a single cluster by both enzymes. Finally, the DNA–DNA reassociation experiments revealed high reassociation levels (higher than 79 %) between the strains. These results indicate that DSM 16698, AD5 and the related porcine lactobacilli strains from Finland constitute a single species, , and that the name should be considered as a later synonym of .

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2008-04-01
2019-10-21
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Phylogenetic trees trees obtained with 16S rRNA , and gene sequences of selected strains. [PDF](750 KB)

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DNA relatedness among strains and phylogenetically closely related species. [PDF](17 KB)

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