Four thermophilic, sulfur-oxidizing, chemolithoautotrophic strains with >99 % 16S rRNA gene sequence similarity were isolated from terrestrial hot springs in the Geyser Valley and the Uzon Caldera, Kamchatka, Russia. One strain, designated UZ3-5, was characterized fully. Cells of UZ3-5 were Gram-negative, motile, slightly oval rods (about 0.7 μm wide and 1.0 μm long) with multiple polar flagella. All four strains were obligately microaerophilic chemolithoautotrophs and could use elemental sulfur or thiosulfate as electron donors and oxygen (1–14 %, v/v) as the electron acceptor. Strain UZ3-5 grew at temperatures between 55 and 80 °C (optimally at 75 °C; 1.1 h doubling time), at pH 5.0–7.2 (optimally at pH 6.0–6.3) and at 0–0.9 % NaCl (optimally in the absence of NaCl). The G+C content of the genomic DNA of strain UZ3-5 was 35 mol%. Phylogenetic analysis revealed that strain UZ3-5 was a member of the genus , its closest relative in culture being Az-Fu1 (98.3 % 16S rRNA gene sequence similarity). On the basis of its physiological and molecular characteristics, strain UZ3-5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is UZ3-5 (=OCM 900 =ATCC BAA-1536 =DSM 19533).


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Effects of temperature (S1; left) and pH (S2; right) on growth of strain UZ3-5 .


Effects of temperature (S1; left) and pH (S2; right) on growth of strain UZ3-5 .


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