1887

Abstract

A novel aerobic, Gram-negative, rod-shaped, motile bacterium, designated strain 5516S-11, was isolated from air samples collected in the Suwon region of the Republic of Korea. Analysis of the 16S rRNA gene sequence indicated that the organism belongs to the genus ; the highest sequence similarity (97.2 %) was found with respect to DSM 18055. Cells of strain 5516S-11 contained ubiquinone Q-8 as the predominant isoprenoid quinone and possessed summed feature 3 (C 7/iso-C 2-OH; 35.2 %), C (30.6 %) and C 7 (11.7 %) as the major fatty acids. DNA–DNA hybridization revealed 32 % relatedness between strain 5516S-11 and DSM 18055. The G+C content of the DNA of strain 5516S-11 was 68.9 mol%. It is clear from the genotypic and phenotypic data presented that strain 5516S-11 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 5516S-11 (=KACC 12505 =DSM 19289).

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2008-06-01
2020-01-21
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References

  1. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  2. Gallego, V., Sánchez-Porro, C., García, M. T. & Ventosa, A. ( 2006; ). Massilia aurea sp. nov., isolated from drinking water. Int J Syst Evol Microbiol 56, 2449–2453.[CrossRef]
    [Google Scholar]
  3. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  4. Hiraishi, A. ( 1992; ). Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett Appl Microbiol 15, 210–213.[CrossRef]
    [Google Scholar]
  5. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  6. La Scola, B., Birtles, R. J., Mallet, M. N. & Raoult, D. ( 1998; ). Massilia timonae gen. nov., sp. nov., isolated from blood of an immunocompromised patient with cerebellar lesions. J Clin Microbiol 36, 2847–2852.
    [Google Scholar]
  7. Lindquist, D., Murrill, D., Burran, W. P., Winans, G., Janda, J. M. & Probert, W. ( 2003; ). Characteristics of Massilia timonae and Massilia timonae-like isolates from human patients, with an emended description of the species. J Clin Microbiol 41, 192–196.[CrossRef]
    [Google Scholar]
  8. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  9. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  10. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  11. Seldin, L. & Dubnau, D. ( 1985; ). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35, 151–154.[CrossRef]
    [Google Scholar]
  12. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  13. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  14. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  15. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J. ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703.
    [Google Scholar]
  16. Zhang, Y.-Q., Li, W.-J., Zhang, K.-Y., Tian, X.-P., Jiang, Y., Xu, L.-H., Jiang, C.-L. & Lai, R. ( 2006; ). Massilia dura sp. nov., Massilia albidiflava sp. nov., Massilia plicata sp. nov. and Massilia lutea sp. nov., isolated from soils in China. Int J Syst Evol Microbiol 56, 459–463.[CrossRef]
    [Google Scholar]
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