1887

Abstract

A Gram-negative, rod-shaped bacterium, designated strain EMB320, was isolated from activated sludge performing enhanced biological phosphorus removal in a sequencing batch reactor. The isolate was strictly aerobic and non-motile. Growth was observed between 10 and 35 °C (optimum 30 °C) and between pH 6.0 and 9.0 (optimum pH 7.0–8.0). The predominant cellular fatty acids of strain EMB320 were C, C 7 and summed feature 3 (C 7 and/or iso-C 2-OH). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Strain EMB320 contained ubiquinone-8 (Q-8) as the major respiratory quinone system and 2-hydroxyputrescine and putrescine as the major polyamines, which suggests that it belongs to the . The G+C content of the genomic DNA was 62.7 mol%. Comparative 16S rRNA gene sequence analysis showed that strain EMB320 formed a phyletic lineage distinct from other genera within the family . On the basis of chemotaxonomic data and molecular properties, strain EMB320 represents a novel genus and species within the family , for which the name sp. nov. is proposed. The type strain of is EMB320 (=KCTC 12616 =DSM 17982).

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2008-05-01
2019-09-16
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Transmission electron micrograph showing the general morphology of negatively stained cells of strain EMB320 after growth for 5 days at 30 °C on R2A broth. Bar, 1 µm.

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Polar lipid TLC sprayed with molybdatophosphoric acid reagent of strain EMB320 . Ascending solvent system: (I) chloroform/methanol/water (65:25:4, by vol.); (II) chloroform/acetic acid/methanol/water (80:13:10:2, by vol.). For detection of polar lipids, molybdatophosphoric acid (PE, PG, DPG and AL), ninhydrin (PE and AL) and molybdenum blue reagent (PG, PE and DPG) were applied. The unknown was inferred as an amino group-containing lipid. PE, Phosphatidylethanolamine; PG, phosphatidylglycerol; DPG, diphosphatidylglycerol; AL, amino group-containing lipid.

IMAGE

Maximum-likelihood (a) and maximum-parsimony (b) trees showing phylogenetic relationships of strain EMB320 and related taxa. ATCC 25416 was used as an outgroup. Bars, 0.1 changes per nucleotide position. [PDF](21 KB)

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