Four Gram-negative, rod-shaped, non-spore-forming, denitrifying isolates were obtained from the activated sludge of an aerobic–anaerobic wastewater treatment plant in Belgium. Analysis of repetitive sequence-based PCR showed that the four isolates were genetically different from each other. Results of 16S rRNA gene sequence analysis and DNA–DNA hybridization experiments indicated that these four isolates were affiliated to the genus and could be differentiated from all recognized species of the genus. Analysis of whole-cell proteins and results of physiological and biochemical tests allowed differentiation of the new isolates from their closest phylogenetic neighbours. These new isolates therefore represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is R-24608 (=LMG 24103 =DSM 19327).


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vol. , part 1, pp. 73 - 77

Grouping of normalized digitized SDS-PAGE patterns. [PDF](213 KB)


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