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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 58, Issue 4

16S rRNA-, GroEL- and MucZ-based assessment of the taxonomic position of ‘’ and its implications for the organization of the genus Free

Abstract

‘’ is an intracellularly multiplying bacterial pathogen of European cockchafers, (Linnaeus, 1758) and (Fabricius, 1801) (Coleoptera: Scarabaeidae). We report the first determination of nucleotide sequences from this organism, i.e. the 16S rRNA encoding gene, the chaperonin encoding gene and the gene encoding the orthologue of a capsule synthesis-inducing factor of . Within the genus , the pathotype ‘’ is currently classified as a synonym of the nomenclatural type species . Previous sequencing of a 16S rRNA gene from a different species, has motivated the transfer of the entire genus from the alphaproteobacterial order to the gammaproteobacterial order , family . We investigated the validity of this taxonomic reorganization beyond the species by reconstructing the organismal phylogeny from comparisons of 16S rRNA gene and GroEL and MucZ protein sequences from a selected set of alpha- and gammaproteobacteria as well as bacterial pathogens from the order . Our analysis strongly supported the transfer of the genus to the order , but not its classification in one of the recognized families present in this order. Furthermore, our results substantiated inconsistencies in the internal organization of the genus. In particular, the currently accepted delineation of species and the claimed synonymy of ‘’ with are not simultaneously consistent with our findings.

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Keyword(s): ME, minimum evolution , ML, maximum-likelihood , MP, maximum-parsimony and NJ, neighbour-joining
© SGM
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2008-04-01
2024-05-03
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16S rRNA-, GroEL- and MucZ-based assessment of the taxonomic position of ‘Rickettsiella melolonthae’ and its implications for the organization of the genus Rickettsiella
Int J Syst Evol Microbiol 58, 749 (2008); https://doi.org/10.1099/ijs.0.65359-0
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