A Gram-positive, motile, moderately halophilic and rod-shaped bacterium, strain AHS-1, was isolated from a marine solar saltern in Korea, and its exact taxonomic position was investigated by use of a polyphasic study. Strain AHS-1 grew optimally at pH 6.5–7.0 and 37–40 °C in the presence of 10–12 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain AHS-1 belonged to the genus . Strain AHS-1 exhibited a 16S rRNA gene sequence similarity value of 97.0 % to the type strain of and values of 95.0–96.7 % to the type strains of other recognized species. Strain AHS-1 had cell-wall peptidoglycan based on -diaminopimelic acid. It contained MK-7 as the predominant menaquinone. The major fatty acids (>10 % of total fatty acids) were anteiso-C and anteiso-C, and the major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified glycolipids. The DNA G+C content was 49.0 mol%. Strain AHS-1 shared a mean level of DNA–DNA relatedness of 8.5 % with . KCTC 3911. Strain AHS-1 could also be distinguished from recognized species based on several phenotypic properties. On the basis of phenotypic, chemotaxonomic, phylogenetic and genetic data, strain AHS-1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AHS-1 (=KCTC 13162=CCUG 54822).


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vol. , part 1, pp. 45 - 49

Cellular fatty acid compositions of sp. nov. strain AHS-1 and other recognized species. [PDF](77 KB)

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