1887

Abstract

Three alkaliphilic and moderately halophilic strains designated K1-5, K1-10 and B1-1, characterized by optimal growth at pH 9.0–10.0 and at 3–7 % (w/v) NaCl, were isolated from extremely shallow, alkaline soda lakes located in Hungary. Cells of the strains are Gram-positive, straight rods and form a central to subterminal, ellipsoidal endospore. The isolates are strictly aerobic, catalase-positive, oxidase-negative and contain a peptidoglycan of type A1 based on -diaminopimelic acid. In strain K1-5, menaquinone-7 (MK-7) is the predominant isoprenoid quinone and anteiso-C is the major cellular fatty acid. The DNA G+C content of strain K1-5 is 42.9 mol%. 16S rRNA gene-based phylogenetic analysis revealed that the strains exhibit levels of sequence similarity of less than 95.8 % to known species. According to the polyphasic characterization, the strains represent a novel species, for which the name sp. nov. is proposed. The type strain is K1-5 (=DSM 18675 =CCM 7447 =NCAIM B002265).

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2008-04-01
2019-10-15
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