1887

Abstract

Two coloured bacteria were isolated from the same plate for detecting cultivable bacteria from the air of Xinjiang in China. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the isolates were members of the genus , in which they represented two novel lineages. Although the two strains presented high 16S rRNA gene sequence similarity (above 97 %), their DNA G+C contents were very different (6 mol%). The G+C contents of strains HO-9041 and HO-9042 are 71 and 65 mol%, respectively. DNA relatedness analysis and other taxonomic evidence supports the placement of the two isolates in the genus . The diagnostic diamino acid of the cell-wall peptidoglycan is -lysine and both strains contain MK-8(H) and MK-9(H) as major menaquinones. In addition, they share similar fatty acid patterns containing straight-chain saturated and iso- and anteiso-branched acids, with a major component being anteiso-C. Genotypic, morphological and physiological characteristics are used to describe two novel species of , for which the names sp. nov. (type strain HO-9041 =CCTCC AB 206106 =KCTC 19306) and sp. nov. (type strain HO-9042 =CCTCC AB 206107 =KCTC 19307) are proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65323-0
2008-06-01
2019-10-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/6/1304.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65323-0&mimeType=html&fmt=ahah

References

  1. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  2. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  3. Kim, S. B., Nedashkovskaya, O. I., Mikhailov, V. V., Han, S. K., Kim, K.-O., Rhee, M. S. & Bae, K. S. ( 2004; ). Kocuria marina sp. nov., a novel actinobacterium isolated from marine sediment. Int J Syst Evol Microbiol 54, 1617–1620.[CrossRef]
    [Google Scholar]
  4. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  5. Kovács, G., Burghardt, J., Pradella, S., Schumann, P., Stackebrandt, E. & Màrialigetti, K. ( 1999; ). Kocuria palustris sp. nov. and Kocuria rhizophila sp. nov., isolated from the rhizoplane of the narrow-leaved cattail (Typha angustifolia). Int J Syst Bacteriol 49, 167–173.[CrossRef]
    [Google Scholar]
  6. Kumagai, M., Fujimoto, M. & Kuninaka, A. ( 1988; ). Determination of base composition of DNA by high performance liquid chromatography of its nuclease P1 hydrolysate. Nucleic Acids Symp Ser 19, 65–68.
    [Google Scholar]
  7. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  8. Li, W.-J., Zhang, Y.-Q., Schumann, P., Chen, H.-H., Hozzein, W. N., Tian, X.-P., Xu, L.-H. & Jiang, C.-L. ( 2006; ). Kocuria aegyptia sp. nov., a novel actinobacterium isolated from a saline, alkaline desert soil in Egypt. Int J Syst Evol Microbiol 56, 733–737.[CrossRef]
    [Google Scholar]
  9. Mayilraj, S., Kroppenstedt, R. M., Suresh, K. & Saini, H. S. ( 2006; ). Kocuria himachalensis sp. nov., an actinobacterium isolated from the Indian Himalayas. Int J Syst Evol Microbiol 56, 1971–1975.[CrossRef]
    [Google Scholar]
  10. Murray, R. G. E., Doetsch, R. N. & Robinow, F. ( 1994; ). Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  11. Pandey, K. K., Mayilraj, S. & Chakrabarti, T. ( 2002; ). Pseudomonas indica sp. nov., a novel butane-utilizing species. Int J Syst Evol Microbiol 52, 1559–1567.[CrossRef]
    [Google Scholar]
  12. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E. ( 1996; ). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsiaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef]
    [Google Scholar]
  13. Rainey, F. A., Nobre, M. F., Schumann, P., Stackebrandt, E. & Da Costa, M. S. ( 1997; ). Phylogenetic diversity of the deinococci as determined by 16S ribosomal DNA sequence comparison. Int J Syst Bacteriol 47, 510–514.[CrossRef]
    [Google Scholar]
  14. Reddy, G. S. N., Prakash, J. S. S., Prabahar, V., Matsumoto, G. I., Stackebrandt, E. & Shivaji, S. ( 2003; ). Kocuria polaris sp. nov., an orange-pigmented psychrophilic bacterium isolated from an Antarctic cyanobacterial mat sample. Int J Syst Evol Microbiol 53, 183–187.[CrossRef]
    [Google Scholar]
  15. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  16. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  17. Schumann, P., Spröer, C., Burghardt, J., Kovacs, G. & Stackebrandt, E. ( 1999; ). Reclassification of the species Kocuria erythromyxa (Brooks and Murray 1981) as Kocuria rosea (Flügge 1886). Int J Syst Bacteriol 49, 393–396.[CrossRef]
    [Google Scholar]
  18. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  19. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  20. Stackebrandt, E., Koch, C., Gvozdiak, O. & Schumann, P. ( 1995; ). Taxonomic dissection of the genus Micrococcus: Kocuria gen. nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend. Int J Syst Bacteriol 45, 682–692.[CrossRef]
    [Google Scholar]
  21. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  22. Tvrzová, L., Schumann, P., Sedláček, I., Páčová, Z., Spröer, C., Verbarg, S. & Kroppenstedt, R. M. ( 2005; ). Reclassification of strain CCM 132, previously classified as Kocuria varians, as Kocuria carniphila sp. nov. Int J Syst Evol Microbiol 55, 139–142.[CrossRef]
    [Google Scholar]
  23. Wieser, M., Denner, E. B. M., Kämpfer, P., Schumann, P., Tindall, B., Steiner, U., Vybiral, D., Lubitz, W., Maszenan, A. M. & other authors ( 2002; ). Emended descriptions of the genus Micrococcus, Micrococcus luteus (Cohn 1872) and Micrococcus lylae (Kloos et al. 1974). Int J Syst Evol Microbiol 52, 629–637.
    [Google Scholar]
  24. Xie, C. H. & Yokota, A. ( 2003; ). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49, 345–349.[CrossRef]
    [Google Scholar]
  25. Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H. ( 1996; ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46, 502–505.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65323-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65323-0
Loading

Data & Media loading...

Supplements

vol. , part 6, pp. 1304 - 1307

Fatty acid compositions of strains HO-9041 and HO-9042 and the type strains of the most closely related species of the genus . [PDF](20 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error