1887

Abstract

A Gram-negative, yellow-pigmented bacterium capable of gliding motility, designated strain WPCB118, was isolated from fresh water collected from the Woopo wetland (Republic of Korea). Cells were rod-shaped and sometimes filamentous. The major fatty acids were iso-C 2-OH and/or C 7 (45.6 %), C 5 (18.5 %), iso-C (9.5 %) and C (8.8 %). The predominant menaquinone and polar lipid were MK-7 and phosphatidylethanolamine, respectively. The DNA G+C content was 53.3 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain WPCB118 had an evolutionary lineage within the radiation encompassing the members of the family ‘’, its closest neighbour being LMG 10896 (93.7 % gene sequence similarity). Data from this polyphasic study indicated that strain WPCB118 could not be assigned to any recognized species. Strain WPCB118 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WPCB118 (=KCTC 12531=NBRC 101117).

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2007-12-01
2024-04-27
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References

  1. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
    [Google Scholar]
  2. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [CrossRef]
    [Google Scholar]
  3. Chun J., Bae K. S., Moon E. Y., Jung S. O., Lee H. K., Kim S. J. 2000; Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol 50:1909–1913
    [Google Scholar]
  4. Embley T. M., Wait R. 1994; Structural lipids of eubacteria. In Chemical Methods in Prokaryotic Systematics pp 121–161 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  6. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees. Science 155:279–284 [CrossRef]
    [Google Scholar]
  7. Gosink J. J., Woese C. R., Staley J. T. 1998; Polaribacter gen. nov., with three new species, P.irgensii sp. nov., P. franzmannii sp.nov. and P. filamentus sp. nov.,gas vacuolated polar marine bacteria of the Cytophaga–Flavobacterium–Bacteroides group and reclassification of ‘ Flectobacillus glomeratus ’ as Polaribacter glomeratus comb. nov. Int J Syst Bacteriol 48:223–235 [CrossRef]
    [Google Scholar]
  8. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  9. Kovács N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178:703
    [Google Scholar]
  10. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics pp 173–199 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  11. Larkin J. M., Borrall R. 1984; Family I. Spirosomaceae Larkin and Borrall 1978, 595AL . In Bergey's Manual of Systematic Bacteriology vol 1 pp 125–126 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  12. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  13. Minnikin D. E., Alshamaony L., Goodfellow M. 1975; Differentiation of Mycobacterium , Nocardia , and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88:200–204 [CrossRef]
    [Google Scholar]
  14. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  15. NCCLS 2003 Performance standards for antimicrobial disk susceptibility tests , 8th edn.. Approved Standard, M2-A8 Wayne, PA: National Committee for Clinical Laboratory Standards;
    [Google Scholar]
  16. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  17. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium , new prosthecate. freshwater bacteria. J Bacteriol 95:1921–1944
    [Google Scholar]
  18. Swofford D. L. 1998 paup: Phylogenetic analysis using parsimony, version 4 Sunderland, MA: Sinauer Associates;
    [Google Scholar]
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