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An aerobic bacterial isolate, strain HN-182T, was isolated from sediments of the South China Sea. Cells of strain HN-182T are coccoid to short rods, Gram-negative, non-spore-forming and non-motile. Strain HN-182T is heterotrophic and grows well on marine broth (Difco 2216), and is not capable of growing autotrophically on reduced sulfur. Grows at temperatures ranging from 7 to 42 °C (optimum at 25 °C), but not at 4 or 45 °C, and at pH 5.0–9.0 (optimum at pH 7.0), but not at pH 4.5 or 9.5. NaCl is required for growth [0.5–8.5 % (w/v)] with an optimum of 4.5 %. Cells are positive for catalase, oxidase and urease activities. Nitrate is not reduced. Strain HN-182T contains ubiquinone-10 as sole respiratory quinone. The major polar lipids are phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unidentified phospholipid and an unidentified glycolipid. Major cellular fatty acids are C18 : 1ω7c (60.7 %), C16 : 0 (12.5 %) and C18 : 0 (8.1 %). DNA G+C content is 67.2 mol% (by T m). The analysis of 16S rRNA gene sequences indicated that strain HN-182T was related to members of the genus Paracoccus, with similarities ranging from 91.2 to 96.7 % (highest to Paracoccus versutus) and a close relationship with Paracoccus sulfuroxidans, indicating that strain HN-182T is a member of Paracoccus. Based on these results, it is concluded that strain HN-182T represents a novel species of the genus Paracoccus, for which the name Paracoccus halophilus sp. nov. is proposed. The type strain is HN-182T (=CGMCC 1.6117T=JCM 14014T).
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International Journal of Systematic and Evolutionary Microbiology vol. 58 , part 1, pp.257–261
Supplementary Fig. S1. Morphology of strain HN-182 Tgrown at 30 °C on marine broth 2216 (Difco) for 1 day.
Supplementary Fig. S2. Polar lipids examined by two-dimensional TLC.
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