1887

International Journal of Systematic and Evolutionary Microbiology

Volume 58, Issue 1

sp. nov., isolated from marine sediment of the South China Sea, China, and emended description of genus Davis 1969 Free

Abstract

An aerobic bacterial isolate, strain HN-182, was isolated from sediments of the South China Sea. Cells of strain HN-182 are coccoid to short rods, Gram-negative, non-spore-forming and non-motile. Strain HN-182 is heterotrophic and grows well on marine broth (Difco 2216), and is not capable of growing autotrophically on reduced sulfur. Grows at temperatures ranging from 7 to 42 °C (optimum at 25 °C), but not at 4 or 45 °C, and at pH 5.0–9.0 (optimum at pH 7.0), but not at pH 4.5 or 9.5. NaCl is required for growth [0.5–8.5 % (w/v)] with an optimum of 4.5 %. Cells are positive for catalase, oxidase and urease activities. Nitrate is not reduced. Strain HN-182 contains ubiquinone-10 as sole respiratory quinone. The major polar lipids are phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unidentified phospholipid and an unidentified glycolipid. Major cellular fatty acids are C18 : 17 (60.7 %), C16 : 0 (12.5 %) and C18 : 0 (8.1 %). DNA G+C content is 67.2 mol% (by ). The analysis of 16S rRNA gene sequences indicated that strain HN-182 was related to members of the genus , with similarities ranging from 91.2 to 96.7 % (highest to ) and a close relationship with , indicating that strain HN-182 is a member of . Based on these results, it is concluded that strain HN-182 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HN-182 (=CGMCC 1.6117=JCM 14014).

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65237-0
2008-01-01
2020-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/1/257.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65237-0&mimeType=html&fmt=ahah

References

  1. Allen, M. B. ( 1959; ). Studies with Cyanidium caldarium, an anomalously pigmented chlorophyte. Arch Mikrobiol 32, 270–277.[CrossRef]
    [Google Scholar]
  2. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef]
    [Google Scholar]
  3. Berry, A., Janssens, D., Hümbelin, M., Jore, J. P. M., Hoste, B., Cleenwerck, I., Vancanneyt, M., Bretzel, W., Mayer, A. F. & other authors ( 2003; ). Paracoccus zeaxanthinifaciens sp. nov., a zeaxanthin-producing bacterium. Int J Syst Evol Microbiol 53, 231–238.[CrossRef]
    [Google Scholar]
  4. Collins, M. D. ( 1985; ). Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  5. Davis, D. H., Doudoroff, M., Stanier, R. Y. & Mandel, M. ( 1969; ). Proposal to reject the genus Hydrogenomonas: taxonomic implications. Int J Syst Bacteriol 19, 375–390.[CrossRef]
    [Google Scholar]
  6. Denner, E. B. M., Kolari, M., Hoornstra, D., Tsitko, I., Kämpfer, P., Busse, H.-J. & Salkinoja-Salonen, M. ( 2006; ). Rubellimicrobium thermophilum gen. nov., sp. nov., a red-pigmented, moderately thermophilic bacterium isolated from coloured slime deposits in paper machines. Int J Syst Evol Microbiol 56, 1355–1362.[CrossRef]
    [Google Scholar]
  7. Dong, X.-Z. & Cai, M.-Y. ( 2001; ). Determinative Manual for Routine Bacteriology. Beijing: Scientific Press (English translation).
  8. Doronina, N. V., Trotsenko, Y. A., Krausova, V. I. & Suzina, N. E. ( 1998; ). Paracoccus methylutens sp. nov. – a new aerobic facultatively methylotrophic bacterium utilizing dichloromethane. Syst Appl Microbiol 21, 230–236.[CrossRef]
    [Google Scholar]
  9. Doronina, N. V., Trotsenko, Y. A., Kuznetzov, B. B. & Tourova, T. P. ( 2002; ). Emended description of Paracoccus kondratievae. Int J Syst Evol Microbiol 52, 679–682.
     [Google Scholar]
  10. Fujii, K., Satomi, M., Morita, N., Motomura, T., Tanaka, T. & Kikuchi, S. ( 2003; ). Novosphingobium tardaugens sp. nov., an oestradiol-degrading bacterium isolated from activated sludge of a sewage treatment plant in Tokyo. Int J Syst Evol Microbiol 53, 47–52.[CrossRef]
    [Google Scholar]
  11. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors) ( 1994; ). Methods for general and molecular bacteriology. Washington, D. C.: American Society for Microbiology.
  12. Ghosh, W., Mandal, S. & Roy, P. ( 2006; ). Paracoccus bengalensis sp. nov., a novel sulfur-oxidizing chemolithoautotroph from the rhizosphere soil of an Indian tropical leguminous plant. Syst Appl Microbiol 29, 396–403.[CrossRef]
    [Google Scholar]
  13. Hu, Y.-T., Zhou, P.-J., Zhou, Y.-G., Liu, Z.-H. & Liu, S.-J. ( 2004; ). Saccharothrix xingjiangensis, sp. nov., a pyrene-degrading actinomycete isolated from Tianchi lake, Xinjiang, China. Int J Syst Evol Microbiol 54, 2091–2094.[CrossRef]
    [Google Scholar]
  14. Hugh, R. & Leifson, E. ( 1953; ). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram-negative bacteria. J Bacteriol 66, 24–26.
     [Google Scholar]
  15. Katayama, Y., Hiraishi, A. & Kuraishi, H. ( 1995; ). Paracoccus thiocyanatus sp. nov., a new species of thiocyanate-utilizing facultative chemolithotroph, and transfer of Thiobacillus versutus to the genus Paracoccus as Paracoccus versutus comb. nov. with emendation of the genus. Microbiology 141, 1469–1477.[CrossRef]
    [Google Scholar]
  16. Kelly, D. P., Euzéby, J. P., Goodhew, C. F. & Wood, A. P. ( 2006a; ). Redefining Paracoccus denitrificans and Paracoccus pantotrophus and the case for a reassessment of the strains held by international culture collections. Int J Syst Evol Microbiol 56, 2495–2500.[CrossRef]
    [Google Scholar]
  17. Kelly, D. P., Rainey, F. A. & Wood, A. P. ( 2006b; ). The genus Paracoccus. In The Prokaryotes, A Handbook on the Biology of Bacteria, 3rd edn, vol. 5, pp. 232–249. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer.
  18. Kim, B.-Y., Weon, H.-Y., Yoo, S.-H., Kwon, S.-W., Cho, Y.-H., Stackebrandt, E. & Go, S.-J. ( 2006; ). Paracoccus homiensis sp. nov., isolated from a sea-sand sample. Int J Syst Evol Microbiol 56, 2387–2390.[CrossRef]
    [Google Scholar]
  19. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  20. La, H. J., Im, W.-T., Ten, L. N., Kang, M. S., Shin, D. Y. & Lee, S. T. ( 2005; ). Paracoccus koreensis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket (UASB) reactor. Int J Syst Evol Microbiol 55, 1657–1660.[CrossRef]
    [Google Scholar]
  21. Labrenz, M., Collins, M. D., Lawson, P. A., Tindall, B. J., Braker, G. & Hirsch, P. ( 1998; ). Antarctobacter heliothermus gen. nov., sp. nov., a budding bacterium from hypersaline and heliothermal Ekho Lake. Int J Syst Bacteriol 48, 1363–1372.[CrossRef]
    [Google Scholar]
  22. Labrenz, M., Collins, M. D., Lawson, P. A., Tindall, B. J., Schumann, P. & Hirsch, P. ( 1999; ). Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. Int J Syst Bacteriol 49, 137–147.[CrossRef]
    [Google Scholar]
  23. Labrenz, M., Lawson, P. A., Tindall, B. J., Collins, M. D. & Hirsch, P. ( 2005; ). Roseisalinus antarcticus gen. nov., sp. nov., a novel aerobic bacteriochlorophyll a-producing α-proteobacterium isolated from hypersaline Ekho Lake, Antarctica. Int J Syst Evol Microbiol 55, 41–47.[CrossRef]
    [Google Scholar]
  24. Lee, J. H., Kim, Y. S., Choi, T.-J., Lee, W. J. & Kim, Y. T. ( 2004; ). Paracoccus haeundaensis sp. nov., a Gram-negative, halophilic, astaxanthin-producing bacterium. Int J Syst Evol Microbiol 54, 1699–1702.[CrossRef]
    [Google Scholar]
  25. Lipski, A., Reichert, K., Reuter, B., Sproer, C. & Altendorf, K. ( 1998; ). Identification of bacterial isolates from biofilters as Paracoccus alkenifer sp. nov. and Paracoccus solventivorans with emended description of Paracoccus solventivorans. Int J Syst Bacteriol 48, 529–536.[CrossRef]
    [Google Scholar]
  26. Liu, X.-Y., Wang, B.-J., Jiang, C.-Y. & Liu, S.-J. ( 2006; ). Paracoccus sulfuroxidans sp. nov., a sulfur oxidizer from activated sludge. Int J Syst Evol Microbiol 56, 2693–2695.[CrossRef]
    [Google Scholar]
  27. Ludwig, W., Mittenhuber, G. & Friedrich, C. G. ( 1993; ). Transfer of Thiosphaera pantotropha to Paracoccus denitrificans. Int J Syst Bacteriol 43, 363–367.[CrossRef]
    [Google Scholar]
  28. Macián, M. C., Arahal, D. R., Garay, E., Ludwig, W., Schleifer, K. H. & Pujalte, M. J. ( 2005; ). Thalassobacter stenotrophicus gen. nov., sp. nov., a novel marine α-proteobacterium isolated from Mediterranean sea water. Int J Syst Evol Microbiol 55, 105–110.[CrossRef]
    [Google Scholar]
  29. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  30. Pukall, R., Laroche, M., Kroppenstedt, R. M., Schumann, P., Stackebrandt, E. & Ulber, R. ( 2003; ). Paracoccus seriniphilus sp. nov., an l-serine-dehydratase-producing coccus isolated from the marine bryozoan Bugula plumosa. Int J Syst Evol Microbiol 53, 443–447.[CrossRef]
    [Google Scholar]
  31. Rainey, F. A., Kelly, D. P., Stackebrandt, E., Burghardt, J., Hiraishi, A., Katayama, Y. & Wood, A. P. ( 1999; ). A re-evaluation of the taxonomy of Paracoccus denitrificans and a proposal for the combination Paracoccus pantotrophus comb. nov. Int J Syst Bacteriol 49, 645–651.[CrossRef]
    [Google Scholar]
  32. Robertson, L. A. & Kuenen, J. G. ( 1983; ). Thiosphaera pantotropha gen. nov. sp. nov., a facultatively anaerobic, facultatively, autotrophic sulphur bacterium. J Gen Microbiol 129, 2847–2855.
     [Google Scholar]
  33. Siller, H., Rainey, F. A., Stackebrandt, E. & Winter, J. ( 1996; ). Isolation and characterization of a new Gram-negative, acetone-degrading, nitrate-reducing bacterium from soil, Paracoccus solventivorans sp. nov. Int J Syst Bacteriol 46, 1125–1130.[CrossRef]
    [Google Scholar]
  34. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  35. Tsubokura, A., Yoneda, H. & Mizuta, H. ( 1999; ). Paracoccus carotinifaciens sp. nov., a new aerobic Gram-negative astaxanthin-producing bacterium. Int J Syst Bacteriol 49, 277–282.[CrossRef]
    [Google Scholar]
  36. Ventosa, A., Marquez, M. C., Kocur, M. & Tindall, B. J. ( 1993; ). Comparative study of “Micrococcus sp.” strains CCM 168 and CCM 1405 and members of the genus Salinicoccus. Int J Syst Bacteriol 43, 245–248.[CrossRef]
    [Google Scholar]
  37. Wu, C., Lu, X., Qin, M., Wang, Y. & Ruan, J. ( 1989; ). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology English translation of Microbiology (Beijing) 16, 176–178.
     [Google Scholar]
  38. Zhang, D., Yang, H., Zhang, W., Huang, Z. & Liu, S.-J. ( 2003; ). Rhodocista pekingensis sp. nov., a cyst-forming phototrophic bacterium from a municipal wastewater treatment plant. Int J Syst Evol Microbiol 53, 1111–1114.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65237-0
Loading
Paracoccus halophilus sp. nov., isolated from marine sediment of the South China Sea, China, and emended description of genus Paracoccus Davis 1969
Int J Syst Evol Microbiol 58, 257 (2008); https://doi.org/10.1099/ijs.0.65237-0
/content/journal/ijsem/10.1099/ijs.0.65237-0
/content/journal/ijsem/10.1099/ijs.0.65237-0
Loading

Data & Media loading...

Supplements

vol. , part 1, pp.257–261

Morphology of strain HN-182 grown at 30 °C on marine broth 2216 (Difco) for 1 day.

Polar lipids examined by two-dimensional TLC.

[single PDF](905 KB)

PDF

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error