1887

Abstract

A motile, rod-shaped bacterium, designated strain WPCB189, was isolated from fresh water collected from the Woopo wetland (Republic of Korea). The cells were found to be Gram-negative, aerobic and catalase- and oxidase-positive. A phylogenetic analysis based on 16S rRNA gene sequences showed that this isolate forms a lineage within the genus , showing sequence similarities of 89.7–96.4 % with respect to recognized species of the genus, and represents a novel member of this genus. The major fatty acids were iso-C 2-OH and/or C 7 (summed feature 3, 29.7 %), iso-C (26.4 %) and iso-C 3-OH (10.1 %). The DNA G+C content was 38.0 mol%. On the basis of data obtained from this polyphasic taxonomic study, strain WPCB189 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WPCB189 (=KCTC 12536=NBRC 101153).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65186-0
2007-09-01
2019-10-17
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/9/2079.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65186-0&mimeType=html&fmt=ahah

References

  1. Chun, J. & Goodfellow, M. ( 1995; ). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45, 240–245.[CrossRef]
    [Google Scholar]
  2. Chun, J., Bae, K. S., Moon, E. Y., Jung, S. O., Lee, H. K. & Kim, S. J. ( 2000; ). Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol 50, 1909–1913.
    [Google Scholar]
  3. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  4. Fitch, W. M. & Margoliash, E. ( 1967; ). Construction of phylogenetic trees. Science 155, 279–284.[CrossRef]
    [Google Scholar]
  5. Gallego, V., Garcia, M. T. & Ventosa, A. ( 2006; ). Pedobacter aquatilis sp. nov., isolated from drinking water, and emended description of the genus Pedobacter. Int J Syst Evol Microbiol 56, 1853–1858.[CrossRef]
    [Google Scholar]
  6. Hwang, C. Y., Choi, D. H. & Cho, B. C. ( 2006; ). Pedobacter roseus sp. nov., isolated from a hypertrophic pond, and emended description of the genus Pedobacter. Int J Syst Evol Microbiol 56, 1831–1836.[CrossRef]
    [Google Scholar]
  7. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  8. Kovács, N. ( 1956; ). Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178, 703
    [Google Scholar]
  9. Margesin, R., Spröer, C., Schumann, P. & Schinner, F. ( 2003; ). Pedobacter cryoconitis sp. nov., a facultative psychrophile from alpine glacier cryoconite. Int J Syst Evol Microbiol 53, 1291–1296.[CrossRef]
    [Google Scholar]
  10. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  11. NCCLS ( 2003; ). Performance standards for antimicrobial disk susceptibility tests, 8th edn. Approved Standard, M2-A8. Wayne, PA: National Committee for Clinical Laboratory Standards.
  12. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  13. Shivaji, S., Chaturvedi, P., Reddy, G. S. N. & Suresh, K. ( 2005; ). Pedobacter himalayensis sp. nov., from the Hamta glacier located in the Himalayan mountain ranges of India. Int J Syst Evol Microbiol 55, 1083–1088.[CrossRef]
    [Google Scholar]
  14. Staley, J. T. ( 1968; ). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95, 1921–1944.
    [Google Scholar]
  15. Steyn, P. L., Segers, P., Vancanneyt, M., Sandra, P., Kersters, K. & Joubert, J. J. ( 1998; ). Classification of heparinolytic bacteria into a new genus, Pedobacter, comprising four species: Pedobacter heparinus comb. nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov. Proposal of the family Sphingobacteriaceae fam. nov. Int J Syst Bacteriol 48, 165–177.[CrossRef]
    [Google Scholar]
  16. Swofford, D. L. ( 1998; ). paup – Phylogenetic analysis using parsimony, version 4. Sunderland, MA: Sinauer Associates.
  17. Takeuchi, M. & Yokota, A. ( 1992; ). Proposals of Sphingobacterium faecium sp. nov., Sphingobacterium piscium sp. nov., Sphingobacterium heparinum comb. nov., Sphingobacterium thalpophilum comb. nov., and two genospecies of the genus Sphingobacterium and synonymy of Flavobacterium yabuuchiae and Sphingobacterium spiritivorum. J Gen Appl Microbiol 38, 465–482.[CrossRef]
    [Google Scholar]
  18. Ten, L. N., Liu, Q.-M., Im, W.-T., Lee, M., Yang, D.-C. & Lee, S.-T. ( 2006; ). Pedobacter ginsengisoli sp. nov., a DNase-producing bacterium isolated from soil of a ginseng field in South Korea. Int J Syst Evol Microbiol 56, 2565–2570.[CrossRef]
    [Google Scholar]
  19. Vanparys, B., Heylen, K., Lebbe, L. & Vos, P. ( 2005; ). Pedobacter caeni sp. nov., a novel species isolated from a nitrifying inoculum. Int J Syst Evol Microbiol 55, 1315–1318.[CrossRef]
    [Google Scholar]
  20. Yoon, J. H., Lee, M. H., Kang, S. J., Park, S. Y. & Oh, T. K. ( 2006; ). Pedobacter sandarakinus sp. nov., isolated from soil. Int J Syst Evol Microbiol 56, 1273–1277.[CrossRef]
    [Google Scholar]
  21. Yoon, M.-H., Ten, L. N., Im, W.-T. & Lee, S.-T. ( 2007; ). Pedobacter panaciterrae sp. nov., isolated from soil in South Korea. Int J Syst Evol Microbiol 57, 381–386.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65186-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65186-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error