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Abstract

Strain c14, originally isolated from surface water of a freshwater pond located in Pingtung (southern Taiwan) used for culture of Pacific white shrimp (), was subjected to a polyphasic taxonomic approach. The strain exhibited strong chitinolytic activity and was able to grow under aerobic and anaerobic conditions by utilizing chitin exclusively as the carbon, nitrogen and energy source. Phylogenetic analysis of the 16S rRNA gene sequence revealed a clear affiliation of the proposed bacterium to the , most closely related to S1, WB 3.4-79 and KM-45, with 94.6, 93.6 and 92.9 % 16S rRNA gene sequence similarity, respectively. The predominant fatty acids detected in cells of strain c14 were C, C 7 and summed feature 3 (C 7 and/or C iso 2-OH). The G+C content of the genomic DNA was 69.5 (±1.0) mol%. Biochemical, physiological, chemotaxonomic and phylogenetic analyses showed that strain c14 could not be assigned to any known genus of the . Therefore, strain c14 is classified within a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is c14 (=LMG 23346 =BCRC 17533).

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2007-12-01
2019-10-14
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vol. , part 12, pp. 2854 - 2860

HPLC profile of chitin degraded by strain c14 after cultivation in CB medium. [PDF](21 KB)



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