1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 57, Issue 9

sp. nov., a moderate halophile from a soda lake in Inner Mongolia, China Free

Abstract

A Gram-positive, moderately halophilic, spore-forming bacterium, designated strain CG-15, was isolated from a soda lake, Lake Chagannor, in the Inner Mongolia Autonomous Region, China. The cells were found to be motile short rods with ellipsoidal, terminal and deforming endospores. Strain CG-15, a facultatively anaerobic bacterium, grew at pH 5.8–11.0 (optimally at pH 8.5), at 6–40 °C (optimally at 37 °C) and at salinities of 3–20 % (w/v) total salts (optimally at 7 % w/v). On the basis of the results of 16S rRNA gene sequence analysis, strain CG-15 was shown to belong to the genus (phylum ), showing the greatest phylogenetic similarity with respect to (96.0 %). The DNA G+C content of the novel isolate was found to be 53.8 mol%. The major cellular fatty acids of strain CG-15 were anteiso-C, iso-C and anteiso-C, and its polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three different unidentified phospholipids. The analysis of the quinones showed that MK-7 was the major menaquinone. The peptidoglycan type was A1, with -diaminopimelic acid as the diagnostic diamino acid. On the basis of the data from this polyphasic study, strain CG-15 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CG-15 (=CCM 7371=CECT 7153=CGMCC 1.6292=DSM 18086).

  • Published Online:
SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65162-0
2007-09-01
2022-01-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/9/2084.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65162-0&mimeType=html&fmt=ahah

References

  1. Albert R. A., Archambault J., Rosselló-Mora R., Tindall B. J., Matheny M. 2005; Bacillus acidicola sp. nov., a novel mesophilic, acidophilic species isolated from acidic Sphagnum peat bogs in Wisconsin. Int J Syst Evol Microbiol 55:2125–2130 [CrossRef]
     [Google Scholar]
  2. Arahal D. R., Ventosa A. 2002 Moderately halophilic and halotolerant species of Bacillus and related genera.In Applications and Systematics of Bacillus and Relatives, pp. 83–99. Edited by Berkeley R., Heyndrickx M., Logan N., De Vos P. Oxford: Blackwell;
  3. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D. 1991; Phylogenetic heterogeneity of the genus Bacillus as revealed by comparative analysis of small-subunit ribosomal-RNA sequences. Lett Appl Microbiol 13:202–206
     [Google Scholar]
  4. Duckworth A. W., Grant W. D., Jones B. E., van Steenbergen R. 1996; Phylogenetic diversity of soda lake alkaliphiles. FEMS Microbiol Ecol 19:181–191 [CrossRef]
     [Google Scholar]
  5. Dussault H. P. 1955; An improved technique for staining red halophilic bacteria. J Bacteriol 70:484–485
     [Google Scholar]
  6. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
     [Google Scholar]
  7. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
     [Google Scholar]
  8. García M. T., Ventosa A., Ruiz-Berraquero F., Kocur M. 1987; Taxonomic study and amended description of Vibrio costicola . Int J Syst Bacteriol 37:251–256 [CrossRef]
     [Google Scholar]
  9. Gomori G. 1955; Preparation of buffers for use in enzyme studies. Methods Enzymol 1:138–146
     [Google Scholar]
  10. Heyrman J., Vanparys B., Logan N. A., Balcaen A., Rodriguez-Diaz M., Felske A., De Vos P. 2004; Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus botaviensis sp. nov.and Bacillus drentensis sp. nov., from the Drentse A grasslands. Int J Syst Evol Microbiol 54:47–57 [CrossRef]
     [Google Scholar]
  11. Heyrman J., Rodriguez-Diaz M., Devos J., Felske A., Logan N. A., De Vos P. 2005; Bacillus novalis sp. nov., Bacillus arvi sp. nov. and Bacillus humi sp. nov., isolated from soil. Int J Syst Evol Microbiol 55:111–117 [CrossRef]
     [Google Scholar]
  12. Lim J.-M., Jeon C. O., Kim C.-J. 2006a; Bacillus taeanensis sp. nov., a halophilic Gram-positive bacterium from a solar saltern in Korea. Int J Syst Evol Microbiol 56:2903–2908 [CrossRef]
     [Google Scholar]
  13. Lim J.-M., Jeon C. O., Lee J. C., Ju Y. J., Park D. J., Kim C.-J. 2006b; Bacillus koreensis sp. nov., a spore-forming bacterium, isolated from the rhizosphere of willow roots in Korea. Int J Syst Evol Microbiol 56:59–63 [CrossRef]
     [Google Scholar]
  14. Lim J.-M., Jeon C. O., Lee S.-M., Xu L. H., Jiang C.-L., Kim C.-J. 2006c; Bacillus salarius sp. nov., a halophilic, spore-forming bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 56:373–377 [CrossRef]
     [Google Scholar]
  15. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar Buchner A., Lai T., Steppi S., Jobb G. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
     [Google Scholar]
  16. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
     [Google Scholar]
  17. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
     [Google Scholar]
  18. Nielsen P., Fritze D., Priest F. G. 1995; Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141:1745–1761 [CrossRef]
     [Google Scholar]
  19. Owen R. J., Hill L. R. 1979; The estimation of base compositions, base pairing and genome size of bacterial deoxyribonucleic acids. In Identification Methods for Microbiologists , 2nd edn. pp 217–296 Edited by Skinner F. A., Lovelock D. W. London: Academic Press;
     [Google Scholar]
  20. Priest F. G., Goodfellow M., Todd C. 1988; A numerical classification of the genus Bacillus . J Gen Microbiol 134:1847–1882
     [Google Scholar]
  21. Quesada E., Ventosa A., Ruiz-Berraquero F., Ramos-Cormenzana A. 1984; Deleya halophila , a new species of moderately halophilic bacteria. Int J Syst Bacteriol 34:287–292 [CrossRef]
     [Google Scholar]
  22. Romano I., Lama L., Nicolaus B., Gambacorta A., Giordano A. 2005; Bacillus saliphilus sp. nov., isolated from a mineral pool in Campania, Italy. Int J Syst Evol Microbiol 55:159–163 [CrossRef]
     [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
     [Google Scholar]
  24. Schlesner H., Lawson P. A., Collins M. D., Weiss N., Wehmeyer U., Völker H., Thomm M. 2001; Filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with Orn-d-Glu-type peptidoglycan. Int J Syst Evol Microbiol 51:425–431
     [Google Scholar]
  25. Spring S., Ludwig W., Marquez M. C., Ventosa A., Schleifer K.-H. 1996; Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp.nov. and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov. Int J Syst Bacteriol 46:492–496 [CrossRef]
     [Google Scholar]
  26. Stackebrandt E., Ebers J. 2006; Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155
     [Google Scholar]
  27. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
     [Google Scholar]
  28. Stackebrandt E., Fredericksen W., Garrity G. M., Grimont P. A. D., Kämpfer P., Maiden M. C. J., Nesme X., Rosselló-Mora R., Swings J. other authors 2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047 [CrossRef]
     [Google Scholar]
  29. Ventosa A., Quesada E., Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. 1982; Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128:1959–1968
     [Google Scholar]
  30. Ventosa A., Nieto J. J., Oren A. 1998; Biology of moderately halophilic aerobic bacteria. Microbiol Mol Biol Rev 62:504–544
     [Google Scholar]
  31. Wieser M., Worliczek H., Kämpfer P., Busse H.-J. 2005; Bacillus herbersteinensis sp. nov. Int J Syst Evol Microbiol 55:2119–2123 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65162-0
Loading
Bacillus chagannorensis sp. nov., a moderate halophile from a soda lake in Inner Mongolia, China
Int J Syst Evol Microbiol 57, 2084 (2007); https://doi.org/10.1099/ijs.0.65162-0
/content/journal/ijsem/10.1099/ijs.0.65162-0
/content/journal/ijsem/10.1099/ijs.0.65162-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error