1887

International Journal of Systematic and Evolutionary Microbiology

Volume 57, Issue 9

sp. nov., a moderate halophile from a soda lake in Inner Mongolia, China Free

Abstract

A Gram-positive, moderately halophilic, spore-forming bacterium, designated strain CG-15, was isolated from a soda lake, Lake Chagannor, in the Inner Mongolia Autonomous Region, China. The cells were found to be motile short rods with ellipsoidal, terminal and deforming endospores. Strain CG-15, a facultatively anaerobic bacterium, grew at pH 5.8–11.0 (optimally at pH 8.5), at 6–40 °C (optimally at 37 °C) and at salinities of 3–20 % (w/v) total salts (optimally at 7 % w/v). On the basis of the results of 16S rRNA gene sequence analysis, strain CG-15 was shown to belong to the genus (phylum ), showing the greatest phylogenetic similarity with respect to (96.0 %). The DNA G+C content of the novel isolate was found to be 53.8 mol%. The major cellular fatty acids of strain CG-15 were anteiso-C, iso-C and anteiso-C, and its polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three different unidentified phospholipids. The analysis of the quinones showed that MK-7 was the major menaquinone. The peptidoglycan type was A1, with -diaminopimelic acid as the diagnostic diamino acid. On the basis of the data from this polyphasic study, strain CG-15 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CG-15 (=CCM 7371=CECT 7153=CGMCC 1.6292=DSM 18086).

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65162-0
2007-09-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/9/2084.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65162-0&mimeType=html&fmt=ahah

References

  1. Albert, R. A., Archambault, J., Rosselló-Mora, R., Tindall, B. J. & Matheny, M. ( 2005; ). Bacillus acidicola sp. nov., a novel mesophilic, acidophilic species isolated from acidic Sphagnum peat bogs in Wisconsin. Int J Syst Evol Microbiol 55, 2125–2130.[CrossRef]
    [Google Scholar]
  2. Arahal, D. R. & Ventosa, A. ( 2002; ). Moderately halophilic and halotolerant species of Bacillus and related genera. In Applications and Systematics of Bacillus and Relatives, pp. 83–99. Edited by R. Berkeley, M. Heyndrickx, N. Logan & P. De Vos. Oxford: Blackwell.
  3. Ash, C., Farrow, J. A. E., Wallbanks, S. & Collins, M. D. ( 1991; ). Phylogenetic heterogeneity of the genus Bacillus as revealed by comparative analysis of small-subunit ribosomal-RNA sequences. Lett Appl Microbiol 13, 202–206.
     [Google Scholar]
  4. Duckworth, A. W., Grant, W. D., Jones, B. E. & van Steenbergen, R. ( 1996; ). Phylogenetic diversity of soda lake alkaliphiles. FEMS Microbiol Ecol 19, 181–191.[CrossRef]
    [Google Scholar]
  5. Dussault, H. P. ( 1955; ). An improved technique for staining red halophilic bacteria. J Bacteriol 70, 484–485.
     [Google Scholar]
  6. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  7. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  8. García, M. T., Ventosa, A., Ruiz-Berraquero, F. & Kocur, M. ( 1987; ). Taxonomic study and amended description of Vibrio costicola. Int J Syst Bacteriol 37, 251–256.[CrossRef]
    [Google Scholar]
  9. Gomori, G. ( 1955; ). Preparation of buffers for use in enzyme studies. Methods Enzymol 1, 138–146.
     [Google Scholar]
  10. Heyrman, J., Vanparys, B., Logan, N. A., Balcaen, A., Rodriguez-Diaz, M., Felske, A. & De Vos, P. ( 2004; ). Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus botaviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A grasslands. Int J Syst Evol Microbiol 54, 47–57.[CrossRef]
    [Google Scholar]
  11. Heyrman, J., Rodriguez-Diaz, M., Devos, J., Felske, A., Logan, N. A. & De Vos, P. ( 2005; ). Bacillus novalis sp. nov., Bacillus arvi sp. nov. and Bacillus humi sp. nov., isolated from soil. Int J Syst Evol Microbiol 55, 111–117.[CrossRef]
    [Google Scholar]
  12. Lim, J.-M., Jeon, C. O. & Kim, C.-J. ( 2006a; ). Bacillus taeanensis sp. nov., a halophilic Gram-positive bacterium from a solar saltern in Korea. Int J Syst Evol Microbiol 56, 2903–2908.[CrossRef]
    [Google Scholar]
  13. Lim, J.-M., Jeon, C. O., Lee, J. C., Ju, Y. J., Park, D. J. & Kim, C.-J. ( 2006b; ). Bacillus koreensis sp. nov., a spore-forming bacterium, isolated from the rhizosphere of willow roots in Korea. Int J Syst Evol Microbiol 56, 59–63.[CrossRef]
    [Google Scholar]
  14. Lim, J.-M., Jeon, C. O., Lee, S.-M., Xu, L. H., Jiang, C.-L. & Kim, C.-J. ( 2006c; ). Bacillus salarius sp. nov., a halophilic, spore-forming bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 56, 373–377.[CrossRef]
    [Google Scholar]
  15. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T. & Steppi, SJobb, G. & other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  16. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  17. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  18. Nielsen, P., Fritze, D. & Priest, F. G. ( 1995; ). Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141, 1745–1761.[CrossRef]
    [Google Scholar]
  19. Owen, R. J. & Hill, L. R. ( 1979; ). The estimation of base compositions, base pairing and genome size of bacterial deoxyribonucleic acids. In Identification Methods for Microbiologists, 2nd edn, pp. 217–296. Edited by F. A. Skinner & D. W. Lovelock. London: Academic Press.
  20. Priest, F. G., Goodfellow, M. & Todd, C. ( 1988; ). A numerical classification of the genus Bacillus. J Gen Microbiol 134, 1847–1882.
     [Google Scholar]
  21. Quesada, E., Ventosa, A., Ruiz-Berraquero, F. & Ramos-Cormenzana, A. ( 1984; ). Deleya halophila, a new species of moderately halophilic bacteria. Int J Syst Bacteriol 34, 287–292.[CrossRef]
    [Google Scholar]
  22. Romano, I., Lama, L., Nicolaus, B., Gambacorta, A. & Giordano, A. ( 2005; ). Bacillus saliphilus sp. nov., isolated from a mineral pool in Campania, Italy. Int J Syst Evol Microbiol 55, 159–163.[CrossRef]
    [Google Scholar]
  23. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
     [Google Scholar]
  24. Schlesner, H., Lawson, P. A., Collins, M. D., Weiss, N., Wehmeyer, U., Völker, H. & Thomm, M. ( 2001; ). Filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with Orn-d-Glu-type peptidoglycan. Int J Syst Evol Microbiol 51, 425–431.
     [Google Scholar]
  25. Spring, S., Ludwig, W., Marquez, M. C., Ventosa, A. & Schleifer, K.-H. ( 1996; ). Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp. nov. and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov. Int J Syst Bacteriol 46, 492–496.[CrossRef]
    [Google Scholar]
  26. Stackebrandt, E. & Ebers, J. ( 2006; ). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33, 152–155.
     [Google Scholar]
  27. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  28. Stackebrandt, E., Fredericksen, W., Garrity, G. M., Grimont, P. A. D., Kämpfer, P., Maiden, M. C. J., Nesme, X., Rosselló-Mora, R., Swings, J. & other authors ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52, 1043–1047.[CrossRef]
    [Google Scholar]
  29. Ventosa, A., Quesada, E., Rodriguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Cormenzana, A. ( 1982; ). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128, 1959–1968.
     [Google Scholar]
  30. Ventosa, A., Nieto, J. J. & Oren, A. ( 1998; ). Biology of moderately halophilic aerobic bacteria. Microbiol Mol Biol Rev 62, 504–544.
     [Google Scholar]
  31. Wieser, M., Worliczek, H., Kämpfer, P. & Busse, H.-J. ( 2005; ). Bacillus herbersteinensis sp. nov. Int J Syst Evol Microbiol 55, 2119–2123.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65162-0
Loading
Bacillus chagannorensis sp. nov., a moderate halophile from a soda lake in Inner Mongolia, China
Int J Syst Evol Microbiol 57, 2084 (2007); https://doi.org/10.1099/ijs.0.65162-0
/content/journal/ijsem/10.1099/ijs.0.65162-0
/content/journal/ijsem/10.1099/ijs.0.65162-0
Loading

Data & Media loading...

Supplements

vol. , part 9, pp. 2084 - 2088

Phase-contrast photomicrograph of cells of strain CG-15 , showing spore-forming rods. [PDF](75 KB)

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error