1887

Abstract

Two facultatively aerobic, heterotrophic bacteria capable of degrading pectin, xylan, laminarin and some other polysaccharides were obtained from the acidic peat bog Bakchar, in western Siberia, Russia, and were designated strains TPT18 and TPT56. Cells of these isolates are Gram-negative, non-motile, long rods that are covered by large capsules. On ageing, they transform into spherical L-forms. Strains TPT18 and TPT56 are acido- and psychrotolerant organisms capable of growth at pH 4.2–8.2 (with an optimum at pH 6.0–6.5) and at 2–33 °C (with an optimum at 20 °C). The major fatty acids are iso-C, anteiso-C, iso-C 3-OH and summed feature 3 (iso-C 2-OH and/or C 7); the quinones are MK-7 and MK-6. Comparative 16S rRNA gene sequence analysis revealed that the novel strains share 97 % sequence similarity and belong to the family ; however, they are related only distantly to members of the genera (91.8–93.3 % similarity) and (89.6–91.2 % similarity). The DNA G+C content of strains TPT18 and TPT56 is 42.4 and 46.1 mol%, respectively. The low DNA–DNA hybridization value (42 %) and a number of phenotypic differences between strains TPT18 and TPT56 indicated that they represent two separate species. Since the two isolates are clearly distinct from all currently described members of the family , we propose a novel genus, gen. nov., containing two novel species, sp. nov. and sp. nov. The type strains of and are respectively TPT18 (=ATCC BAA-1391 =VKM B-2447) and TPT56 (=ATCC BAA-1394 =VKM B-2446).

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2007-10-01
2020-01-17
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vol. , part 10, pp. 2349 - 2354

Phenotypic characteristics of strains TPT18 and TPT56. [PDF](20 KB)



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