1887

Abstract

Selective culture of human carious dentine for strains resulted in the isolation of two strains of a Gram-negative, coccus-shaped bacterium that has not been described previously. Comparative 16S rRNA and gene sequence analysis indicated that the two strains were homogeneous and comprised a distinct lineage within the genus , phylogenetically most closely related to . This was supported by DNA–DNA hybridization, which showed clearly that the two strains were similar and distinct from other species, and the production of major cellular fatty acids (C and C 8), which is consistent with other members of the genus . Based on these observations, strains RBV81 and RBV106 represent a novel species, for which the name sp. nov. is proposed, with the type strain RBV106 (=CIP 109448 =CCUG 54362 =DSM 19009).

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2007-12-01
2019-09-16
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References

  1. Arif, N., Do, T., Byun, R., Sheehy, E., Clarke, D., Gilbert, S. C. & Beighton, D. ( 2008; ). Veillonella rogosae sp. nov., an anaerobic, Gram-negative coccus isolated from the dental plaque of caries-free children. Int J Syst Evol Microbiol 58 (in press).
    [Google Scholar]
  2. Bladen, H. A. & Mergenhagen, S. E. ( 1964; ). Ultrastructure of Veillonella and morphological correlation of an outer membrane with particles associated with endotoxic activity. J Bacteriol 88, 1482–1492.
    [Google Scholar]
  3. Carlier, J.-P. ( 1985; ). Gas chromatography of fermentation products: its application in diagnosis of anaerobic bacteria. Bull Inst Pasteur 83, 57–69.
    [Google Scholar]
  4. Chhour, K. L., Nadkarni, M. A., Byun, R., Martin, F. E., Jacques, N. A. & Hunter, N. ( 2005; ). Molecular analysis of microbial diversity in advanced caries. J Clin Microbiol 43, 843–849.[CrossRef]
    [Google Scholar]
  5. Christensen, H., Angen, Ø., Mutters, R., Olsen, J. E. & Bisgaard, M. ( 2000; ). DNA–DNA hybridization determined in micro-wells using covalent attachment of DNA. Int J Syst Evol Microbiol 50, 1095–1102.[CrossRef]
    [Google Scholar]
  6. Edwardsson, S. ( 1974; ). Bacteriological studies on deep areas of carious dentine. Odontol Revy Suppl 32, 1–143.
    [Google Scholar]
  7. Foubert, E. L. & Douglas, H. C. ( 1948; ). Studies on the anaerobic micrococci. II. The fermentation of lactate by Micrococcus lactilyticus. J Bacteriol 56, 35–36.
    [Google Scholar]
  8. Holdeman, L. V., Cato, E. P. & Moore, W. E. C. ( 1977; ). Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University.
  9. Hoshino, E. ( 1985; ). Predominant obligate anaerobes in human carious dentin. J Dent Res 64, 1195–1198.[CrossRef]
    [Google Scholar]
  10. Hoshino, E., Karino, H. & Yamada, T. ( 1981; ). Lactate metabolism by human dental plaque and Veillonella under aerobic and anaerobic conditions. Arch Oral Biol 26, 17–22.[CrossRef]
    [Google Scholar]
  11. Jumas-Bilak, E., Carlier, J.-P., Jean-Pierre, H., Teyssier, C., Gay, B., Campos, J. & Marchandin, H. ( 2004; ). Veillonella montpellierensis sp. nov., a novel, anaerobic, Gram-negative coccus isolated from human clinical samples. Int J Syst Evol Microbiol 54, 1311–1316.[CrossRef]
    [Google Scholar]
  12. Marchandin, H., Teyssier, C., Siméon de Buochberg, M., Jean-Pierre, H., Carriere, C. & Jumas-Bilak, E. ( 2003; ). Intra-chromosomal heterogeneity between the four 16S rRNA gene copies in the genus Veillonella: implications for phylogeny and taxonomy. Microbiology 149, 1493–1501.[CrossRef]
    [Google Scholar]
  13. Martin, F. E., Nadkarni, M. A., Jacques, N. A. & Hunter, N. ( 2002; ). Quantitative microbiological study of human carious dentine by culture and real-time PCR: association of anaerobes with histopathological changes in chronic pulpitis. J Clin Microbiol 40, 1698–1704.[CrossRef]
    [Google Scholar]
  14. Mays, T. D., Holdeman, L. V., Moore, W. E. C., Rogosa, M. & Johnson, J. L. ( 1982; ). Taxonomy of the genus Veillonella Prévot. Int J Syst Bacteriol 32, 28–36.[CrossRef]
    [Google Scholar]
  15. Mikx, F. H., van der Hoeven, J. S., Konig, K. G., Plasschaert, A. J. & Guggenheim, B. ( 1972; ). Establishment of defined microbial ecosystems in germ-free rats. I. The effect of the interactions of Streptococcus mutans or Streptococcus sanguis with Veillonella alcalescens on plaque formation and caries activity. Caries Res 6, 211–223.[CrossRef]
    [Google Scholar]
  16. Ng, S. K. & Hamilton, I. R. ( 1971; ). Lactate metabolism by Veillonella parvula. J Bacteriol 105, 999–1005.
    [Google Scholar]
  17. Paster, B. J., Boches, S. K., Galvin, J. L., Ericson, R. E., Lau, C. N., Levanos, V. A., Sahasrabudhe, A. & Dewhirst, F. E. ( 2001; ). Bacterial diversity in human subgingival plaque. J Bacteriol 183, 3770–3783.[CrossRef]
    [Google Scholar]
  18. Rogosa, M. ( 1956; ). A selective medium for the isolation and enumeration of the Veillonella from the oral cavity. J Bacteriol 72, 533–536.
    [Google Scholar]
  19. Rogosa, M. ( 1964; ). The genus Veillonella. I. General cultural, ecological, and biochemical considerations. J Bacteriol 87, 162–170.
    [Google Scholar]
  20. Rogosa, M. ( 1965; ). The genus Veillonella. IV. Serological groupings, and genus and species emendations. J Bacteriol 90, 704–709.
    [Google Scholar]
  21. Rogosa, M. ( 1984; ). Anaerobic Gram-negative cocci. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 680–685. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  22. Rogosa, M., Fitzgerald, R. J., MacKintosh, M. E. & Beaman, A. J. ( 1958; ). Improved medium for selective isolation of Veillonella. J Bacteriol 76, 455–456.
    [Google Scholar]
  23. Sato, T., Sato, M., Matsuyama, J. & Hoshino, E. ( 1997; ). PCR-restriction fragment length polymorphism analysis of genes coding for 16S rRNA in Veillonella spp. Int J Syst Bacteriol 47, 1268–1270.[CrossRef]
    [Google Scholar]
  24. van der Hoeven, J. S., Toorop, A. I. & Mikx, R. H. ( 1978; ). Symbiotic relationship of Veillonella alcalescens and Streptococcus mutans in dental plaque in gnotobiotic rats. Caries Res 12, 142–147.[CrossRef]
    [Google Scholar]
  25. Veys, A., Callewaert, W., Waelkens, E. & Van den Abbeele, K. ( 1989; ). Application of gas-liquid chromatography to the routine identification of nonfermenting Gram-negative bacteria in clinical specimens. J Clin Microbiol 27, 1538–1542.
    [Google Scholar]
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vol. , part 12, pp. 2844 - 2848

DNA–DNA hybridization and 16S rRNA gene sequence identity between strains RBV106 and RBV81 ( sp. nov.) and strains of other species. [PDF](30 KB)



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