1887

Abstract

A Gram-positive, aerobic, non-motile, mesophilic strain, djl-6, able to degrade carbendazim, was isolated from a carbendazim-contaminated soil sample from Jiangsu province, China. The taxonomic position of this isolate was analysed by using a polyphasic approach. Chemotaxonomic analysis including peptidoglycan type, diagnostic sugar composition, fatty acid profile, menaquinones, polar lipids and mycolic acids showed that the characteristics of strain djl-6 were in good agreement with those of the genus . DNA–DNA hybridization showed that it had low genomic relatedness with DSM 44587 (31.8 %), DSM 43066 (23.8 %) and DSM 43954 (17.7 %), the three type strains to which strain djl-6 was most closely related based on 16S rRNA gene sequence analysis (99.78, 99.25 and 98.91 % similarity, respectively). Based on the phenotypic properties and DNA–DNA hybridization data, strain djl-6 (=CGMCC 1.6580 =KCTC 19205) is proposed as the type strain of a novel species, sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65095-0
2007-12-01
2020-01-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/12/2754.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65095-0&mimeType=html&fmt=ahah

References

  1. Buck, J. D. ( 1982; ). Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44, 992–993.
    [Google Scholar]
  2. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  3. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  4. Ebersole, L. L. ( 1992; ). Acid-fast stain procedures. In Clinical Microbiology Procedures Handbook, pp. 3.5.1–3.5.11. Edited by H. D. Isenberg. Washington, DC: American Society for Microbiology.
  5. Euzéby, J. P. ( 2007; ). Rhodococcus Zopf 1891, genus. In List of Prokaryotic Names with Standing in Nomenclature. http://www.bacterio.cict.fr/qr/rhodococcus.html. Accessed 27 September 2007.
  6. Goodfellow, M. & Alderson, G. ( 1977; ). The actinomycete-genus Rhodococcus: a home for the ‘rhodochrous’ complex. J Gen Microbiol 100, 99–122.[CrossRef]
    [Google Scholar]
  7. Gordon, R. E. ( 1966; ). Some criteria for the recognition of Nocardia madurae (Vincent) Blanchard. J Gen Microbiol 45, 355–364.[CrossRef]
    [Google Scholar]
  8. Gordon, R. E. ( 1967; ). The taxonomy of soil bacteria. In The Ecology of Soil Bacteria, pp. 293–321. Edited by T. R. G. Gray & D. Parkinson. Liverpool: Liverpool University Press.
  9. Hasegawa, T., Takizaea, M. & Tanida, S. ( 1983; ). A rapid analysis for chemical grouping aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef]
    [Google Scholar]
  10. Holtman, M. A. & Kobayashi, D. Y. ( 1997; ). Identification of Rhodococcus erythropolis isolates capable of degrading the fungicide carbendazim. Appl Microbiol Biotechnol 47, 578–582.[CrossRef]
    [Google Scholar]
  11. Kroppenstedt, R. M. ( 1982; ). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5, 2359–2367.[CrossRef]
    [Google Scholar]
  12. Lanyi, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  13. Lechevalier, M. P. & Lechevalier, H. ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef]
    [Google Scholar]
  14. Li, Y., Kawamura, Y., Fujiwara, N., Naka, T., Liu, H. S., Huang, X. X., Kobayashi, K. & Ezaki, T. ( 2004; ). Rothia aeria sp. nov., Rhodococcus baikonurensis sp. nov. and Arthrobacter russicus sp. nov., isolated from air in the Russian space laboratory Mir. Int J Syst Evol Microbiol 54, 827–835.[CrossRef]
    [Google Scholar]
  15. Loeffelholz, M. J. & Scholl, D. R. ( 1989; ). Method for improved extraction of DNA from Nocardia asteroides. J Clin Microbiol 27, 1880–1881.
    [Google Scholar]
  16. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  17. Mazellier, P., Leroy, É., De Laat, J. & Legube, B. ( 2003; ). Degradation of carbendazim by UV/H2O2 investigated by kinetic modelling. Environ Chem Lett 1, 68–72.[CrossRef]
    [Google Scholar]
  18. Minnikin, D. E., Alshamaony, L. & Goodfellow, M. ( 1975; ). Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88, 200–204.[CrossRef]
    [Google Scholar]
  19. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  20. Shao, Z. Q., Seffens, W., Mulbry, W. & Behki, R. M. ( 1995; ). Cloning and expression of the s-triazine hydrolase gene (trzA) from Rhodococcus corallinus and development of Rhodococcus recombinant strains capable of dealkylating and dechlorinating the herbicide atrazine. J Bacteriol 177, 5748–5755.
    [Google Scholar]
  21. Shirling, E. D. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  22. Tsukamura, M. ( 1974; ). A further numerical taxonomic study of the rhodochrous group. Jpn J Microbiol 18, 37–44.[CrossRef]
    [Google Scholar]
  23. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  24. WHO ( 1993; ). Environmental Health Criteria 149. Carbendazim. http://www.inchem.org/documents/ehc/ehc/ehc149.htm. Accessed 27 September 2007. Geneva: World Health Organization.
  25. Xu, J.-L., Gu, X.-Y., Shen, B., Wang, Z.-C., Wang, K. & Li, S.-P. ( 2006; ). Isolation and characterization of a carbendazim-degrading Rhodococcus sp. djl-6. Curr Microbiol 53, 72–76.[CrossRef]
    [Google Scholar]
  26. Zhang, J., Zhang, Y., Xiao, C., Liu, Z. & Goodfellow, M. ( 2002; ). Rhodococcus maanshanensis sp. nov., a novel actinomycete from soil. Int J Syst Evol Microbiol 52, 2121–2126.[CrossRef]
    [Google Scholar]
  27. Zhang, G.-S., Jia, X.-M., Cheng, T.-F., Ma, X.-H. & Zhao, Y.-H. ( 2005a; ). Isolation and characterization of a new carbendazim-degrading Ralstonia sp. strain. World J Microbiol Biotechnol 21, 265–269.[CrossRef]
    [Google Scholar]
  28. Zhang, Y.-Q., Li, W.-J., Kroppenstedt, R. M., Kim, C.-J., Chen, G.-Z., Park, D.-J., Xu, L.-H. & Jiang, C.-L. ( 2005b; ). Rhodococcus yunnanensis sp. nov., a mesophilic actinobacterium isolated from forest soil. Int J Syst Evol Microbiol 55, 1133–1137.[CrossRef]
    [Google Scholar]
  29. Zopf, W. ( 1891; ). Über Ausscheidung von Fettfarbstoffen (Lipochromen) seitens gewisser Spaltpilze. Ber Dtsch Bot Ges 9, 22–28 (in German).
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65095-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65095-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error