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Abstract

Three actinomycetes (strains HKI 0478, HKI 0479 and HKI 0480) isolated from the surfaces of rocks in the Feengrotten medieval alum slate mine (Thuringia, Germany) were examined in a polyphasic taxonomic study. The following morphological and chemotaxonomic features supported their classification as members of the genus : the presence of -diaminopimelic acid in the cell-wall peptidoglycan; glucose together with minor amounts of mannose and ribose as the whole-cell sugars; polar lipids comprising phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and unknown phospho- and glycolipids; fatty acid profiles characterized by the predominance of anteiso-C, iso-C and C 9-methyl; and the presence of MK-9(H) as the main menaquinone. The isolates had almost identical 16S rRNA gene sequences (99.9–100 %) and were most closely related to the type strains of (98.9 % sequence similarity), and (both 98.8 %). A wide range of genotypic and phenotypic markers as well as the low levels of DNA–DNA relatedness between strain HKI 0478 and the type strains of (41.3 %), (18.6 %) and (14.2 %) distinguished the novel strains from their closest phylogenetic neighbours. On the basis of these results, strain HKI 0478 represents a novel member of the genus , for which the name sp. nov. is proposed. The type strain is HKI 0478 (=DSM 18824 =JCM 14599).

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2007-09-01
2019-10-21
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vol. , part 9, pp. 1943 - 1947

Cellular fatty acid compositions (%) of strains HKI 0478 , HKI 0479 and HKI 0480 and related type strains. [PDF](16 KB)



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