A freshwater bacterium, designated IMCC1704, was isolated from a eutrophic pond. The strain was Gram-negative, oxidase-positive, catalase-negative, chemoheterotrophic and facultatively aerobic with cells that were motile rods with a single polar flagellum. Based on 16S rRNA gene sequence similarity analyses, the novel strain was most closely related to the genera (91.7 %), (89.0–91.2 %), (87.9–90.1 %), (87.2–89.5 %) and (87.8 %). Phylogenetic trees generated using 16S rRNA gene sequences showed that the novel isolate belonged to the family of the class and formed a distinct phyletic lineage within the family. The DNA G+C content of the strain was 63.1 mol% and the predominant constituents of the cellular fatty acids were C 7 and/or iso-C 2-OH (21.2 %), C 7 (12.8 %) and C 3-OH (12.3 %). These chemotaxonomic properties, together with several phenotypic characteristics, differentiated the novel strain from other members of the family . From the taxonomic data, which revealed the distant relationship of the new strain to the related genera, the strain should be classified as a novel genus and species in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of sp. nov. is IMCC1704 (=KCCM 42363=NBRC 102354).


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vol. , part 1, pp. 97 - 102

A transmission electron micrograph of a negatively stained cell of strain IMCC1704 . [ PDF] (111 KB)

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