1887

Abstract

A Gram-negative, motile, aerobic and oval- or rod-shaped bacterial strain, DS-56, which is phylogenetically closely related to the genus , was isolated from soil from Dokdo, Korea. Strain DS-56 grew optimally at pH 6.5–7.5 and 25 °C in the presence of 0.5 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain DS-56 joins the cluster comprising species, at a bootstrap resampling value of 100 %. The levels of 16S rRNA gene sequence similarity between strain DS-56 and the type strains of recognized species ranged from 95.4 to 96.4 %. Strain DS-56 contained Q-11 as the predominant ubiquinone, unlike species, which contain Q-10 as the predominant ubiquinone. The novel strain also contained some fatty acids, including branched and hydroxy fatty acids, that are not detected in species. The DNA G+C content of strain DS-56 was 66.2 mol%. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain DS-56 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DS-56 (=KCTC 12821=DSM 17955).

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2007-06-01
2020-01-27
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References

  1. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  2. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19, 161–203.
    [Google Scholar]
  3. Lanyi, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  4. Lee, K.-B., Liu, C.-T., Anzai, Y., Kim, H., Aono, T. & Oyaizu, H. ( 2005; ). The hierarchical system of the ‘Alphaproteobacteria’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov. Int J Syst Evol Microbiol 55, 1907–1919.[CrossRef]
    [Google Scholar]
  5. Nakagawa, Y., Sakane, T. & Yokota, A. ( 1996; ). Transfer of “Pseudomonas riboflavina” (Foster 1944), a gram-negative, motile rod with long-chain 3-hydroxy fatty acids, to Devosia riboflavina gen. nov., sp. nov., nom. rev. Int J Syst Bacteriol 46, 16–22.[CrossRef]
    [Google Scholar]
  6. Rivas, R., Willems, A., Subba-Rao, N. S., Mateos, P. F., Dazzo, F. B., Kroppenstedt, R. M., Martínez-Molina, E., Gillis, M. & Velázquez, E. ( 2003; ). Description of Devosia neptuniae sp. nov. that nodulates and fixes nitrogen in symbiosis with Neptunia natans, an aquatic legume from India. Syst Appl Microbiol 26, 47–53.[CrossRef]
    [Google Scholar]
  7. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  8. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  9. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  10. Vanparys, B., Heylen, K., Lebbe, L. & De Vos, P. ( 2005; ). Devosia limi sp. nov., isolated from a nitrifying inoculum. Int J Syst Evol Microbiol 55, 1997–2000.[CrossRef]
    [Google Scholar]
  11. Yoo, S.-H., Weon, H. Y., Kim, B. Y., Hong, S.-B., Kwon, S.-W., Cho, Y.-H., Go, S.-J. & Stackebrandt, E. ( 2006; ). Devosia soli sp. nov., isolated from greenhouse soil in Korea. Int J Syst Evol Microbiol 56, 2689–2692.[CrossRef]
    [Google Scholar]
  12. Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H. ( 1996; ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46, 502–505.[CrossRef]
    [Google Scholar]
  13. Yoon, J.-H., Lee, S. T. & Park, Y.-H. ( 1998; ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef]
    [Google Scholar]
  14. Yoon, J.-H., Kang, K. H. & Park, Y.-H. ( 2003; ). Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53, 449–454.[CrossRef]
    [Google Scholar]
  15. Yoon, J.-H., Kang, S.-J., Lee, S.-Y., Lee, M.-H. & Oh, T.-K. ( 2005a; ). Virgibacillus dokdonensis sp. nov., isolated from a Korean island, Dokdo, located at the edge of the East Sea in Korea. Int J Syst Evol Microbiol 55, 1833–1837.[CrossRef]
    [Google Scholar]
  16. Yoon, J.-H., Kang, S.-J., Lee, C.-H. & Oh, T.-K. ( 2005b; ). Dokdonia donghaensis gen. nov., sp. nov., isolated from sea water. Int J Syst Evol Microbiol 55, 2323–2328.[CrossRef]
    [Google Scholar]
  17. Yoon, J.-H., Kang, S.-J. & Oh, T.-K. ( 2006a; ). Dokdonella koreensis gen. nov., sp. nov., isolated from soil. Int J Syst Evol Microbiol 56, 145–150.[CrossRef]
    [Google Scholar]
  18. Yoon, J.-H., Kang, S.-J. & Oh, T.-K. ( 2006b; ). Variovorax dokdonensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 56, 811–814.[CrossRef]
    [Google Scholar]
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