1887

Abstract

A novel, Gram-negative bacterial strain, SUA2, isolated from groundwater, was characterized using a polyphasic approach. Cells are Gram-negative, non-spore-forming, straight to curved rods with a single polar flagellum. Strain SUA2 is oxidase- and catalase-positive and is able to fix nitrogen. Poly--hydroxybutyrate storage granules are produced. Dominant fatty acids when grown in R2A and VM ethanol media for 72 h at 37 °C are C, C 7c, C cyclo, C 3-OH, C 7c, C and C. DNA G+C content is 67.9 mol%. Phenotypic and phylogenetic data indicate that strain SUA2 is related to, but clearly differentiated from . Strain SUA2 is thus proposed as a novel species of the genus with the name sp. nov. The description of the genus is emended to include the characteristics of this novel species. The type strain of is SUA2 (=NRRL B-41660=DSM 18626=LMG 23819).

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2007-07-01
2019-10-21
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A table showing the fatty acid composition of strains SUA2 , 6a3 and PS grown in R2A (this study) or VM ethanol medium (DSMZ Identification Services) is available hereas an Acrobat PDF file.

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Morphological feature of SUA2 observed by (a) differential contrast microscopy and (b) transmission electron microscopy. Bars, 5 µm (a), 1 µm (b). Cells were grown on R2A agar plate at 30 °C for 4 days.

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SDS-PAGE showing whole-cell soluble protein patterns from (A) strain SUA2 , (B) 6a3 and (C) PS. Soluble proteins were extracted from the overnight grown cells in R2A liquid medium. S, Pre-stained SDS-PAGE standards (Bio-Rad).

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